Effect of BA Treatments on Morphology and Physiology of Proliferated Shoots of <i>Bambusa vulgaris</i> Schrad. Ex Wendl in Temporary Immersion

Abstract

Axillary buds, collected from greenhouse-grown plants of Bambusa vulgaris Schrad. ex Wendl (B. vulgaris), were incubated on a static liquid culture medium, Murashige and Skoog (MS) medium with 2% (w/v) sucrose, and supplemented with 12.0 µM 6-benzyladenine (BA). They were transferred to a temporary immersion system (TIS) using liquid MS medium supplemented with 0 (CK-free medium), 6.0, 12.0, 18.0 µM BA. The morphological and anatomical indicators were measured. The BA influenced in vitro multiplication of B. vulgaris. The best results were achieved in the SIT with a concentration of 6.0 µM of BA, which increased the number of shoots (5.1 shoots/explant) in the absence of hyperhydric shoots. Results demonstrated that the water content in the sprouts increased with 12.0 and 18.0 µM BA every four hours. Furthermore, these high levels of BA contributed to a lower accumulation of phenolic compounds and lignin content. The total chlorophyll significantly increased when using 6.0 uM BA, but decreased both parameters with other treatments. These results favor to increase the number of shoots/explants during in vitro multiplication. They will also optimize the in vitro culture conditions, leading to an improvement of in vitro propagation methods for this species.

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

“…Within each growth conditions (ST or PAM), we observed higher phenol content and antioxidant activity with increased sucrose in the culture medium. The addition of sucrose to V. condensata leads to an increase in phenolic and flavonoid compounds [70], and in bamboo [82], rose [83], teak [42] and olive [84], the phenol content was associated with treatments producing more and longer shoots.…”

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA

“…In the case of banana, increases have been achieved in high average multiplication rates, with improved plant quality (Albany et al, 2005;Berthouly and Etienne, 2005;Watt, 2012;Pérez et al, 2013). Only a few bamboo species such as Dendrocalamus latiflorus (Mongkolsook et al, 2005 cited by García-Ramirez et al, 2014), Bambusa ventricosa and Dracaena deremensis (Chaille, 2011) and Bambusa vulgaris (García-Ramírez et al, 2014) have been propagated by TIS. This study aimed to optimize shoot production for micropropagation of G. angustifolia for commercial purposes.…”