Effect of antagonistic yeast Pichia membranaefaciens on black spot decay of postharvest broccoli

Guo, Huicai; Cui, Yonghao; Zhu, Enlong; Zhang, Song; Wang, Chengxin

doi:10.1007/s10658-015-0689-5

Cited by 9 publications

(2 citation statements)

References 40 publications

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“…P. anomala AR 2016 contains 59.56% crude protein and various amino acids, with a high content of aspartic acid, glutamate, and glycine [ 12 ]. AR 2016 produces a killer toxin (protein or glycoprotein toxin) that can be used to control harmful microorganisms in animals, plants, feed grains, and fermentation cultures [ 17 , 18 ]. A killer toxin provided by P. anomala AR 2016 has no harmful effects on piglets [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Identification and Function of Pichia anomala AR2016 and Its Effects on the Growth and Health of Weaned Pigs

Sun

Zeng

et al. 2021

Animals

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This study isolates and identifies Pichia anomala (P. anomala) AR2016, and studies its effect on the growth and health of weaned pigs. A P. anomala strain from solid wine koji is isolated and identified using 26S rDNA analysis, and its culture conditions are optimized. Heat tolerance, bile salt tolerance, artificial gastric, and intestinal juice tolerance are evaluated. In our methodology, thirty 28 d Large White × Landrace × Rongchang weaned pigs were randomly divided into three groups with 10 barrows in each, and fed a maize-soybean meal diet and orally administered 0.85% saline (CK), 1 mL 1 × 109 cfu/mL Candida utilis (C. utilis), and 1 mL 1 × 109 cfu/mL P. anomala once daily for 28 days. A P. anomala strain was identified and named P. anomala AR2016. P. anomala AR2016 grew best in yeast extract peptone dextrose medium with pH 5.0 at 28 °C, 180 r/min and could tolerate 45 °C for 0.5 h, 0.2% pig bile salts, simulated gastric fluid, and 1.0% simulated intestinal fluid. Our results show that compared with the CK group, orally administered P. anomala AR2016 increases average daily gain, the ileal villus height, the ileal mucosal concentrations of occludin and zonula occluens-1, the serum glucose and total protein concentration, total superoxide dismutase, glutathione peroxidase, and total antioxidative capacity activity, the trypsin and lipase activity in jejunal and ileal contents, the jejunal and ileal mucosa mRNA levels of ALP, TNF-α, and TLR-2, and the relative abundance of Bacteroidetes, Actinobacteria, Succinivibrionaceae, Lachnospiraceae, and Prevotellaceae (p < 0.05). Compared with the CK group, oral administration of P. anomala AR2016 decreased the incidence of diarrhea, aspartate aminotransferase activity, alanine amino-transferase-activity, malondialdehyde, D-lactic acid and endotoxin content in serum, the mRNA level of aminopeptidase N of ileum mucosa, and the relative abundance of Proteobacteria, Clostridiaceae, Campylobacteraceae, Vibrionaceae, Bacillus, and Pseudon (p < 0.05). Collectively, the study indicates that P. anomala AR2016 can tolerate high acidity and high bile salts, and has high survivability in the artificial gastric intestinal juice environment. Oral administration of P. anomala AR2016 improves the growth performance, reduces the incidence of diarrhea, enhances intestinal barrier function, and improves microflora in weaned pigs.

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Section: Discussionmentioning

confidence: 99%

Isolation, Identification and Function of Pichia anomala AR2016 and Its Effects on the Growth and Health of Weaned Pigs

Sun

Zeng

et al. 2021

Animals

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“…Cellulase activity was determined by the absorbance of a standard D-glucose solution (Guo, Cui, Zhu, Song, & Wang, 2015;Hashem & Alamri, 2009). Cellulase activity in tissue-free filtrate was expressed as mg reducing sugars per ml in each hour.…”

Section: Cellulase Activitymentioning

confidence: 99%

Effectiveness of preharvest application of submicron chitosan dispersions for controlling Alternaria rot in postharvest jujube fruit

Guo

Xing

et al. 2017

Journal of Phytopathology

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The efficacy of submicron chitosan dispersions (SCD) on Alternaria alternata rot of the ‘Lingwu changzao’ jujube (Zizyphus jujuba Mill. cv. Lingwu changzao) fruit and possible mechanisms involved were investigated in this study. When jujube fruit at the preharvest stage were sprayed twice with SCD (10 mg/ml) water solution and then natural infections were allowed to develop, there was a significant reduction in disease incidence and lesion index compared to control fruit during storage period, and similar results were obtained from postharvest fruit inoculated with the Alternaria rot pathogen. Scanning electron microscopy of the pathogen revealed that hyphae and spores were damaged and deformed when growing on the surface of fruit pretreated with SCD or CHO. The slowed softening of jujube fruit resulted from the protopectin content increasing compared with the control. Furthermore, SCD sprays inhibited cell wall hydrolysing enzymes, pectinase activity was decreased, and the increase in cellulase activity was postponed (from 30 days to 45 days).It was concluded that preharvest application of SCD was a more effective alternative to conventional chitosan for preventing postharvest from Alternaria rot of ‘Lingwu changzao’ jujube fruit.

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Yarrowia lipolytica reduces the disease incidence of asparagus infected by Fusarium proliferatum by affecting respiratory metabolism and energy status

Zhang

Godana

et al. 2021

Biological Control

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Effect of antagonistic yeast Pichia membranaefaciens on black spot decay of postharvest broccoli

Cited by 9 publications

References 40 publications

Isolation, Identification and Function of Pichia anomala AR2016 and Its Effects on the Growth and Health of Weaned Pigs

Isolation, Identification and Function of Pichia anomala AR2016 and Its Effects on the Growth and Health of Weaned Pigs

Effectiveness of preharvest application of submicron chitosan dispersions for controlling Alternaria rot in postharvest jujube fruit

Yarrowia lipolytica reduces the disease incidence of asparagus infected by Fusarium proliferatum by affecting respiratory metabolism and energy status

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