Effect of auxin, cytokinin and nitrogen on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f.niedzwetzkyana)

Ji, Xiaohao; Wang, Yanting; Zhang, Rui; Wu, Shu-Jing; An, Malaviya; Min, Li; Wang, Chuan‐Zeng; Chen, Xiaoliu; Zhang, Yanmin; Chen, Xuesen

doi:10.1007/s11240-014-0609-y

Cited by 114 publications

(81 citation statements)

References 53 publications

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“…1D). Cytokinins have been shown to enhance anthocyanin accumulation in in vitro cultures of Ajuga reptans (Callebaut et al 1997), Arabidopsis thaliana (Deikman and Hammer 1995), and Malus sieversii f. niedzwetzkyana (Ji et al 2015). The regenerated shoots rooted (100%) with a mean number of 9.4 roots per shoot on auxins-free MS medium.…”

Section: Resultsmentioning

confidence: 99%

Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge

Kim

Sivanessan

2016

Braz. arch. biol. technol.

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Effects of N 6 -benzyladenine (BA) or thidiazuron (TDZ) on adventitious shoot regeneration and axillary shoot multiplication of Sedum sarmentosum was investigated. Leaf and shoot tip explants obtained from in vitro-grown shoots of S. sarmentosum were cultured on Murashige and Skoog (MS) medium supplemented with 0, 2.0, 4.0 or 8.0 µM BA or TDZ. Of the two cytokinins studied, BA was found to be more responsive as compared to TDZ with respect to number of shoots produced per explant. High frequency of shoot regeneration (92.2%) with a mean of 12.3 shoots was produced when the leaf explants were cultured on MS medium supplemented with 8.0 µM BA. The highest number of shoots (25.4) was obtained when shoot tip explants were cultured on MS medium devoid of cytokinins after 35 days of culture. For root induction, regenerated shoots were cultured on MS medium supplemented with 0, 2.0, 4.0 or 8.0 µM indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). The highest number of (17.6) roots per shoot was obtained on MS medium supplemented with 2.0 µM IBA after 30 days of culture. Regenerated plantlets were successfully acclimatized in the greenhouse with 100% survival rate.

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Section: Resultsmentioning

confidence: 99%

Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge

Kim

Sivanessan

2016

Braz. arch. biol. technol.

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“…Reportedly, root or shoot explants of Arabidopsis culture in callus-inducing medium (CIM) added with cytokinin and auxin induced callus from pericycle cells adjacent to the xylem poles. Additionally, shoot and root regeneration seemed to partly resemble the lateral root meristem formation (Jia et al 2014;Ji et al 2015). Interestingly, the root or shoot calli are not unorganized cells mass, as they were organized structures similar to the primordia of lateral roots (Jia et al 2014).…”

Section: Effect Of Various Explants On Callus Frequencymentioning

confidence: 99%

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, L-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L -1 of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg

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“…1a). The red callus culturing method was based on a published procedure (Ji et al 2015). The callus induction medium consisted of Murashige and Skoog medium supplemented with 0.6 mg l -1 2,4-dichlorophenoxyacetic acid, 0.5 mg l -1 thidiazuron, 30 g l -1 sugar, and 7 g l -1 agar.…”

Section: Plant Materials and Callus Inductionmentioning

confidence: 99%

“…These findings are consistent with the results of previous studies, indicating that temperature and light regulate anthocyanin biosynthesis not only in red-skinned cultivated apples, but also in red-fleshed apples and red callus tissue. Thus, anthocyanin metabolism may be relatively stable in response to different temperatures and light Ji et al (2015) studied anthocyanin accumulation in the same red callus at different concentration of auxins and/or cytokinins, and suggested that anthocyanin accumulation decreased with increased auxin (NAA and 2,4-D) concentration, while nitrogen deficiency could reverse the inhibition of anthocyanin synthesis by auxins. Both growth regulators and nitrogen affected the primary metabolism of the plant.…”

Section: Expression Levels Of Anthocyanin Pathway Genes and Related Tmentioning

confidence: 99%

“…Wang et al (2010) studied a red-fleshed apple individual ('Zihong 1') obtained from segregating populations, and determined that its flesh contains extremely high anthocyanin contents. Ji et al (2015) induced callus formation using a red-fleshed apple strain, and observed that increasing auxin concentrations can significantly inhibit anthocyanin biosynthesis. In this study, we generated a red callus using the leaves of 'Zihong 1' apple trees.…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effects of light and temperature on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)

Wang

Zhang

Jiang

et al. 2016

Plant Cell Tiss Organ Cult

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We established a red callus from the leaves of a red-fleshed apple individual, which was a hybrid offspring of the cross between Malus sieversii f. niedzwetzkyana and Malus domestica cv. 'Fuji'. We analyzed callus growth and anthocyanin biosynthesis/metabolism under different combinations of temperature and light conditions. Incubation in darkness resulted in decreased anthocyanin accumulation, while it promoted callus growth. Exposure to light and low temperature (16°C) induced the expression of MYB10 and bHLH3/33, which are responsible for coordinating the regulation of anthocyanin biosynthesis, as well as the expression of other structural genes. Treatments with light and high temperature (32°C) induced MYB16 expression, which repressed anthocyanin biosynthesis. Additionally, low temperature (16°C) inhibited the expression of MYB111. We analyzed the expression patterns of MYB and bHLH transcription factor genes by quantitative real-time polymerase chain reaction. Our data suggest that light-induced regulation of anthocyanin biosynthesis is primarily caused by altered MYB10 transcript levels, while temperature-induced regulation is the result of changes to the expression of bHLH3/33, MYB16, MYB17, MYB111, and other repressors. In conclusion, we investigated the reciprocal effects of light and temperature on anthocyanin biosynthesis in red-fleshed apple calli. Our findings may provide a theoretical basis for breeding redfleshed apple varieties with high anthocyanin contents.

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Effect of auxin, cytokinin and nitrogen on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f.niedzwetzkyana)

Cited by 114 publications

References 53 publications

Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge

Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Synergistic effects of light and temperature on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)

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