Effect of ASCs on the graft survival rates of fat particles in rabbits

He, Xiaosheng; Zhong, Xiaochun; Ni, You-Di; Liu, Maolin; Liu, Suhang; Lan, Xiaoling

doi:10.3109/2000656x.2012.730488

Cited by 30 publications

(12 citation statements)

References 20 publications

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“…In our past experiments, we observed that excellent results were obtained when fat particles mixed with SVF were grafted. 25 Obviously, similar effects were demonstrated in this experiment. Therefore, we propose that there may be beneficial effects between SVF and fat tissue when transplanting the mixture of both.…”

Section: Graft Survival Ratessupporting

confidence: 87%

Effect of Fat Particle-to-SVF Ratio on Graft Survival Rates in Rabbits

Ni¹,

He²,

Yuan³

et al. 2015

Annals of Plastic Surgery

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Section: Graft Survival Ratessupporting

confidence: 87%

Effect of Fat Particle-to-SVF Ratio on Graft Survival Rates in Rabbits

Ni¹,

He²,

Yuan³

et al. 2015

Annals of Plastic Surgery

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“…Five studies were identified, in which immunocompetent animals were used of which two used rodents (allogeneic cells) [ 16 , 50 ] and the remaining studies used rabbits (autologous cells) [ 17 , 51 , 52 ]. The relative survival improvement for CAL ranged from 1.29- to 4.14-fold compared with their respective control group.…”

Section: Resultsmentioning

confidence: 99%

Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy

2016

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Introduction Autologous lipotransfer is seen as an ideal filler for soft tissue reconstruction. The main limitation of this procedure is the unpredictable resorption and volume loss of the fat graft. In the recent decade, an increasing amount of research has focused on the use of adipose tissue-derived stromal cells (ASCs) to enrich the fat graft, a procedure termed cell-assisted lipotransfer (CAL). The aim of this review was to systematically review the current preclinical and clinical evidence for the efficacy of CAL compared with conventional lipotransfer.Materials and MethodsA systematic search was performed on PubMed and other databases to identify all preclinical and clinical studies where CAL with ASCs was compared with conventional lipotransfer. A total of 20 preclinical studies and seven clinical studies were included in the review.ResultsThe preclinical studies consisted of 15 studies using immunodeficient animal models and five studies using immunocompetent studies. Seventeen studies examined weight/volume retention of which 15 studies favored CAL over conventional lipotransfer. One clinical study did not find any efficacy of CAL and the remaining six studies favored CAL.ConclusionsThe present evidence suggests that there is a big potential for CAL in reconstructive surgery; however, the present studies are so far still of low quality with inherent weaknesses. Several aspects regarding CAL still remain unknown such as the optimal degree of cell enrichment and also its safety. Further high-quality studies are needed to establish if CAL can live up to its potential.Level of Evidence VThis journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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“…The positive effects of ASC-based approaches in regenerative therapies have been demonstrated in preclinical and clinical studies, for example in cell-assisted lipotransfer or treatment of ischemic diseases [ 9 , 28 , 29 , 30 ]. However, a substantial loss of implanted cells has been documented during the early phase of engraftment [ 12 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells

Bachmann

Ehlert

Becker

et al. 2020

Cells

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Adipose-derived stromal/stem cells (ASCs) have been shown to exert regenerative functions, which are mainly attributed to the secretion of trophic factors. Upon transplantation, ASCs are facing an ischemic environment characterized by oxygen and nutrient deprivation. However, current knowledge on the secretion capacity of ASCs under such conditions is limited. Thus, the present study focused on the secretory function of ASCs under glucose and oxygen deprivation as major components of ischemia. After exposure to glucose/oxygen deprivation, ASCs maintained distinct viability, but the metabolic activity was greatly reduced by glucose limitation. ASCs were able to secrete a broad panel of factors under glucose/oxygen deprivation as revealed by a cytokine antibody array. Quantification of selected factors by ELISA demonstrated that glucose deprivation in combination with hypoxia led to markedly higher secretion levels of the angiogenic and anti-apoptotic factors IL-6, VEGF, and stanniocalcin-1 as compared to the hypoxic condition alone. A conditioned medium of glucose/oxygen-deprived ASCs promoted the viability and tube formation of endothelial cells, and the proliferation and migration of fibroblasts. These findings indicate that ASCs are stimulated by ischemia-like stress conditions to secrete trophic factors and would be able to exert their beneficial function in an ischemic environment.

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Effect of ASCs on the graft survival rates of fat particles in rabbits

Cited by 30 publications

References 20 publications

Effect of Fat Particle-to-SVF Ratio on Graft Survival Rates in Rabbits

Effect of Fat Particle-to-SVF Ratio on Graft Survival Rates in Rabbits

Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy

Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells

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