Effect of Bacteriocin of Paenibacillus Polymyxa on Biofilm Forming Listeria Monocytogenes MTCC 657

Krishna, Arya Radhakrishnan

doi:10.21786/bbrc/13.2/66

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“…Similar results were observed in 48h and 72h. From the results, it is clear that Listeria monocytogenes MTCC 657 has higher ability to form biofilm on these abiotic substrates Krishna et al 30 The absorbance values of biofilm adherence of Listeria monocytogenes MTCC 657, stainless steel maintained higher biofilm formation than aluminum foil and glass (Fig. 3.).…”

Section: Tube Methodsmentioning

confidence: 85%

“…The biofilm production seems to be increasing as the incubation increases. 30 Biofilm formation is a complicated process which is influenced by factors such as time, temperature and growth medium. 42 Biofilm formation on different substrates Three abiotic surfaces glass, aluminum foil and stainless steel were selected for different incubation periods.…”

Section: Tube Methodsmentioning

confidence: 99%

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Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657

Krishna¹,

Jayalekshmi²,

Antony³

et al. 2022

J. Pure Appl. Microbiol.

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Antilisterial bacteriocin producing strain were isolated from milk samples and were subjected to 16S rRNA sequencing and found to be of genus Enterococcus faecium. The bacteriocin ALC102 were partially purified by Amberlite XAD-16 adsorption followed by column chromatography. The biofilm formation capacity of Listeria monocytogenes MTCC 657 were evaluated by tube method and CV binding assay. Biofilm formation on different abiotic substrates were also evaluated. Among three substrates stainless steel had a strong biofilm formation followed by glass and aluminum foil. From the results of biofilm eradication studies, the bacteriocin ALC102 showed almost similar activity of commercial bacteriocin nisin on all the substrates at 45°C, 30°C, 4°C and -20°C. Based on CBD® biofilm eradication assay, the eradication potential of ALC102 and nisin were found to be similar on high (45°C) and freezing (-20°C) temperatures. From the study, antilisterial bacteriocin ALC102 found to be able to inhibit the biofilm formed Listeria monocytogenes MTCC 657 at different temperatures and different incubation periods (24h, 48h and 72h). The biofilm eradication potential of antilisterial bacteriocin ALC102 was similar to nisin. Neither incubation temperature nor incubation period doesn’t altered the activity of the bacteriocin. So this bacteriocin can be considered as a potential competitor in food industry and we strongly recommend the use of this bacteriocin from Enterococcus faecium GRD AA in the food preservation industry to a higher temperature (45°C) to freezing temperature (-20°C).

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Section: Tube Methodsmentioning

confidence: 85%

Section: Tube Methodsmentioning

confidence: 99%