The aim of this research was to determine the nutrient content, digestibility on wafer of feed supplement and productivity of Bali calves. This research had two steps experimental, the first steps experiment to determine the nutrient content and digestibility on wafer of feed supplement. The composition wafer of feed supplement i.e. T1 = wafer containing lamtoro leaf, T2 = wafer containing lamtoro and papaya leaf, T3 = wafer containing moringa leaf, T4 = wafer containing gamal leaf, T5= wafer containing corn leaf and corn. Nutrient content and digestibility of T1 had highest compared among the others, i.e. crude protein (32.34%), ash (7.24%), crude fiber (16.85%), crude fat (4.52%), NFE (39.06 cal/g), digestibility of dry matter 82.87 %, digestibility of organic matter 81.78%, NH 3 9.33% and VFA 164.55%. The second steps of this research was the best result of the nutrient content and digestibility of wafer of feed supplements tested to bali calves. Level of wafer containing lamtoro leaf ,i.e R1 =0%, R2 =5%, R3 =10%, R4=15%. The result indicated that treatments had significant effect (P<0.05) on daily weight gain. It was concluded that bali calves were fed by wafer of feed supplement with level 10% had 69% higher than conventional.

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One of the strategies to reduce methane produced by ruminants is by the application of feed additive from plant materials. One of the potential plants is cashew plant especially its shell. The cashew nut shell can be processed to become 3 bioindustrial products; ie biofat, biochar, biosmoke. The aim of this research was to evaluate the effectivity of different levels of biofat, biochar and biosmoke against methane and other fermented products.This experiment was arranged in block randomised design. The treatments were: Control (substrate=S), Biofat: S+0.25 µLmL^-1, S+0.5 µLmL^-1, S+0.75 µLmL^-1; Biochar: S+0.1 mgmL^-1, S+0.2 mgmL^-1, S+0.3 mgmL^-1; Biosmoke: S + 2.5 µLmL^-1, S+5.0 µLmL^-1, S + level7.5 µLmL^-1. Each treatment was done in duplicates and the<em> in vitro</em> experiment was repeated 4 times. The measured variables were: total gas production, methane production, (dry matter, organic matter, NDF) degradability, ammonia concentration (NH₃) and partial VFA concentration. The results showed that biofat, biochar and biosmoke reduced methane productionas much as  43.88%, 24.21%, 37.88%  at the highest level of inclusion, respectively. NH₃ slightly increased by biochar and biosmoke addition compared to control. Molar proportion of acetic acid decreased and propionic acid increased by addition of cashew nut shell bioindustrial products compared to control. Organik matter degradability decreased significantly with biofat and biosmoke addition. Different mechanisms of biofat, biochar and biosmoke were proposed in affecting rumen fermentation. It can be concluded that each bioindustrial product of cashew nut shell (biofat, biochar and biosmoke) can be utilised as feed additive to reduce methane and increase propionic acid in the rumen fermentation.

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Biofat is a hexane extract containing several bioactive compounds with anacardic acid as the major compound. This study aimed to examine the effect of anacardic acid on rumen fermentation, especially methane and its degradation in the in vitro rumen fermentation. The study was arranged in a completely randomized block design. The treatments were control (substrate or complete feed), biofat (substrate + 0.75 uL/mL biofat), and anacardic acid (substrate + 0.75 uL/mL anacardic acid). Measured variables were total gas production, methane, pH, concentration of ammonia (NH 3 ), dry matter degrability (DMD), organic matter degrability (OMD), and neutral detergent fiber degrability (NDFD) in the rumen. The chromatogram GC-MS analysis results indicated that the anacardic acid isolation process of the biofat produced nearly pure isolate (99.44%), and significantly decreased the production of methane by 51.21% and 39.62%, respectively. Anacardic acid degradation pattern in the in vitro rumen test showed a shifting of retention factor (Rf) value after anacardic acid being incubated with the degradation of anacardic acid occurred after 24 h of fermentation. In conclusion, anacardic acid isolated from biofat has a dominant role to reduce the in vitro methane production. Anacardic acid is very potential to be used as a methane reducing agent. Keywords