Effect of an Iron-Chelator on Ascorbate-Induced Cytotoxicity

Sakagami, Hiroshi; Satoh, Kazue; Fukuchi, Kunihiko; Gomi, Kunihide; Takeda, Minoru

doi:10.1016/s0891-5849(96)00621-1

Cited by 28 publications

(20 citation statements)

References 21 publications

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“…Such cytotoxic phenomenon of ascorbate was also described by others [9,23], however the apoptosis-inhibiting effect of ascorbate was documented as well [24,25]. One study showed that not the ascorbate itself but the accompanying amount of generated H 2 O 2 , dependent on the media used, was responsible for the cytotoxic effect [26].…”

Section: Discussionmentioning

confidence: 64%

“…For healing of wounds and bone fractures collagen is essential particularly. The special importance of vitamin C in collagen biosynthesis was confirmed in the investigations of several research groups [9,21,27]. …”

Section: Discussionmentioning

confidence: 87%

“…Inhibition of bone formation and injury of connective tissue caused by a vitamin C deficiency is accompanied with a decreased collagen biosynthesis ([21], 27, [9]). Vitamin C plays an important role in hydroxylation of lysine and proline in the collagen biosynthesis, for example.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism behind the influence of ascorbic acid on the proliferation and differentiation of various cell types still remains unclear. Besides the promoting effect of ascorbate, it was also shown that ascorbate was cytotoxic for cells, inducing for example apoptosis in HL-60 cells [9]. It was shown that the ECM, especially collagen I, increases the expression of differentiation markers in different cell types.…”

Section: Introductionmentioning

confidence: 99%

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An in vitro study of osteoblast vitality influenced by the vitamins C and E

et al. 2012

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Vitamin C and vitamin E are known as important cellular antioxidants and are involved in several other non-antioxidant processes. Generally vitamin C and vitamin E are not synthesized by humans and therefore have to be applied by nutrition. The absence or deficiency of the vitamins can lead to several dysfunctions and even diseases (e.g. scurvy). The main interest in this study is that vitamin C and E are known to influence bone formation, e.g. vitamin C plays the key role in the synthesis of collagen, the major component of the extracellular bone matrix.In the present study we evaluate the effect of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) on the proliferation and differentiation of primary bovine osteoblasts in vitro. Starting from standard growth medium we minimized the foetal calf serum to reduce their stimulatory effect on proliferation.An improved growth and an increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin was observed while increasing the ascorbic acid concentration up to 200 μg/ml. Furthermore the effects of α-tocopherol on cell growth and cell differentiation were examined, whereby neither improved growth nor increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin were detected.Further investigations are necessary to target at better supportive effect of vitamins on bone regeneration, and healing.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An in vitro study of osteoblast vitality influenced by the vitamins C and E

et al. 2012

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“…The association of epidemiological/experimental evidence with antioxidant protection has been extensively investigated, but relationships are not yet clearly established 10. In addition, intervention trials have been disappointing, and a pro‐oxidant effect of some antioxidants has been reported 11, 12. Further study into the DNA protective or damaging effect of antioxidants on living cells is required to establish which dietary antioxidants may be beneficial and the concentration at which a possible pro‐oxidant effect may outweigh an antioxidant effect.…”

Section: Introductionmentioning

confidence: 99%

Highly Selective Dehydrogenative Silylation of Alkenes Catalyzed by Rhenium Complexes

Jiang

Blacque

Fox

et al. 2009

Chemistry A European J

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Single‐cell gel electrophoresis: a tool for investigation of DNA protection or damage mediated by dietary antioxidants

Szeto

2007

J Sci Food Agric

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One method of assessing DNA damage is the comet assay, which was developed in 1988. The comet assay enables the detection of DNA strand breaks in individual cells. This test has also been used to study the in vitro and in vivo genotoxic or genoprotective effects of certain agents such as dietary antioxidants. This paper aims to consolidate the antioxidant and pro-oxidant effects of a series of dietary agents which have been evaluated by comet assay.

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Effect of an Iron-Chelator on Ascorbate-Induced Cytotoxicity

Cited by 28 publications

References 21 publications

An in vitro study of osteoblast vitality influenced by the vitamins C and E

An in vitro study of osteoblast vitality influenced by the vitamins C and E

Highly Selective Dehydrogenative Silylation of Alkenes Catalyzed by Rhenium Complexes

Single‐cell gel electrophoresis: a tool for investigation of DNA protection or damage mediated by dietary antioxidants

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