Effect of ATP-binding cassette subfamily B member 1 on bovine blastocyst implantation

Mori, Masayuki; Kuwano, T.; Kamori, Tsugumitsu; Isozaki, Y.; Nishihara, Takuji; Yamauchi, Nobuhiko; Hattori, Masa Aki

doi:10.1016/j.theriogenology.2013.11.015

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…The GO term of calcium ion binding could be associated with hypocalcemia in cattle (Pacheco et al, 2018). Previous studies showed that the GO term of ATP binding was associated with drug transport and milk fat metabolism in bovine mammary tissue, as well as with reproduction in cattle (Chen et al, 2019;Mori et al, 2014;Otero et al, 2013Otero et al, , 2016.…”

Section: Differential Cnv Segments Selected In Swamp Buffaloes and Ri...mentioning

confidence: 99%

Comparative analyses of copy number variations between swamp buffaloes and river buffaloes

Han

Deng

et al. 2023

Animal Genetics

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As an important source of genomic variation, copy number variation (CNV) contributes to environmental adaptation in worldwide buffaloes. Despite this importance, CNV divergence between swamp buffaloes and river buffaloes has not been studied previously. Here, we report 21 152 CNV regions (CNVRs) in 141 buffaloes of 20 breeds detected through multiple CNV calling strategies. Only 248 CNVRs were shared between river buffalo and swamp buffalo, reflecting great variation of CNVRs between the two subspecies. Population structure analysis based on CNVs successfully separated the two buffalo subspecies. We further assessed CNV divergence by calculating FST for genome‐wide CNVs. Totally, we identified 110 significantly divergent CNV segments and 44 putatively selected genes between river buffaloes and swamp buffaloes. In particular, LALBA, a key gene controlling milk production in cattle, presented a highly differentiated CNV in the promoter region, which makes it a strong functional candidate gene for differences between swamp buffaloes and river buffaloes in traits related to milk production. Our study provides useful information of CNVs in buffaloes, which may help explain the genetic differences between the two subspecies.

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Section: Differential Cnv Segments Selected In Swamp Buffaloes and Ri...mentioning

confidence: 99%

Comparative analyses of copy number variations between swamp buffaloes and river buffaloes

Han

Deng

et al. 2023

Animal Genetics

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“…Modification of embryo cryopreservation could improve the pregnancy rate by employing in vitro frozen ET. It is likely that culturing blastocysts with some cryoprotectants before freezing or using vitrification could increase the cryotolerance, improve embryo survival after thawing [ 89 , 90 , 91 ], and increase the ET pregnancy rate in summer [ 87 ]. Moreover, the genetic differences affecting thermotolerance in oocytes or embryos have been reported between breeds of cattle [ 38 , 80 , 92 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Effects of heat stress on bovine preimplantation embryos produced <i>in vitro</i>

Sakatani

2017

Journal of Reproduction and Development

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Summer heat stress decreases the pregnancy rate in cattle and has been thought to be associated with the early embryonic death caused by the elevation of maternal body temperature. In vitro cultures have been widely used for the evaluation of effects of heat stress on oocytes, fertilization, preimplantation, and embryonic development. Susceptibility to heat stress is present in developmental stages from oocytes to cleavage-stage (before embryonic gene activation, EGA) embryos, leading to a consequent decrease in developmental competence. On the other hand, advanced-stage embryos such as morula or blastocysts have acquired thermotolerance. The mechanism for the developmental stage-dependent change in thermotolerance is considered to be the accumulation of antioxidants in embryos in response to heat-inducible production of reactive oxygen species. The supplementation of antioxidants to the culture media has been known to neutralize the detrimental effects of heat stress. Besides, EGA could be involved in acquisition of thermotolerance in later stages of embryos. Morulae or blastocysts can repair heat-induced unfolded proteins or prevent DNA damage occurring in processes such as apoptosis. Therefore, embryo transfer (ET) that can bypass the heat-sensitive stage could be a good solution to improve the pregnancy rate under heat stress. However, frozen-thawed ET could not improve the pregnancy rate as expected. Frozen-thawed blastocysts were more sensitive to heat stress and showed less proliferation upon heat exposure, compared to fresh blastocysts. Therefore, further research is required to improve the reduction in pregnancy rates due to summer heat stress.

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“…All cultures were maintained at 38.5 C in a humidified atmosphere of 3% CO 2 , 10% O 2 and 87% N 2 . Frozen semen from a Japanese black bull was used for in vitro fertilization, as previously described [ 30 ]. The embryos were then cultured with M199 as described in experiment 1.…”

Section: Methodsmentioning

confidence: 99%

“…Cryopreservation was performed as described in a previous report [ 30 ]. Briefly, blastocysts obtained at day 7 were transferred to a cryoprotective solution (5% [v/v] ethylene glycol, 6% [v/v] propylene glycol, 0.1 M sucrose and 4 mg/ ml bovine serum albumin [BSA] in Dulbecco’s phosphate-buffered saline [D-PBS]), and then 1 or 2 blastocysts were introduced into a 0.25-ml straw (IMV Technologies, L’Aigle, France) at 23–25 C for 13 min of equilibration.…”

Section: Methodsmentioning

confidence: 99%

Heat shock decreases the embryonic quality of frozen-thawed bovine blastocysts produced <i>in vitro</i>

Mori

Hayashi

Isozaki

et al. 2015

Journal of Reproduction and Development

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In this study, the effect of heat shock on frozen-thawed blastocysts was evaluated using in vitro-produced (IVP) bovine embryos. In experiment 1, the effects of 6 h of heat shock at 41.0 C on fresh blastocysts were evaluated. HSPA1A expression as a reflection of stress was increased by heat shock (P < 0.05), but the expressions of the quality markers IFNT and POU5F1 were not affected. In experiment 2, frozen-thawed blastocysts were incubated at 38.5 C for 6 h (cryo-con) or exposed to heat shock at 41.0 C for 6 h (cryo-HS). Then, blastocysts were cultured at 38.5 C until 48 h after thawing (both conditions). Cryo-HS blastocysts exhibited a decreased recovery rate: HSPA1A expression was dramatically increased compared with that in fresh or cryo-con blastocysts at 6 h, and IFNT expression was decreased compared with that in cryo-con blastocysts at 6 h (both P < 0.05). Cryo-con blastocysts at 6 h also exhibited higher HSPA1A expression than fresh blastocysts (P < 0.05). At 48 h after thawing, the number of hatched blastocysts and blastocyst diameter were lower in cryo-HS blastocysts (P < 0.05). Cryo-con blastocysts showed lower POU5F1 levels at 48 h than fresh, cryo-con or cryo-HS blastocysts at 6 h (P < 0.05), but their POU5F1 levels were not different from those of cryo-HS blastocysts at 48 h. These results indicated that application of heat shock to frozen-thawed blastocysts was highly damaging. The increase in damage by the interaction of freezing-thawing and heat shock might be one reason for the low conception rate in frozen-thawed embryo transfer in summer.

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Effect of ATP-binding cassette subfamily B member 1 on bovine blastocyst implantation

Cited by 4 publications

References 22 publications

Comparative analyses of copy number variations between swamp buffaloes and river buffaloes

Comparative analyses of copy number variations between swamp buffaloes and river buffaloes

Effects of heat stress on bovine preimplantation embryos produced <i>in vitro</i>

Heat shock decreases the embryonic quality of frozen-thawed bovine blastocysts produced <i>in vitro</i>

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