Effect of Fat Particle-to-SVF Ratio on Graft Survival Rates in Rabbits

Ni, You-Di; He, Xiaosheng; Yuan, Zhaohui; Liu, Maolin; Du, Hongying; Zhong, Xiaochun

doi:10.1097/sap.0b013e318298e6f5

Cited by 18 publications

(12 citation statements)

References 25 publications

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“…Five studies were identified, in which immunocompetent animals were used of which two used rodents (allogeneic cells) [ 16 , 50 ] and the remaining studies used rabbits (autologous cells) [ 17 , 51 , 52 ]. The relative survival improvement for CAL ranged from 1.29- to 4.14-fold compared with their respective control group.…”

Section: Resultsmentioning

confidence: 99%

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Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy

2016

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Introduction Autologous lipotransfer is seen as an ideal filler for soft tissue reconstruction. The main limitation of this procedure is the unpredictable resorption and volume loss of the fat graft. In the recent decade, an increasing amount of research has focused on the use of adipose tissue-derived stromal cells (ASCs) to enrich the fat graft, a procedure termed cell-assisted lipotransfer (CAL). The aim of this review was to systematically review the current preclinical and clinical evidence for the efficacy of CAL compared with conventional lipotransfer.Materials and MethodsA systematic search was performed on PubMed and other databases to identify all preclinical and clinical studies where CAL with ASCs was compared with conventional lipotransfer. A total of 20 preclinical studies and seven clinical studies were included in the review.ResultsThe preclinical studies consisted of 15 studies using immunodeficient animal models and five studies using immunocompetent studies. Seventeen studies examined weight/volume retention of which 15 studies favored CAL over conventional lipotransfer. One clinical study did not find any efficacy of CAL and the remaining six studies favored CAL.ConclusionsThe present evidence suggests that there is a big potential for CAL in reconstructive surgery; however, the present studies are so far still of low quality with inherent weaknesses. Several aspects regarding CAL still remain unknown such as the optimal degree of cell enrichment and also its safety. Further high-quality studies are needed to establish if CAL can live up to its potential.Level of Evidence VThis journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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Section: Resultsmentioning

confidence: 99%

“…Ni et al performed a study that compared four different fat tissue to SVF ratios in an immunocompetent rabbit model [ 52 ]. They used a total of 0.8 g with varying ratios between fat and SVF.…”

Section: Resultsmentioning

confidence: 99%

Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy

2016

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“…The search of publications completed for the current study yielded 15 murine ( Table I ) ( 48 , 52 , 57 – 69 ) and 19 human ( Table II ) ( 30 , 53 – 55 , 70 – 84 ) studies that assessed the effect of fat graft enrichment with freshly isolated ADSCs (SVF) or ex vivo cultured ADSCs. A total of 24 of the studies compared SVF assisted lipotransfer to traditional lipotransfer (control), 10 compared ex vivo expanded ADSC-enriched fat grafting to controls and one animal study compared both.…”

Section: Experimental and Clinical Trials With Adsc-assisted Fat Grafmentioning

confidence: 99%

“…Part of the lipoaspirate was used to extract the SVF, which was either used directly as a source of ADSCs or used for ex vivo expansion of the ADSCs. The majority of animal studies (n=11) investigated immunosuppressed mice (severe combined immunodeficiency (SCID) or nude mice) and only three investigated rabbits ( 59 , 60 , 69 ). Nude mice are the most commonly used murine model for xenografting.…”

Section: Experimental and Clinical Trials With Adsc-assisted Fat Grafmentioning

confidence: 99%

“…Ni et al ( 69 ) performed a study that compared different mixtures of fat particles with various proportions of SVF and identified that 3:1 is the optimal fat particle-to-SVF ratio, exhibiting the greatest weight maintenance. The two latter studies suggest that there may be a minimum requirement for the amount of cells administered in order to achieve a beneficial effect.…”

Section: Optimal Cell Populationmentioning

confidence: 99%

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Application of adipose-derived stromal cells in fat grafting: Basic science and literature review

Moustaki

Papadopoulos

Verikokos

et al. 2017

Experimental and Therapeutic Medicine

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Autologous fat is considered the ideal material for soft-tissue augmentation in plastic and reconstructive surgery. The primary drawback of autologous fat grafting is the high resorption rate. The isolation of mesenchymal stem cells from adipose tissue inevitably led to research focusing on the study of combined transplantation of autologous fat and adipose derived stem cells (ADSCs) and introduced the theory of ‘cell-assisted lipotransfer’. Transplantation of ADSCs is a promising strategy, due to the high proliferative capacity of stem cells, their potential to induce paracrine signalling and ability to differentiate into adipocytes and vascular cells. The current study examined the literature for clinical and experimental studies on cell-assisted lipotransfer to assess the efficacy of this novel technique when compared with traditional fat grafting. A total of 30 studies were included in the present review. The current study demonstrates that cell-assisted lipotransfer has improved efficacy compared with conventional fat grafting. Despite relatively positive outcomes, further investigation is required to establish a consensus in cell-assisted lipotransfer.

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