Effect of arabinoxylan- and rhamnogalacturonan I-rich polysaccharides isolated from young barley leaf on intestinal immunostimulatory activity

Kim, Hoon; Yu, Kwang-Won; Hong, Hee‐Do; Shin, Kwang‐Soon

doi:10.1016/j.jff.2017.05.052

Cited by 36 publications

(25 citation statements)

References 22 publications

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“…The sugar composition of BLE0 was further analyzed by HPAEC-PAD revealing that BLE0 was mainly composed of xylose, galactose, arabinose, glucose and galacturonic acid, in addition to fucose, rhamnose and glucuronic acid ( Supplementary Material Figure S1 ). In terms of peak area, the high levels of arabinose, xylose, galactose and galacturonic acid indicated that BLE0 possessed arabinoxylan and rhamnogalacturonan I-rich structures, which is in accordance with previous results [ 14 ]. Additionally, the chemical and monosaccharide compositions of BLE0 were similar to those in previous results of BLE0 prepared by a laboratory-scale experiment [ 16 ], indicating stable BLE0 production from the pilot-scale process.…”

Section: Resultssupporting

confidence: 91%

“…Previously, we obtained a polysaccharide fraction (BLE0) from pectinase hydrolysate of young barley leaves with immunostimulatory and antiosteoporotic effects [ 12 , 13 , 14 , 15 , 16 ]. It was demonstrated that pectinase application was a useful method for obtaining polysaccharides with enhanced immune activity from barley leaves [ 12 ].…”

Section: Resultsmentioning

confidence: 99%

“…According to recent research trends, we purified the pectinase-treated polysaccharide fraction (BLE0) from young barley leaves. Furthermore, we reported immune-enhancing and antiosteoporotic activities of BLE0, revealing the detailed structure and molecular mechanisms underlying its immunostimulatory activities [ 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)

Cho

Song

Lim

et al. 2020

Foods

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Polysaccharides isolated from various plants are considered precious bioactive materials owing to their potent biological activities. Previously, we prepared a polysaccharide fraction (BLE0) isolated from young barley leaves (Hordeum vulgare L.), demonstrating its anti-osteoporotic and immunostimulatory activities. However, data regarding BLE0 toxicity is lacking. To establish its safety, in vitro genotoxicity (chromosomal aberration and bacterial reverse mutation assays) and acute oral toxicity assays were conducted. In the in vitro genotoxicity assays, bacterial reverse mutation and chromosomal aberration assays showed that BLE0 possessed no mutagenicity or clastogenicity. Furthermore, the median lethal dose (LD50) of BLE0 was higher than 5000 mg/kg in female and male Sprague-Dawley (SD) rats and no adverse effects were observed in terms of mortality and abnormal changes in clinical signs (body weight and necropsy). Based on these results, BLE0 was found to be safe with regards to genotoxicity under our test conditions, demonstrating no acute oral toxicity up to 5000 mg/kg in SD rats.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)

Cho

Song

Lim

et al. 2020

Foods

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“…It is well established that functional foods may exert their benecial effects on health promotion through cell-to-cell communication networks. 31 In these communication networks, the immune system plays an important role in the body by protecting the organism from infectious disease and tumors. The immune system is mainly composed of immune organs and cells, including the spleen, thymus, lymphocytes and so on.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of a fructooligosaccharide isolated from burdock roots

Zhang

Liu²,

Lü

et al. 2019

RSC Adv.

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“…Yu et al [ 12 ] reported that EPS1-1, extracted from the fermentation broth of Rhizopus nigricans , was an extracellular polysaccharide, which play an important role in the improvement of intestinal function in mice with colorectal cancer. Kim et al [ 13 ] reported that BLE-P, isolated from barley leaves, was a mixture of hemicellulosic glucuronoarabinoxylan and pectic rhamnogalacturonan. The results indicated that BLE-P not only augmented the production of immunoglobulin A (IgA), but also increased the levels of IgA-related cytokines, such as transforming growth factor-b and interleukin-10.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum

et al. 2018

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Polysaccharides from Ascophyllum nodosum (AnPS) were extracted and purified via an optimized protocol. The optimal extraction conditions were as follows: extraction time of 4.3 h, extraction temperature of 84 °C and ratio (v/w, mL/g) of extraction solvent (water) to raw material of 27. The resulting yield was 9.15 ± 0.23% of crude AnPS. Two fractions, named AnP1-1 and AnP2-1 with molecular weights of 165.92 KDa and 370.68 KDa, were separated from the crude AnPS by chromatography in DEAE Sepharose Fast Flow and Sephacryl S-300, respectively. AnP1-1 was composed of mannose, ribose, glucuronic acid, glucose and fucose, and AnP2-1 was composed of mannose, glucuronic acid, galactose and fucose. AnPS, AnP1-1 and AnP2-1 exhibited high scavenging activities against ABTS radical and superoxide radical, and showed protective effect on H2O2-induced oxidative injury in RAW264.7 cells. Furthermore, the immunostimulatory activities of AnP1-1 and AnP2-1 were evaluated by Caco-2 cells, the results showed both AnP1-1 and AnP2-1 could significantly promote the production of immune reactive molecules such as interleukin (IL)-8, IL-1β, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Therefore, the results suggest that AnPS and its two fractions may be explored as a potential functional food supplement.

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Effect of arabinoxylan- and rhamnogalacturonan I-rich polysaccharides isolated from young barley leaf on intestinal immunostimulatory activity

Cited by 36 publications

References 22 publications

Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)

Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)

Immunomodulatory activity of a fructooligosaccharide isolated from burdock roots

Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum

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