Effect of Anacardic Acid against Thiram Induced Tibial Dyschondroplasia in Chickens via Regulation of Wnt4 Expression

Abstract

Tibial dyschondroplasia (TD) is a tibia bone problem in broilers. Anacardic acid (AA) is a traditional Chinese medicine, which is commonly used to treat arthritis in human. The purpose of the present study is to investigate the effect of AA against TD. A total of 300 day-old poultry birds were equally divided and distributed into three different groups: Control, TD and AA groups. The results showed that the feed conversion ratio was significantly lower in the TD group than control chickens. The tibia bone parameters including weight, length and width were of low quality in TD chickens, while the width of the tibial growth plate was enlarged remarkably. Whereas, in the AA treatment group, the tibia bone parameters showed improvement and tend to return to normal. The antioxidant parameters level of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total and antioxidant capacity (T-AOC) was significantly decreased, while malondialdehyde (MDA) level was increased significantly in TD affected chickens. AA administration restored the antioxidant parameters significantly. The gene expression revealed a decrease in Wnt4 expression in TD chickens as compared to control chickens, while AA treatment up-regulated the Wnt4 expression. The present study demonstrates that the AA plays an important role to prevent the lameness and restore the size of tibial growth plate of chickens by regulating the expression of Wnt4.

Osthole: A Coumarin Derivative Assuage Thiram-Induced Tibial Dyschondroplasia by Regulating BMP-2 and RUNX-2 Expressions in Chickens

“…The homogenate was then centrifuged at 3500× g for 10 minutes and the supernatant was collected for the determination of liver antioxidants with UV spectrophotometer using assay kits according to instruction manuals. The values for liver SOD, T-AOC, and GSH-Px were expressed in U/mg (U per milligram of protein), while liver MDA contents were interpreted in nmol/mg (nanomoles per milligram) [35,36].…”

Welfare of broilers on farm

“…Special focus is set on the deviation in the vertical axis of the legs, scored on live birds. Severe deviations will be reflected in the gait score. It includes valgus‐varus angulation (lateral or medial angulation of the shaft of the distal tibio‐tarsal bone) resulting in deviation of the lower part of the leg and frequently with bending of the proximal shaft of the tarsometatarsus (Julian, 1984; Guo et al., 2019; Jiang et al., 2019). The presence of leg deformation can lead to impaired welfare (Guo et al., 2019).…”

Chemical and Biological Characteristics of Anacardic acid as a Bioactive Compound and Its Application on Ruminant

“…Asam anakardat dilaporkan memiliki sifat antioksidan dan Kubo et al ( 2006) melakukan beberapa uji untuk mengamati mekanisme antioksidan pada asam anakardat. Sifat antioksidan yang dimiliki oleh asam anakardat bukan merupakan "radical scavenging" (menahan radikal bebas) tetapi antioksidan yang preventif karena menghambat beberapa enzim 'prooxidant" seperti xanthine oxidase, lipoxygenase dan mampu menekan malondialdehyde (MDA) (Jiang et al 2019). Selain itu, asam anakardat mampu membentuk khelat dengan zat besi yang dapat memicu oksidasi (Kubo et al 2006).…”