Effect of ascorbic acid and curcumin on quercetin-induced nuclear DNA damage, lipid peroxidation and protein degradation

Sahu, Saura C.; Washington, Melissa

doi:10.1016/0304-3835(92)90266-x

Cited by 44 publications

(30 citation statements)

References 24 publications

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“…The potentiating influence of curcumin on the clastogenicity of BLM was reproducible in three independent experiments. A potentiation effect with curcumin was also observed by Sahu and Washington (1992). They demonstrated the pro-oxidant properties of ascorbic acid and curcumin on quercetin-induced nuclear damage in presence of iron and copper.…”

Section: Discussionmentioning

confidence: 70%

“…According to Sahu and Washington (1992), the antioxidant curcumin, like ascorbic acid, can become a pro-oxidant agent depending on the redox state of the biological environment. Therefore, the clastogenic and potentiating effects of curcumin found in the present work could be explained by the fact that curcumin would act as a pro-oxidant agent at the highest concentrations tested under the conditions of the present report.…”

Section: Discussionmentioning

confidence: 99%

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Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Araújo

Dias²,

Kronka

et al. 1999

Genet. Mol. Biol.

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Cell culture and treatmentsChinese hamster ovary cells (CHO-9 cell line) were kindly supplied by Prof. A.T. Natarajan (University of Leiden, The Netherlands). Cells were maintained as monolayers growing at 37°C in 25-cm 2 flasks (Corning) containing HAM-F10 (Sigma) plus DMEM (Dulbecco's modi- ABSTRACT Naturally occurring antioxidants have been extensively studied for their capacity to protect organisms and cells from oxidative damage. Many plant constituents including turmeric and curcumin appear to be potent antimutagens and antioxidants. The effects of turmeric and curcumin on chromosomal aberration frequencies induced by the radiomimetic agent bleomycin (BLM) were investigated in Chinese hamster ovary (CHO) cells. Three concentrations of each drug, turmeric (100, 250 and 500 µg/ ml) and curcumin (2.5, 5 and 10 µg/ml), were combined with BLM (10 µg/ml) in CHO cells treated during the G 1 /S, S or G 2 /S phases of the cell cycle. Neither turmeric nor curcumin prevented BLM-induced chromosomal damage in any phases of the cell cycle. Conversely, a potentiation of the clastogenicity of BLM by curcumin was clearly observed in cells treated during the S and G 2 /S phases. Curcumin was also clastogenic by itself at 10 µg/ml in two protocols used. However, the exact mechanism by which curcumin produced clastogenic and potentiating effects remains unknown.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Araújo

Dias²,

Kronka

et al. 1999

Genet. Mol. Biol.

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“…They also added that phenolic antioxidants are known to stop lipid peroxidation of cell membranes, a prominent free radical chain reaction among unsaturated fatty acids that is carcinogenic by virtue of being both mutagenic and mitogenic. Some of these activities are responsible for the ability of curcumin to protect DNA against free radical-induced damage and to protect biological cells against various toxins (Sahu and Washington 1992).…”

Section: Discussionmentioning

confidence: 99%

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Fattah

He²,

Fa³

et al. 2010

gpb

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Abstract. This study aimed to investigate the possible protective role of curcumin against renal damage caused by administration of cyclosporine A (CsA) in adult male rats. For this purpose, 27 adult male albino rats were used and divided into three equal groups. Group I (control group) and group II (CsA-treated group) received a daily subcutaneous injection of CsA at a dose of 20 mg/kg b.w. Group III (prophylactic group) received a daily oral curcumin at a dose of 15 mg/kg b.w. simultaneously with CsA. After 21 days, all the animals were anaesthetized and the kidneys were rapidly removed and processed to prepare paraffin sections stained with H&E, PAS and Masson's trichrome. In addition, the glutathione S-transferase (GST) enzyme was detected immunohistochemically. The optical density and the area (in %) of positive GST immunoreactions were measured in the cytoplasm of renal tubules and glomeruli and the data were statistically analyzed. Examination of sections from CsA-treated group showed renal tubules with vacuolated cytoplasm and others with darkly stained pyknotic nuclei. Apical brush borders of proximal tubules were undefined and PAS positive granules were noticed in their cytoplasm. The renal corpuscles contained shrunken glomeruli with widening of their Bowman's spaces. Inflammatory cellular infiltrate and increase in the collagen fibers were observed between the renal tubules. In prophylactic group, the structure of renal tubules and corpuscles were preserved except few tubular darkly stained pyknotic nuclei. Numerous blood vessels, few cellular infiltration and thin collagen fibers were observed between the renal tubules. Statistical analysis of morphometric data showed significant increase in the optical density of GST immunoreactivity in the cells of renal tubules and glomeruli of CsAtreated group when compared with the control or prophylactic groups. However, a significant decrease in the area of GST immunoreactivity in sections from prophylactic group was observed when compared with control or CsA-treated groups. In conclusion, protective effect of curcumin against cyclosporine-induced nephrotoxicity in rats was proven based on the study of histological changes and GST immunoexpression. This study supposes the possible therapeutic applications of curcumin in CsA-treated patients.

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“…S-vanillin were enhanced the chromosome aberrations induced by alkylating agents in cultured Chinese hamster cells [25]. Bcarotene and ascorbic acid were enhanced the clastogenicity induced by BLM in CHO cells [26and27].Curcumin, like ascorbic acid, can become a pro-oxidant agent depending on the redox state of the biological environment [28]. Therefore, the mutagenic effects of curcumin found in the present work could be explained by the fact that curcumin would act as a pro-oxidant agent at the highest concentrations tested under the conditions of the present study.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetical and histochemical studies on curcumin in male rats

Makawy¹,

Sharaf²

2006

WIT Transactions on Biomedicine and Health, Vol 10

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Curcumin is a major component of the curcuma species, commonly used as a yellow coloring and flavoring agent in foods. Curcumin has been demonstrated to have potent antioxidant, anti-inflammatory activity and has shown anticancer properties in many rodent models. There is the perception that since compounds are natural, they are devoid of toxicity and safe to use. Some of the active compounds in supplements have inherent toxicity. In this study, five doses (0.5, 5, 10, 25 and 50 mg/kg/daily) of curcumin spice were orally administered to male rats daily for four weeks. The effect of curcumin was studied genetically by evaluation of chromosomal aberrations and micronucleus formation in bone marrow cells of male rats. Histopathological and histochemical investigations were studied in different tissues (liver, kidney) of males. The cytogenetical results showed that curcumin caused a statistically significant dose-dependent increase in the number of micronucleated polychromatic erythrocytes (MNPCEs) and in the frequencies of total chromosomal aberrations over the control. Also, results showed that there were significant differences between positive control and all curcumin-tested doses, except the dose of 50 mg/kg showed no significant difference. Histopathological results showed different degrees of alterations, as manifested by vacuolar degeneration in hepatocytes, tubular degeneration in renal tissues. We conclude that over use of curcumin spice may cause genotoxical and histopathological effects.

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Effect of ascorbic acid and curcumin on quercetin-induced nuclear DNA damage, lipid peroxidation and protein degradation

Cited by 44 publications

References 24 publications

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Cytogenetical and histochemical studies on curcumin in male rats

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