Effect of Anti-Müllerian hormone (AMH) and bone morphogenetic protein 15 (BMP-15) on steroidogenesis in primary-cultured human luteinizing granulosa cells through Smad5 signalling

Prapa, Ermioni; Vasilaki, Anna; Dafopoulos, Konstantinos; Katsiani, Eleni; Georgoulias, Panagiotis; Messini, Christina I.; Anifandis, George; Messinis, Ioannis E.

doi:10.1007/s10815-015-0494-2

Cited by 41 publications

(26 citation statements)

References 59 publications

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“…Another explanation for the presence of AMH in luteal cells may be that it exhibits a role in the local regulation of progesterone production in the corpus luteum. This assumption is supported by the study of Prapa et al [ 45 ], who reported that AMH exposure of luteinized human granulosa cells in vitro, decreased basal progesterone production. AMH is a member of the transforming growth factor B (TGFB) superfamily, that further includes bone morphogenetic proteins (BMPs), activins, inhibins and growth and differentiation factor 9 (GDF9).…”

Section: Discussionsupporting

confidence: 54%

Presence of anti-Müllerian hormone (AMH) during follicular development in the porcine ovary

et al. 2018

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BackgroundAnti-Müllerian hormone (AMH) is expressed by granulosa cells of developing follicles and plays an inhibiting role in the cyclic process of follicular recruitment by determining follicle-stimulating hormone threshold levels. Knowledge of AMH expression in the porcine ovary is important to understand the reproductive efficiency in female pigs.Research aimIn the present study we investigated the expression of AMH during follicular development in prepubertal and adult female pigs by immunohistochemistry, laser capture micro-dissection and RT-qPCR.Results and conclusionAlthough in many aspects the immunohistochemical localization of AMH in the porcine ovary does not differ from other species, there are also some striking differences. As in most species, AMH appears for the first time during porcine follicular development in the fusiform granulosa cells of recruited primordial follicles and continues to be present in granulosa cells up to the antral stage. By the time follicles reach the pre-ovulatory stage, AMH staining intensity increases significantly, and both protein and gene expression is not restricted to granulosa cells; theca cells now also express AMH. AMH continues to be expressed after ovulation in the luteal cells of the corpus luteum, a phenomenon unique to the porcine ovary. The physiological function of AMH in the corpus luteum is at present not clear. One can speculate that it may contribute to the regulation of the cyclic recruitment of small antral follicles. By avoiding premature exhaustion of the ovarian follicular reserve, AMH may contribute to optimization of reproductive performance in female pigs.

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Section: Discussionsupporting

confidence: 54%

Presence of anti-Müllerian hormone (AMH) during follicular development in the porcine ovary

et al. 2018

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“…Reduction of estradiol concentration over the controls and BMP15 treated groups on day 21 maybe due to the differentiation of these cells. Our observations correspond to Prapa et al (2015) who found that BMP15 could reduce basal estradiol levels in human granulosa cells; however, Costa et al (2004) showed that levels of estradiol considerably decreased in the follicles with mature oocytes compared to the follicles with immature oocytes. We conclude that FF derived MSCs are a structural portion of the ovarian tissue, whether from OSE or from the cortical zone of the ovary.…”

Section: Discussionsupporting

confidence: 91%

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Supplemental Information 24: Cytoplasmic Immunostaining for vimentin.Raw Data

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Background. To study the effect of Bone morphogenetic protein 15 on differentiation potential of mesenchymal stem cell derived from human follicular fluid to oocyte like cell. Methods. Human FF derived cells were collected from 78 women in assisted fertilization program, and cultured in differentiation medium containing human recombinant BMP15 for 21 days. Mesenchymal stem cells and OLCs were characterized by real-time PCR and immunocytochemistry (ICC) staining. Results. MSCs expressed germ line stem cell markers, such as OCT4 and NANOG. After 15 days, OLCs formed and expressed zona pellucida markers (ZP2, ZP3), and reached 20-30 µm in diameters. Ten days after induction with BMP15, round cells remarkably developed, and the maximum size of OLCs reached 115 µm. Finally, a decrease ranging from 0.04 to 4.5 in the expression of pluripotency and oocyte specific markers was observed in the cells cultured in BMP15 supplemented medium. Our work demonstrates, FF derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in stimulating the differentiation of these cells, which may give an in vitro model to examine human germ cell development.

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“…AMH is produced by the ovarian granulosa cells and supports the follicular growth [4]. Effect of AMH and BMP-15 on estradiol and progesteron production in primarycultured human luteinizing granulosa cells was reported [5]. BMP-15 level in follicular fluid has been demonstrated to associate with oocyte and embryo quality [6].…”

Section: Introductionmentioning

confidence: 99%

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Ilhan¹,

Özkan²,

Ekinci³

et al. 2017

Histol Cytol Embryol

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Objective: Bone morphogenetic protein-15 (BMP-15) and anti-Müllerian hormone (AMH) are the proteins functioning during ovarian follicular growth. We aimed to investigate the impact of serum and follicular fluid (FF) AMH and BMP-15 levels on outcomes of intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles.Methods: This prospective, cross-sectional study was carried out with 82 patients attending ICSI-ET cycle. The women divided into two groups according to using either GnRH agonist (group1, N=38) or antagonist (group2, N=44) for pituitary down-regulation. Eight milliliter of serum samples were taken from the patients on the 3rd day of menstrual period (D3) and on the day of oocyte pick-up (OPU); and 8 mL of follicular fluid (FF) sample was collected during OPU. AMH and BMP-15 measurements were carried with ELISA method quantitatively. Results:The mean age and body mass index of population were 30.6 ± 5.2 years and 25.5±4.2 kg/m 2 respectively. The comparison of pregnancy rates between women according to having FF AMH (35% vs 42%) and FF BMP-15 (37.8% vs 40.5%) levels under and above of the mean values revealed no significant difference. Serum AMH levels showed negative correlation with fertilization rate (FR). D3 BMP-15 level showed positive correlation with progesterone level and endometrial thickness on the day of hCG injection.Conclusions: AMH and BMP-15 monitoring during ICSI-ET cycles for predicting cycle outcomes should be evaluated with further studies.

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Effect of Anti-Müllerian hormone (AMH) and bone morphogenetic protein 15 (BMP-15) on steroidogenesis in primary-cultured human luteinizing granulosa cells through Smad5 signalling

Abstract: AMH and BMP-15 by interacting with FSH affect the production of estradiol and progesterone from cultured luteinizing-granulosa cells possibly via Smad5-protein phosphorylation.

Cited by 41 publications

References 59 publications

Presence of anti-Müllerian hormone (AMH) during follicular development in the porcine ovary

Presence of anti-Müllerian hormone (AMH) during follicular development in the porcine ovary

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What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

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