Effect of antigen and octylamine on mast cells and histamine content of sensitized guinea‐pig tissues

Mota, I.

doi:10.1113/jphysiol.1959.sp006253

Cited by 68 publications

(19 citation statements)

References 20 publications

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“…It should be emphasized that our findings are based on measurements of histamine release and are thus strictly speaking relevant only to the binding of antibody to histamine-releasing cells, probably mast cells (Mota, 1959). It is possible that only mast cells exhibit specificity in globulin uptake, since Humphrey & Mota (1959) have shown that the gross tissue uptake of y-globulins does not depend on their sensitizing properties.…”

Section: Passive Sensitization Discussionmentioning

confidence: 99%

A study of the mechanism of passive sensitization

Mongar¹,

Schild²

1960

The Journal of Physiology

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Section: Passive Sensitization Discussionmentioning

confidence: 99%

A study of the mechanism of passive sensitization

Mongar¹,

Schild²

1960

The Journal of Physiology

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“…Computer-aided analysis of mast cell metachromasia Specimens of the right and left guinea-pig auricles and of rat ileum were fixed according to the procedure described by Mota (1959) and Enerback (1966) Wereld, 1981).…”

Section: Assay Of Histaminementioning

confidence: 99%

The release of histamine by parasympathetic stimulation in guinea‐pig auricle and rat ileum.

Bani-Sacchi¹,

Barattini²,

Bianchi³

et al. 1986

The Journal of Physiology

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SUMMARY1. Two preparations, a segment of rat ileum and the vagally innerved guinea-pig auricles, have been used in an analysis of the responses to vagal or to electrical field stimulation.2. The responses to parasympathetic stimulation were depressed by atropine and by tetrodotoxin, and potentiated by eserine.3. Supramaximal stimulation (10-20 Hz) resulted in increased release of acetylcholine and histamine, both in rat ileum and guinea-pig auricles.4. The release of histamine after parasympathetic stimulation did not exhibit tachyphylaxis, and it was not reproduced by non-parasympathetic stimuli.5. In both preparations, atropine produced a significant, dose-related reduction of histamine measured in the bath fluid after stimulation, while eserine increased histamine output.6. A significant diminution of mast cell granules metachromasia was observed in guinea-pig auricles and in rat intestine after parasympathetic stimulation.7. The possibility is discussed that acetylcholine released by parasympathetic stimulation would in turn evoke the secretion of histamine from tissue mast cells.

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“…In most sensitized tissues a decrease in mast cell content also accompanies the manifestations of anaphylaxis (Mota & Vugman, 1956 ;Mota, 1959), but this has not appeared to be so with the ileum (Boreus & Chakravarty, 1960).…”

Section: Introductionmentioning

confidence: 99%

The Schultz‐Dale response of the longitudinal muscle strip preparation of guinea‐pig ileum

Dale

Zilletti

1970

British J Pharmacology

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Summary1. The mast-cell distribution in the various layers of the ileum has been described.2. Histamine-content, anaphylactic histamine-release and the anaphylactic dose-response curve of the full-thickness ileum and of the longitudinal muscle strip have been measured and compared. 3. Tetrodotoxin 10-7 g/ml had no obvious effect on the anaphylactic doseresponse curve of either preparation. This suggests that the plexus is not of any great importance in the Schultz-Dale reaction. 4. Exposure of the longitudinal muscle strip to octylamine 10-3 g/ml for 1 min reduced the mast cell content by 95-100%. After this treatment the dose-response curve to antigen was eliminated, although the muscle still responded to small doses of histamine and to anaphylactic mediators. Pretreatment of antibody with octylamine did not impair passive sensitization and subsequent response to antigenic challenge. This suggests that the classical Schultz-Dale reaction in the strip is mediated mainly by mast cells, and possibly other cells, and is probably not due to a direct effect of the antigen-antibody reaction on the smooth muscle. 5. The typical three-phase anaphylactic response (quick contraction, quick relaxation, slow contraction) of full-thickness ileum is discussed and com-

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Effect of antigen and octylamine on mast cells and histamine content of sensitized guinea‐pig tissues

Cited by 68 publications

References 20 publications

A study of the mechanism of passive sensitization

A study of the mechanism of passive sensitization

The release of histamine by parasympathetic stimulation in guinea‐pig auricle and rat ileum.

The Schultz‐Dale response of the longitudinal muscle strip preparation of guinea‐pig ileum

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