Effect of Azadirachta indica leaf methanol extracts on stem cell reproduction

González‐Garza, María Teresa; Codinach, Margarita; Alcaraz, Citlali; Moreno-Cuevas, Jorge E.; Carranza-Rosales, Pilar; Cruz-Vega, Delia Elva

doi:10.1016/j.fitote.2006.11.016

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…The elucidation of the neem genome was a gradual process, culminating in its completion in 2011 [7] . Rapid advancements in sequencing technology have propelled comprehensive analysis of the neem genome and Leaves, fruits, flowers Liquid-solid extraction -Acetone, Ethanol, Methanol UHPLC Q-Orbitrap [91] Seed, Leaf Ultrasonic-assisted method 40 min acetonitrile LC-Q-TOF-MS [76] Seed Liquid-solid extraction 48 h n-Hexane HPLC-UV [92] Seed Schroeder and Nakanishi Method 30 min Alcohol SFC [93] Kernels Soxhlet extraction 2 h n-Hexane HPLC [79] Leaves, bark, roots Soxhlet extraction -Water LC-MS [94] Fruit Reflux extraction 48 h Alcohol HPLC-UV [92] Seed Thorns extraction 24-72 h Carbinol HPLC-UV [72] Seed Screw press 20 min -- [95] Leaf Pressurized liquid extraction -Carbinol HPLC-UV [71] Bark Shaking -Methanol Spectrophotometric [96] Seed Supercritical fluid extraction 150 min Methanol HPLC [97] Kernels Cold extraction -Ethanol HPLC [98] Leaf Cold extraction 48 h Petroleum ether - [99] Seeds Cold-pressing -Hexane HPLC-UV [100] Fruits Ultrasound-assisted extraction 30 min Ethanol HPLC-UV [82] Seeds Maceration 3 d Methanol HPLC-UV [71] Leaf Percolation, decoction, freeze drying or spray drying 6 h Water TLC [101] Fruits Percolation -Hexane HPLC-UV [102] HPLC: High performance liquid chromatography; LC-MS: Liquid chromotography with mass spectrometry; LC-Q-TOF-MS: Ultra-high performance liquid chromatography coupled with quadrupole/ time of flight mass spectrometry; SFC: Supercritical fluid chromatography; TLC: Thin layer chromatography; UHPLC: Ultra high performance liquid chromatography; UPLC-QTOF: Ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry.…”

Section: Progress In Transcriptome/genome Researchmentioning

confidence: 99%

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

Su,

Liang,

Xue

et al. 2023

Acupuncture and Herbal Medicine

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Azadirachtin, a complex tetratriterpenoid limonin with potent insecticidal properties, is the most widely used biological pesticide worldwide. Its versatile pharmacological applications include the inhibition of tumor growth and anti-malarial, anti-bacterial, and anti-inflammatory properties. Azadirachtin plays a pivotal role in pest control and novel drug development. The primary source of azadirachtin is the neem tree (Azadirachta indica A. Juss), with an azadirachtin content ranging from 0.3% to 0.5%. Despite the market demand for botanical pesticides reaching approximately 100,000 tons per year, the annual neem production in China is only 1.14 tons. Although azadirachtin can be obtained through plant extraction or chemical synthesis, the quantity obtained does not meet the market demand in China. The sluggish pace of azadirachtin biosynthesis results from the limited availability of genetic information and the complexity of the synthetic pathway. Recent advancements in azadirachtin biosynthesis hold promise as an efficient collection method. In this study, we explored the physicochemical properties, biological activities, mechanisms of action, and acquisition methods of azadirachtin. We also delved into recent progress in azadirachtin biosynthesis and assessed potential future usage challenges. This study aims to establish a theoretical foundation for the scientific application and efficient synthesis of azadirachtin, offering valuable reference information to the industry.

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Section: Progress In Transcriptome/genome Researchmentioning

confidence: 99%

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

Su,

Liang,

Xue

et al. 2023

Acupuncture and Herbal Medicine

View full text Add to dashboard Cite

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Leucocytic profile of rats experimentally infected with Trypanosoma brucei brucei and treated with a combination of methanolic leaf extracts of Azadirachta indica and diminazene diaceturate

et al. 2011

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Chemistry, bioactivities, extraction and analysis of azadirachtin: State-of-the-art

et al. 2019

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Azadirachta indica A. Juss. (Neem) is an Indian tree recognized for its activity as pesticide, as well as several pharmacological properties. Among the various compounds already isolated and studied from Neem tree, azadirachtin (AZA) was identified as the main bioactive compound. Azadirachtin can be found at different parts of the Neem plant but assumes its maximum concentration at the seed level. This compound features a quite complex chemical structure, which justifies the 20-plus-year difficulty to identify the synthetic pathway that subsequently permitted to carry out its artificial synthesis. Azadirachtin is widely used as a basis for production of biopesticides; nevertheless, other properties have been recognized for this substance, among which the an-ticancer and antimalarial activity stand out. The methods available for azadirachtin extraction are diverse, including solid-liquid extraction and extraction with solvents at high or low temperatures. Alcohol based solvents are associated with higher extraction yields and are therefore preferred for the isolation of azadirachtin from plant parts. Clean-up of the extracts is generally required for further purification. The highest azadirachtin levels have been obtained from Neem seeds but concentration values present a large variation between batches. Therefore, in addition to extraction procedures, it is essential to establish routine methods for azadirachtin identification and quantification. Chromatography-based techniques are preferably selected for detection and quantification of azadirachtin in plant matrices. Overall, this process will guarantee a future reproducible, safe and effective use of the extracts in formulations for commercial applications.

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Effect of Azadirachta indica leaf methanol extracts on stem cell reproduction

Cited by 4 publications

References 9 publications

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

Leucocytic profile of rats experimentally infected with Trypanosoma brucei brucei and treated with a combination of methanolic leaf extracts of Azadirachta indica and diminazene diaceturate

Chemistry, bioactivities, extraction and analysis of azadirachtin: State-of-the-art

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