Effect of bevacizumab on acetic acid–induced ulcerative colitis in rats

Özsoy, Zeki; Özsoy, Şeyma; Gevrek, Fıkret; Demir, Emre; Benli, İsmail; Daldal, Emin; Yenidoğan, Erdinç

doi:10.1016/j.jss.2017.05.011

Cited by 19 publications

(12 citation statements)

References 36 publications

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“…MPO is a peroxidase enzyme most abundantly found in the azurophilic granules of neutrophils (Klebanoff, 2005). High MPO activity is a hallmark of intense infiltration of neutrophils, which may contribute to significant tissue damage (Ozsoy et al, 2017). In our study, Myr + AA-treated mice showed a significant decrease of colonic MPO activity when compared to the AA-alone treatment, suggesting an anti-inflammatory effect of Myr.…”

Section: Discussionsupporting

confidence: 55%

Protective effects of myristicin against ulcerative colitis induced by acetic acid in male mice

Badr

Amalki

Alfwuaires

et al. 2020

Food and Agricultural Immunology

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The present investigation aims to evaluate the prophylactic effects and mechanisms of Myr on experimental colitis model induced by acetic acid (AA) in mice. The obtained results showed that Myr treatment prior to induction of colitis significantly modulated the colonic oxidative stress by increasing the antioxidant enzymatic activities of catalse, superoxide dismutase, glutathione peroxidase and glutathione reductase, as well as by lowering the levels of lipid peroxidation. The treatment also significantly decreased the colonic tissue levels of inflammatory markers, including myeloperoxidase activity, tumour necrosis factor-alpha, interleukin-1 and interleukin-17. Besides, Myr significantly inhibited nuclear factor-kappa B (NF-κB) levels in AA-induced ulcerative colitis tissues. In the histological evaluation, the tissue damage scores in the mice colon were significantly suppressed with the administration of Myr + AA. Taken together, our findings suggested that Myr exerts marked protective effect against colonic inflammation through regulating antioxidative responses, NF-κB and subsequent pro-inflammatory cytokines production.

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Section: Discussionsupporting

confidence: 55%

Protective effects of myristicin against ulcerative colitis induced by acetic acid in male mice

Badr

Amalki

Alfwuaires

et al. 2020

Food and Agricultural Immunology

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“…MDA is a product of the lipid peroxidation that takes place as a consequence of colonic insult (Ozsoy et al, 2017). On the other hand, depletion of tissue GSH has been implicated in the development of UC (Shalkami et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Lycopene mitigates experimental colitis in rats by inhibiting oxidative stress-mediated inflammation and apoptosis

Hashem

Hussein

Senosi

2020

Benha Veterinary Medical Journal

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The potential protective and beneficial effects of Lycopene (LYC) in a rat model of acetic acid-induced ulcerative colitis (UC) were evaluated. Forty male albino rats were divided into five equal groups. Group I: (Control normal) rats received no drugs. Group II: (Early ulcerative colitis): rats received 2 ml (3% v/v) glacial acetic acid intra-colonially at 21 th day of experiment and sacrificed 3 days later. Group III:(Early ulcerative colitis + Lycopene protected) rats received Lycopene (10 mg/kg body weight/day, orally) for 21 successive days prior to acetic acid administration. Group IV: (Late ulcerative colitis) rats received acetic acid similar to group II for 3 successive days and sacrificed after 21 days. Group V: (Late ulcerative colitis+ Lycopene treated) rats first administered with acetic acid then after 3 days Lycopene was administered for 21successive days. A significant increase in L-Malondialdehyde (L-MDA) with marked decreases in reduced glutathione (GSH) level and Catalase (CAT) activity were observed in colon tissue of UC-induced rats. Additionally, a significant up-regulation of nuclear factor kappa B (NF-κB), caspase-3 and transforming growth factor-β1 (TGF-β1) and down-regulation of Interleukin-10 (IL-10) and Beta cell lymphoma-2 (Bcl-2) gene expression levels were observed in colon of UC induced rats. However, a significant depletion of colon tissue L-MDA and down-regulation of NF-κB, caspase-3 and TGF-β1 in addition to marked increases in GSH concentration and CAT activity and up-regulation of IL-10 and Bcl-2 were observed after LYC treatment. Lycopene has powerful antioxidants, anti-inflammatory and anti-apoptotic effects against ulcerative colitis.

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“…Zhang et al has assessed histological damage by using a combined score of inflammatory cell infiltration and mucosal damage [53]. Ozsoy et al has evaluated tissue damage only using tissue inflammation [54]. Overall, the evaluation standards of tissue damage are not uniform.…”

Section: Discussionmentioning

confidence: 99%

“…The colon was opened longitudinally and flushed with ice-cold normal saline. The intestinal segments were collected at a distance of 6 cm from anus and fixed in 4% paraformaldehyde for 24 h. The fixed samples were then embedded in paraffin wax, stained with H&E for histopathological analysis in accordance with previously published literature [52][53][54] with some modifications. The first step was to observe whether the transverse structure of the colon was intact (intact: 0; mucosa damage: 1; submucosa damage: 2; muscle damage: 3).…”

Section: Induction and Assessment Of Oxz-induced Colitismentioning

confidence: 99%

Improvement of Oxazolone-Induced Ulcerative Colitis in Rats Using Andrographolide

et al. 2019

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Ulcerative colitis (UC) is usually accompanied with symptoms of abdominal pain, diarrhea, and bloody stool, which impair the quality of life of patients. Previous studies have shown that Andrographis paniculata extracts, which have andrographolide (AND) as their main compound, can relieve UC symptoms in patients. The aim of the study was to investigate the alleviating effect of AND on UC using the oxazolone (OXZ)-induced UC rat model. A total of 66 healthy male Sprague Dawley rats were used to evaluate the efficacy and mechanism of AND on UC (n = 11 per group) and grouped into control, model, SASP (sulfasalazine, positive control group, 500 mg/kg), AND-L (40 mg/kg), AND-M (80 mg/kg), and AND-H (120 mg/kg). The colonic disease activity index (DAI), colon length, spleen coefficient, pathological damage, and inflammation-related cytokine and protein expression levels were used as indices for evaluation. Results showed that the AND groups had reduced DAI and mortality, and significantly improved colon length and spleen coefficient compared with the model group. Furthermore, OXZ-induced histological injury was relieved significantly after AND treatment due to an improved crypt structure and reduced infiltration of inflammatory cells. Moreover, AND inhibited myeloperoxidase (MPO) activity and the secretion of interleukin-4 (IL-4), IL-13, and tumor necrosis factor α (TNF-α). The results of the anti-inflammatory mechanism revealed that AND blocked the signal transduction by reducing IL-4/IL-13 specific binding to IL-4 receptor (IL-4R) and inhibiting the phosphorylation of the signal transducer and activator of transcription 6 (p-STAT6). In conclusion, aside from natural plants, AND may be a candidate ingredient for UC therapy.

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Effect of bevacizumab on acetic acid–induced ulcerative colitis in rats

Cited by 19 publications

References 36 publications

Protective effects of myristicin against ulcerative colitis induced by acetic acid in male mice

Protective effects of myristicin against ulcerative colitis induced by acetic acid in male mice

Lycopene mitigates experimental colitis in rats by inhibiting oxidative stress-mediated inflammation and apoptosis

Improvement of Oxazolone-Induced Ulcerative Colitis in Rats Using Andrographolide

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