Effect of bilateral lesions of nucleus accumbens on consummatory behaviour in Wistar rats

Mayannavar, Santhosh K.; Shiva, Rashmi Kaup; Aithal, Keerthi; Rama, Bhat; Ganaraja, B

doi:10.1016/j.jopr.2013.03.010

Cited by 2 publications

(3 citation statements)

References 28 publications

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“…From our previous study, we established that rats preferred to consume alcohol at 10% solution which has been confirmed in our own previous study. [ 33 ] Therefore in this study, we provided alcohol in this dilution following the infusion of drug into NAcc. We found a significant decrease in the alcohol intake in the hours immediately following the infusion of Orexin A antagonist.…”

Section: Discussionmentioning

confidence: 99%

Effect of Orexin A antagonist (SB-334867) infusion into the nucleus accumbens on consummatory behavior and alcohol preference in Wistar rats

et al. 2016

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Objective:Nucleus accumbens (NAcc) has a role in addiction and ingestive behavior. In order to assess orexinergic system involved in this, we infused Orexin A antagonist and assessed the effect on food intake fluid intake and alcohol preference in Wistar rats.Materials and Methods:Inbred Wistar rats (n = 54) were divided into control and experimental groups (low dose and high dose). Using stereotaxic method, guide cannula was set in place bilaterally to reach NAcc. Low dose (3 ng) and high dose (6 ng) of Orexin A antagonist (SB-334867) was infused, and the food consumption, water intake and alcohol intake, and two bottle free choice preference test for alcohol were carried out in the experimental group. The control group received saline infusion and rest of the methods followed were same. The measurements were carried out immediately after the infusion, at 1 h, 2 h, 4 h, and for the whole day and represented in the figure and tables.Results:A decrease in water intake observed immediately after the infusion in 1st h (P < 0.05) and 2nd h (P < 0.01), which was more in high dose group compared to low dose and controls. Alcohol intake was also following the same pattern. In two bottle free choice, rats did not show any specific preference to alcohol.Conclusion:There was dose dependent reduction in intake of food and fluids in treated rats. This suggested a possible role for orexinergic system in ingestive behavior. However, Orexin A may not have a role in modulation of alcohol addiction by the rewarding center NAcc.

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Section: Discussionmentioning

confidence: 99%

Effect of Orexin A antagonist (SB-334867) infusion into the nucleus accumbens on consummatory behavior and alcohol preference in Wistar rats

et al. 2016

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“…Stereotaxic surgical procedure and implantation of cannula was done according to our previously published work. 17,18,19 Micro infusion procedure Orexin B (30nanomol/µl) (Catalog no. 06262 Sigma Aldrich, St Louis, USA) was administered bilaterally into the specific nuclei in separate groups of rats.…”

Section: Surgical Procedures and Cannulationmentioning

confidence: 99%

“…20 TCS-OX2-29 (Catalog.No.3370; Tocris Bioscience, UK), a Orexin B antagonist, was dissolved in 0.9% saline and injected at a dose of 10 micrograms/µl in separate groups of rats. 17 Sham controls with the surgical procedure and injected with 0.9% saline. The infusion Orexin B and TCS-OX2-29 was performed according to the methodology published in the earlier work of current laboratory.…”

Section: Surgical Procedures and Cannulationmentioning

confidence: 99%

Role of Catecholamine Pathways in Action of Orexin B Induced Open Field Activity by Nucleus Accumbens in Wistar Rats

Ks¹,

Ganaraja

Mayannavar³

et al. 2018

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Aims:To elucidate whether exploratory behaviour changes following Orexin B infusion in NAc is mediated through catecholamines in male Wistar rats. Methods: Inbred male Wistar rats (n= 24) were divided into three groups. Control, Treated 1 (Orexin B) and Treated 2 (Orexin B antagonist) groups. Using stereotaxic method, guide cannula was set in place bilaterally to reach Nucleus Accumbens. Orexin B and its antagonist, TCS-OX2-29 were infused in separate groups of overnight fasted rats. Following open field activity, catecholamines (Dopamine, Adrenaline, and Noradrenaline) were estimated in brain tissue homogenate by ELISA. Data were expressed as mean±SEM (ANOVA; Student-Newman Keuls test,).p<0.05 were considered as statistically significant. Results: Orexin B infusion significantly increased noradrenaline levels in Nucleus accumbens when compared to controls. TCS-OX2-29 significantly decreased noradrenaline in Nucleus accumbens during open field exploration activity. Adrenaline was not altered significantly during open field activity. Conclusion: These results suggest that Orexin B, which plays a role in the regulation of motor and exploratory behaviour when infused in NAc mediates these actions through noradrenergic neurotransmission in Nucleus Accumbens.

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Effect of bilateral lesions of nucleus accumbens on consummatory behaviour in Wistar rats

Cited by 2 publications

References 28 publications

Effect of Orexin A antagonist (SB-334867) infusion into the nucleus accumbens on consummatory behavior and alcohol preference in Wistar rats

Effect of Orexin A antagonist (SB-334867) infusion into the nucleus accumbens on consummatory behavior and alcohol preference in Wistar rats

Role of Catecholamine Pathways in Action of Orexin B Induced Open Field Activity by Nucleus Accumbens in Wistar Rats

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