Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats

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102

Allium saralicum R.M. Fritsch has been used in Iranian traditional medicine as a remedial supplement for microbial diseases. This paper reports the green synthesis, chemical characterization and antioxidant, cytotoxic, antibacterial and antifungal properties of silver nanoparticles obtained using aqueous extract of A. saralicum leaves. In this synthesis, no surfactants or stabilizers were used. For characterization, UV–visible spectroscopy, transmission electron microscopy, X‐ray diffraction, energy‐dispersive X‐ray spectroscopy and field emission scanning electron microscopy were used. 2,2‐Diphenyl‐1‐picrylhydrazyl was used in experiments to assess the antioxidant potential of the silver nanoparticles, which revealed an impressive prevention in comparison with butylated hydroxytoluene. The synthesized silver nanoparticles at low doses (1–250 μg dl−1) did not show marked cytotoxic activity (against cervical cancer cells (Hela), breast cancer cells (MCF‐7) and human embryonic kidney cells (HEK‐293)). Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. Compared with all standard antimicrobials, the silver nanoparticles showed higher antibacterial and antifungal activities (p ≤ 0.01). Also, the silver nanoparticles inhibited the growth of all bacteria and fungi at concentrations of 31–250 μg ml−1, and destroyed them at concentrations of 31–500 μg ml−1 (p ≤ 0.01). Because the silver nanoparticles obtained using aqueous extract of A. saralicum leaves have antioxidant, non‐cytotoxic, antifungal and antibacterial potentials, they can be used as a medical supplement or drug.

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Green synthesis and chemical characterization of silver nanoparticles obtained using Allium saralicum aqueous extract and survey of in vitro antioxidant, cytotoxic, antibacterial and antifungal properties

Zangeneh

Bovandi

Gharehyakheh

et al. 2019

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102

“…Iran has experimental plants that are widely distributed throughout the country, particularly in Kermanshah province, west of Iran (geographical coordinates: 34.3277°N and 47.0778°E) . They have been the foundation for inhibition and treatment of experimental diseases . One of the most important herbal medicines that is widely used is Ziziphora clinopodioides Lam .…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization, and assessment of cytotoxicity, antioxidant, antibacterial, antifungal, and cutaneous wound healing properties of titanium nanoparticles using aqueous extract of Ziziphora clinopodioides Lam leaves

Seydi

Mahdavi

Paydarfard

et al. 2019

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In recent decades, nanotechnology is growing rapidly owing to its widespread application in science and industry. The aim of the experiment was chemical characterization and evaluation of cytotoxicity, antioxidant, antibacterial, antifungal, and cutaneous wound healing activities of titanium nanoparticles using aqueous extract of Ziziphora clinopodioides Lam leaves (TiNPs@Ziziphora). These nanoparticles were characterized by fourier transformed infrared spectroscopy (FT‐IR), field emission scanning electron microscopy (FE‐SEM), energy dispersive X‐ray spectroscopy (EDS), and UV–visible spectroscopy. The synthesized TiNPs@Ziziphora had great cell viability dose‐dependently (Investigating the effect of the plant on human umbilical vein endothelial cells (HUVECs) cell line) and revealed this method was nontoxic. Then, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging test was done to assess the antioxidant properties, which indicated similar antioxidant potentials for TiNPs@Ziziphora and butylated hydroxytoluene. Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) were specified by macro‐broth dilution assay. The data were analyzed by SPSS 21 software (Duncan post‐hoc test). TiNPs@Ziziphora indicated higher antibacterial and antifungal effects than all standard antibiotics (p ≤ 0.01). Also, TiNPs@Ziziphora inhibited the growth of all bacteria at 2‐16 mg/ml concentrations and removed them at 2‐32 mg/ml concentrations (p ≤ 0.01). In case of antifungal properties of TiNPs@Ziziphora, they prevented the growth of all fungi at 2‐8 mg/ml concentrations and destroyed them at 2‐16 mg/ml concentrations (p ≤ 0.01). In vivo experiment, after creating the cutaneous wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 0.2% TiO2 ointment, treatment with 0.2% Z. clinopodioides ointment, and treatment with 0.2% TiNPs@Ziziphora ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at day 10. Use of TiNPs@Ziziphora ointment in the treatment groups substantially reduced (p ≤ 0.01) the wound area, total cells, neutrophil, and lymphocyte and remarkably raised (p ≤ 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate compared to other groups. In conclusion, the results revealed the useful non‐cytotoxic, antioxidant, antibacterial, antifungal, and cutaneous wound healing effects of TiNPs@Ziziphora.

“…Iran has experimental plants that are widely distributed throughout the country, particularly in Kermanshah province, western Iran (34.3277° N and 47.0778° E) . They have been the foundation for inhibition and treatment of experimental diseases . One of the most important herbal medicines that is widely used is Falcaria vulgaris .…”

Section: Introductionmentioning

confidence: 99%

Green synthesis and chemical characterization of silver nanoparticles from aqueous extract of Falcaria vulgaris leaves and assessment of their cytotoxicity and antioxidant, antibacterial, antifungal and cutaneous wound healing properties

Zangeneh

2019

Self Cite

Nanotechnology encompasses the understanding of the fundamental physics, biology, chemistry and technology of nanometre‐scale objects. In recent decades, nanotechnology has grown rapidly owing to its widespread application in science and industry. The aim of the work reported was the green synthesis and chemical characterization of silver nanoparticles from aqueous extract of Falcaria vulgaris leaves (AgNPs@Falcaria) and evaluation of their cytotoxicity and antioxidant, antibacterial, antifungal and cutaneous wound healing effects under in vitro and in vivo conditions. These nanoparticles were characterized using Fourier transform infrared and UV–visible spectroscopies, scanning and transmission electron microscopies and atomic force microscopy. 2,2‐Diphenyl‐1‐picrylhydrazyl free radical scavenging experiments were conducted to evaluate the antioxidant potential, which indicated similar antioxidant potentials for AgNPs@Falcaria and butylated hydroxytoluene. The synthesized AgNPs@Falcaria had great cell viability dose‐dependently, indicating their non‐toxicity. Minimum inhibitory, minimum bactericidal and minimum fungicidal concentrations were determined by macro‐broth dilution assay. The data were analysed using SPSS software (Duncan post hoc test). AgNPs@Falcaria revealed higher antibacterial and antifungal activities than many standard antibiotics (p ≤ 0.01). Also, AgNPs@Falcaria prevented the growth of all bacteria at 2–8 mg ml−1 concentrations and removed them at 4–16 mg ml−1 concentrations (p ≤ 0.01). AgNPs@Falcaria inhibited the growth of all fungi at 2–4 mg ml−1 concentrations and destroyed them at 4–8 mg ml−1 concentrations (p ≤ 0.01). For in vivo experiments, after creating a cutaneous wound, rats were randomly divided into six groups: untreated control, treatment with eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 0.2% AgNO3 ointment, treatment with 0.2% F. vulgaris ointment and treatment with 0.2% AgNPs@Falcaria ointment. Use of AgNPs@Falcaria ointment in the treatment groups substantially decreased (p ≤ 0.01) the wound area, total cells, neutrophils and lymphocytes and markedly raised (p ≤ 0.01) wound contracture, hydroxyproline, hexosamine, hexuronic acid, fibrocytes and fibrocyte/fibroblast ratio compared to the other groups. In summary, the synthesized AgNPs@Falcaria revealed non‐cytotoxicity and antioxidant, antibacterial, antifungal and cutaneous wound healing activities in a dose‐dependent manner.