Effect of antibiotic prophylaxis on Coagulase-negative Staphylococcus virulence factor profiles in patients undergoing cataract surgery

López, Yolanda; Samudio, Margarita; Fariña, Norma; Castillo, Verónica; Abente, Sonia; Nentwich, Martin M.; González-Brítez, Nilsa; Laspina, Florentina; Carron, A.; Cibils, D; Kaspar, Herminia Miño de

doi:10.1007/s10792-016-0355-8

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…[28] found a large percentage of methicillin-resistant commensal CNSs carrying the ica and atlE genes. The results of the present study are in agreement with previous work carried out by our group in which a high percentage of the ocular microbiota of patients undergoing cataract surgery carries encoding virulence factor genes [11][12][13]. In addition, we have shown a signi cant percentage of patients showed conjunctival isolates before surgery that recovered again after disinfection with 5% povidone-iodine or at the end of surgery.…”

Section: Discussionsupporting

confidence: 93%

“…Our group has shown in previous studies a high contamination rate of the conjunctiva before and after irrigation with 5% PVI and at the end of the surgery [11,12]; as well as the aqueous humor [7]. In addition, a high frequency of virulence factors in strains isolated from the conjunctiva in patients undergoing cataract surgery [13], and from the health personnel [14] were reported. In none of these studies, the source of colonization was established.…”

Section: Introductionmentioning

confidence: 98%

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Persistence of conjunctival colonization by coagulase-negative staphylococci in patients undergoing cataract surgery

Samudio

Abente

Fariña

et al. 2022

Preprint

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PurposeTo establish the conjunctival contamination source by coagulase-negative staphylococci (CNS) during cataract surgery and discuss the implications of this colonization as a potential risk of acquiring endophthalmitis.Methods Conjunctiva samples for culture from 59 patients undergoing cataract surgery were taken of the fellow eye at baseline (T1) and from the eye to be operated before (T0) and immediately after (T2) irrigation with povine-iodine IOP5%, and at the end of surgery (T3). Genes responsible for virulence (mecA, ica and atlE) and antibiotic profile were determined; strain clonality of persistent colonizing S. epidermidis strains was established by the Multi-locus sequence typing (MLST).Results The frequency of CNS was significantly reduced in T2 (13.6%) from 81.4% in T0 and 86.4% in T1. The frequency of mecA, ica and atlE genes was 35.9%, 41.7% and 67.2%, respectively; and methicillin phenotypic resistance was 35.4%. S. epidermidis was the most frequent species isolated in every time point. MLST revealed in 7 patients 100% coincidence of the 7 alleles of the S. epidermidis isolated previous to povine-iodine 5% disinfection and at the end of the surgery. CNS isolates from T2 or T3 corresponded to the same species, antibiotic and virulence profile as those isolates from T1 or T0.Conclusion Pre-surgical prophylactic scheme significantly reduced conjunctival contamination; in those that persisted, the source of contamination was mostly the patient's microbiota confirmed by the MLST system.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 98%

Persistence of conjunctival colonization by coagulase-negative staphylococci in patients undergoing cataract surgery

Samudio

Abente

Fariña

et al. 2022

Preprint

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“…Another study by López et al . [ 20 ] could not establish any additional benefits of topical moxifloxacin with regard to reduction in conjunctival flora when compared with PVI 5%.…”

Section: Discussionmentioning

confidence: 99%

Impact of topical moxifloxacin prophylaxis and povidone iodine on conjunctival bacterial flora in patients receiving intravitreal injections in a tertiary healthcare center in India

Sahu

Priya

Aggarwal

2022

Journal of Family Medicine and Primary Care

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Purpose: To assess the efficacy of 3 days of topical moxifloxacin in combination with povidone–iodine (PVI) versus moxifloxacin/PVI alone in eliminating conjunctival bacterial flora in patients scheduled to undergo intravitreal injections (IVI). Patients and Methods: A prospective randomized comparative study in which 120 patients scheduled to undergo IVI at a tertiary care hospital in New Delhi were selected. Study patients were randomized into two groups. Both the groups received self-administration of moxifloxacin for 3 days prior to injection, except in Group B where it was preceded by PVI. Cultures were obtained at different time intervals, such as in Group A before and after applying moxifloxacin (for 3 days) and once again after applying PVI just before the procedure in the operating room, whereas in Group B first two samples were taken before and after applying PVI and the last sample was taken on the day of the procedure after 3 days of moxifloxacin application. Results: A statistical significance was seen between moxifloxacin prophylaxis and resistant coagulase-negative Staphylococcus (CoNS) ( P = 0.0001), which implies that frequent use of antibiotic prophylaxis will ultimately lead to the formation of resistant organisms in the conjunctival flora, especially when repeated IVI are given. Conclusion: We could not establish any additional benefits of topical moxifloxacin prophylaxis with regard to a reduction in conjunctival flora when compared with PVI 5%. PVI can be used as an efficient monotherapy in patients undergoing repeated IVI.

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“…Previous research conducted by the authors involved assessing biofilm production through phenotypic methods in Coagulase-negative Staphylococci (CoNS) isolates (14). This work revealed a high frequency of genes associated with virulence factors related to biofilm formation (ica and atlE), as well as the mecA gene, identified through a multiplex PCR assay, in coagulase-negative Staphylococcus isolates obtained from ocular samples of patients undergoing cataract surgery (15,16).…”

Section: Original Introductionmentioning

confidence: 99%

Comparative Study of Staphylococcus epidermidis Virulence Profiles in an animal Model of Endophthalmitis

Samudio,

Abente,

Villalba

et al. 2024

Med Clín Soc

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Introduction: Gram-positive staphylococci are responsible for over 90% of cases of postoperative infectious endophthalmitis, with Staphylococcus epidermidis accounting for approximately 70% of isolated microorganisms. Objective: To evaluate the relationship between S. epidermidis virulence and severity of endophthalmitis in an animal model. Methodology: New Zealand albino rabbits were divided into two groups and administered 100 colony-forming units of S. epidermidis strains. In the virulent group, four rabbits received a multi-antibiotic resistant, mecA, ica, and atlE gene carrying S. epidermidis strain, from a patient´s conjuntival microbiota and three a biofilm-forming S. epidermidis ATCC 35984. In the non-virulent group, five rabbits were inoculated with a strain sensitive to all tested antibiotics and lacking mecA, ica, and atlE genes, also from a patient and three rabbits received the non-producer biofilm S. epidermidis ATCC 29122. Clinical and ultrasound examinations were conducted every three hours until endophthalmitis symptoms appeared, followed by daily clinical assessments. Histological evaluations were performed 15 days post-inoculation. Results: The less virulent group displayed milder inflammation and reduced intraocular damage in comparison to the more virulent group based on clinical and ultrasound observations. Nevertheless, histopathological analysis revealed similar inflammation in all groups, 15 days post-inoculation. Discussion: Less virulent S. epidermidis strains induced less severe inflammation as observed through clinical and ultrasound assessments. However, long-term histopathological assessments showed effects comparable to those seen with the more virulent strain.

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Effect of antibiotic prophylaxis on Coagulase-negative Staphylococcus virulence factor profiles in patients undergoing cataract surgery

Abstract: A high frequency of genes encoding virulence factors was observed in the coagulase-negative Staphylococcus isolates. The use of moxifloxacin did not significantly modify the CNS virulence factor profiles.

Cited by 5 publications

References 41 publications

Persistence of conjunctival colonization by coagulase-negative staphylococci in patients undergoing cataract surgery

Persistence of conjunctival colonization by coagulase-negative staphylococci in patients undergoing cataract surgery

Impact of topical moxifloxacin prophylaxis and povidone iodine on conjunctival bacterial flora in patients receiving intravitreal injections in a tertiary healthcare center in India

Comparative Study of Staphylococcus epidermidis Virulence Profiles in an animal Model of Endophthalmitis

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