Effect of Autologous Adipose-Derived Stem Cells in Renal Cold Ischemia and Reperfusion Injury

Melatonin pretreatment of human adipose tissue-derived mesenchymal stromal cells enhances their prosurvival and protective effects on human kidney cells

“…In previous in vivo studies, we and others used nontreated human marrowderived MSCs and showed the effectiveness of these cells when delivered in immunodeficient mice with cisplatin-induced AKI to decrease mouse mortality and protect renal function (11,27). Other reports have shown the therapeutic potential of ASCs in kidney injury models (8,14,43). In brief, mouse and rat ASCs provided renoprotective effects when administered in rodents subjected to kidney ischemia-reperfusion injury (8,14,43).…”

“…Other reports have shown the therapeutic potential of ASCs in kidney injury models (8,14,43). In brief, mouse and rat ASCs provided renoprotective effects when administered in rodents subjected to kidney ischemia-reperfusion injury (8,14,43). Observations included antiapoptotic, antioxidative, and anti-inflammatory effects (8,14,43).…”

“…In brief, mouse and rat ASCs provided renoprotective effects when administered in rodents subjected to kidney ischemia-reperfusion injury (8,14,43). Observations included antiapoptotic, antioxidative, and anti-inflammatory effects (8,14,43). Furthermore, in a model of cisplatin-induced AKI, mouse ASCs, as well as their CM, showed protective effects by decreasing kidney injury and tubular cell apoptosis, and increasing mouse survival (2).…”

Immunomodulatory effects of OX40Ig gene-modified adipose tissue-derived mesenchymal stem cells on rat kidney transplantation

“…ADSCs were isolated from LEW rats (n=72) using a previously described method (17). Briefly, the rats were anesthetized with isoflurane inhalation (LUNAN Pharmaceutical Co., Ltd., Shandong, China) 14 days before mixed lymphocyte reaction (MLR) assay and autologous cell transplantation, and the subcutaneous adipose tissue was then obtained from the hemi-inguinal regions of these rats, which were carefully excised and minced into ~1 mm 3 sections.…”

“…DSCs were also confirmed by their capacity to differentiate into adipogenic, islet and osteogenic lineages as previously described (17). Briefly, the ADSCs were seeded in medium at 2×10 4 cells/cm 2 in 6-well tissue culture plates.…”