Effect of anoxia and Polyscias filicifolia Bailey biomass tincture on the activity of tRNA and aminoacyl-tRNA synthetases in isolated pig heart

Kašauskas, Artūras; Rodovičius, Hiliaras; Vieželienė, Dalė; Lažauskas, Robertas

doi:10.3390/medicina45060064

Cited by 2 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the changes in cytosol have caused a major decrease in the rate and the level of protein synthesis. As reported earlier, a decrease in protein synthesis intensity under oxygen deprivation was related to the changes in the activity of aminoacyl-tRNA synthetases and tRNA methyltransferases (23), tRNA and aminoacyl-tRNA synthetases in the rabbit liver (8), and tRNA and aminoacyl-tRNA synthetases in the pig myocardium (7,17). The decrease in the rate and the level of protein synthesis in the cell-free translation system from the pig heart after 20 and 90 minutes of anoxia was similar to the decrease in the activity of tRNA and aminoacyl-tRNA synthetases under the same conditions.…”

Section: Discussionmentioning

confidence: 54%

“…As reported earlier, the preparations of Polyscias filicifolia Bailey biomass have a protective effect on the activity of tRNA and aminoacyl-tRNA synthetases in the rabbit liver under experimental myocardial infarction (19) and in the pig heart under anoxic perfusion (7,17). In the case of the 20-and 90-minute anoxic perfusion, the protective effect of the Polyscias filicifolia Bailey tincture on the protein synthesis process was analogous to the protective effect on the activity of tRNA and aminoacyl-tRNA synthetases.…”

Section: Discussionmentioning

confidence: 59%

“…It is known that the activity of tRNA in the pig heart is regulated by the formation of inactive conformers under anoxic conditions. The activity of aminoacyl-tRNA synthetases is regulated by changing the activity of inorganic pyrophosphatase under anoxia (17). The use of the Polyscias filicifolia Bailey biomass tincture can protect the heart from these changes.…”

Section: Discussionmentioning

confidence: 99%

“…The lack of oxygen influences protein synthesis at different levels of this process. It may be associated with the rate of energy production (5), RNA transcription regulation (6), aminoacyl-tRNA formation (7,8), and regulation of mRNA translation (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

“…One of them is Polyscias filicifolia Bailey. Polyscias filicifolia Bailey biomass, in the same way as the Panax ginseng C. A. Mey root, contains triterpenic saponins and protects from stress reactions and disturbances of oxygen deprivation (7,14,(16)(17)(18)(19). It is worth noting that the alcohol extract of Polyscias filicifolia biomass does not show cytotoxic and genotoxic activity.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Effect of Polyscias filicifolia Bailey Biomass Tincture on the Protein Synthesis Process in the Heterogeneous System From the Isolated Pig Heart

Kašauskas

Mongirdienė

2013

Medicina

Self Cite

View full text Add to dashboard Cite

Background and Objective. An insufficient supply of oxygen to the heart influences the process of protein synthesis. The aim of this study was to determine the effect of the Polyscias filicifolia Bailey biomass tincture on the protein synthesis process in a heterogeneous translation system from the isolated pig heart.Materials and Methods. The effect of anoxia was evaluated after 20-and 90-minute anoxia. With the aim to determine the effect of Polyscias, the pig hearts were perfused with a buffer containing the Polyscias filicifolia Bailey biomass tincture. To determine the rate and the level of translation, the incorporation of [ 14 C]-leucine into translational products in a cell-free system was measured.Results. The protein synthesis level decreased by 23%-42% when the translation system containing cytosol from the anoxic heart was used. When the translation system containing a ribosomal fraction after 20-minutes anoxia was used, the protein synthesis level was the same as in the control. In the case of 90-minute anoxia, it decreased by 16%. The protein synthesis rate and the level in the translation system containing cytosol from the heart after 20-minute anoxic perfusion with the buffer containing Polyscias was the same as in the control.Conclusions. A decrease in the protein synthesis rate and the level after 20-minute anoxia was determined by changes in cytosol. On the other hand, 90-minute anoxia caused changes in cytosol and the ribosomal fraction. The Polyscias filicifolia Bailey biomass tincture restored the protein synthesis process acting on the components of the translation system in cytosol and the ribosomal fraction.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Effect of Polyscias filicifolia Bailey Biomass Tincture on the Protein Synthesis Process in the Heterogeneous System From the Isolated Pig Heart

Kašauskas

Mongirdienė

2013

Medicina

Self Cite

View full text Add to dashboard Cite

show abstract

Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

et al. 2018

View full text Add to dashboard Cite

In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of Polyscias filicifolia was developed. The phenolic compound content in P. filicifolia has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of P. filicifolia extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.

show abstract

Effect of anoxia and Polyscias filicifolia Bailey biomass tincture on the activity of tRNA and aminoacyl-tRNA synthetases in isolated pig heart

Cited by 2 publications

References 20 publications

The Effect of Polyscias filicifolia Bailey Biomass Tincture on the Protein Synthesis Process in the Heterogeneous System From the Isolated Pig Heart

The Effect of Polyscias filicifolia Bailey Biomass Tincture on the Protein Synthesis Process in the Heterogeneous System From the Isolated Pig Heart

Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

Contact Info

Product

Resources

About