Effect of Anti-stress Activity of Fluoxetine on Restrained Stress Induced Male Albino Rats in Hematological Parameters and Whole Brain Histopathology

Abstract

Objective: In this study we investigated the effect of Fluoxetine, a selective serotonin reuptake inhibitor, on the gravimetry, hematology and whole brain histopathology in the restrained stressed male albino rats. Methodology: Adult male Wistar albino rats weighing about 175-225 g were taken for the study and were divided into four groups of six animals each. Group I (control), Group II (stress induced), Group III (stress + withdrawal) and Group IV (stress + Fluoxetine, 20 mg/kg body weight, i.p.). The gravimetrical parameters, hematological parameters and whole brain histopathology of all the experimental rats were evaluated. Results: After 42 days of restraint stress, there was a significant (P ≤0.05) decrease final body weight, whole brain weight, Hb, RBC, MCV, MCH, total WBC and Platelets, whereas increase in Neutrophils, lymphocytes, eosinophils and monocytes in Group II of restraint stress rats when compared with their control group I. The stress withdrawal group (Group III) and drug Fluoxetine treatment (Group IV) shown significantly (P≤0.05) improvement in gravimetry, hematological parameters and whole brain histopathology in restraint stress compared to only stress induced group II. Conclusion: The drug Fluoxetine treatment could exert a protective effect on restrained stress induced alterations in gravimetrical parameters, hematological parameters and whole brain histopathology of male albino rats.

Ethanolic extract of Ocimum Gratissimum (Scent Leaf) leaves improves haematological parameters in restrain-stressed rats

“…It has the ability of interrupting the functions of neuroendocrine cells and releasing hormones of the hypothalamus (O'Connor et al, 2020). Consequently, hematological parameters have been proven as viable markers for the assessment of health in humans and animals and several studies have focused on hematological indices as an indicator for stress evaluation (Kandeepan, 2014;Kori et al, 2017, Pandian et al, 2018Ueno et al, 2019;Bamidele et al, 2020). Results from our study recorded an increase in PCV and total RBC in the stressed groups.…”

“…However, following administration with OG extract, we recorded a dose-dependent significant increase in PCV and total RBC compared to vitamin C that is a standard antioxidant. This implies that extract of OG alters stress-induced polycythemia and erythrocytosis and confirms that OG leaf extract is more potent in alleviating stress than vitamin C. The decline might be linked to the antioxidative activity of OG through its total phenolic content (Ofem et al, 2012;Kori et al, 2017;Okechukwu et al, 2019). Reasons for this observation may be attributed to the fact that tissue anoxia causes the production of erythropoietin, which enhances erythrocyte production (Bowman et al, 2009).…”

Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure

“…Consistent with the research of Osman et al [78] , pollution might have stimulated an increase in the number of immature red blood cells in the fish circulation. Furthermore, stress can also affect the release of immature red blood cells [79] . Importantly, immature erythrocytes and erythrocyte deformability can affect their inefficient functions, such as inadequate blood flow and reduced oxygen transport to the tissues of organs within the organism [75] .…”

Protective role of hispolon pyrazole and hispolon monomethyl ether pyrazole in electromagnetic radiation-induced behavioral, neurochemical, oxidative, and histological changes in rats

“…Ltd. 's PG/FB series was used to measure the animal's body weight before exposure to mobile phone radiation and after every 30 days until the end of the experiment. The % change in the body weight was measured using the following equation (36).…”