Effect of bile on growth and biofilm formation of non-typhoidal salmonella serovars isolated from seafood and poultry

Kotian, Akshatha; Aditya, Vankadari; Jazeela, Kadeeja; Karunasagar, Iddya; Deekshit, Vijaya Kumar

doi:10.1016/j.resmic.2020.06.002

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…Only for 14.5% of the studied S. enterica adding 3.0% of bile to the medium did not change the degree of biofilm formation. Our results are in opposition to the results obtained by Kotian et al [26]. They found that 3% bile increases biofilm formation by non-typhoidal salmonella serovars isolated from seafood and poultry [26].…”

Section: Discussioncontrasting

confidence: 99%

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Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains

et al. 2020

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Biofilm formed by S. enterica on the surface of gallstones or biomaterials promotes the development and spread of chronic infection. The aim of the study was to assess biofilm formation on the surface of polystyrene depending on nutritional conditions and the effect of 0.5, 1.0, and 2.0% glucose and 3.0% bile and sub-inhibitory concentrations of ampicillin on biofilm formation of S. enterica. Sixty-nine clinical strains of S. enterica isolated from feces (92.8%) and blood (7.2%) collected from patients (66.7%) and carriers (33.3%) were used in the study. Assessment of forming 24-h biofilm by these strains was performed on the surface of polystyrene 96-well plates at 37 °C. In this study, it was indicated that 1.0% glucose and 3.0% bovine bile inhibit biofilm formation. Biofilm formation was inhibited in all examined sub-MIC of ampicillin. Biofilm formation is varied in different conditions, depending on the serovar.

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Section: Discussioncontrasting

confidence: 99%

“…Our results are in opposition to the results obtained by Kotian et al [26]. They found that 3% bile increases biofilm formation by non-typhoidal salmonella serovars isolated from seafood and poultry [26]. Growth of Salmonella spp.…”

Section: Discussioncontrasting

confidence: 99%

Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains

et al. 2020

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“…Most of the clinical isolates were strong biofilm formers under in vitro gut conditions such as the presence of bile. This corroborates the report by Kotian et al (2020) who observed that majority of the seafood associated nontyphoidal Salmonella showed strong biofilm formation in the presence of bile (Kotian et al, 2020). Escherichia coli , being a gut commensal with some being pathogenic types, have evolved to survive in the presence of bile salts using bile as a signal to modulate the production of adhesive structures that further help in biofilm formation (Rossi, et al, 2018).…”

Section: Discussionsupporting

confidence: 92%

“…Additionally, as a survival strategy to overcome the unfavourable environment of the gut including the pressure of antibiotics, the bacteria form biofilms (Kotian et al, 2020). In this study, the biofilm‐forming ability was higher in clinical isolates than in environmental isolates.…”

Section: Discussionmentioning

confidence: 99%

Effect of ciprofloxacin and in vitro gut conditions on biofilm of Escherichia coli isolated from clinical and environmental sources

Aditya

Kotian

Saikrishnan

et al. 2021

J of Applied Microbiology

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Aim:This study aimed at characterizing the biofilm-forming ability of drug-resistant and sensitive Escherichia coli under in vitro gut conditions and in the presence of ciprofloxacin. Methods and Results: 153 E. coli isolates comprising 80 from clinical and 73 from environment source were studied for their ability to form biofilm under control and in vitro simulated gut conditions. The integrity of preformed biofilm on exposure to ciprofloxacin was assessed. Expression of biofilm-associated genes was analysed using qPCR. A high degree of resistance was observed in clinical isolates with a concomitant prevalence of bla TEM . Bile, pH and low temperature enabled the E. coli biofilm to resist the effect of ciprofloxacin. Clinical isolates of E. coli formed strong biofilms in in vitro gut conditions following exposure to high concentration of ciprofloxacin. The expression of biofilm genes varied between different gut conditions viz., presence of bile, pH and low temperature, included in this study. Conclusions:This study demonstrates the importance of papC and csgA for maintaining the biofilm integrity upon antibiotic exposure. Escherichia coli form biofilm as a survival strategy to adapt to the conditions in their environment irrespective of their drug resistance status. Significance and Impact of the Study:The study provides an understanding of the effect of different parameters of the gut conditions during infection and the effect of antibiotic on survival and biofilm-forming ability of clinical and environmental E. coli isolates. It further suggests that bacteria resort to biofilm formation as one of the mechanisms to adjust to alterations in gut conditions and once the biofilm is formed, it requires high concentration of ciprofloxacin to eradicate it.

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“…18 isolates were drug sensitive and the rest 11 isolates were resistant to only one antibiotic used in the study. Maximum sensitivity was seen towards chloramphenicol and cipro oxacin (91.37%) and maximum resistance was observed against nalidixic acid (50%) followed by co-trimoxazole (44.8 3%) as discussed in our previous study (Kotian et al 2020). Two isolates, Salmonella Weltevreden (SW9) and Salmonella Newport (SN36) that showed resistance to 4 (nalidixic acid, tetracycline, chloramphenicol and co-trimoxazole) and 5 (nalidixic acid, tetracycline, chloramphenicol, ampicillin and co-trimoxazole) different classes of drugs respectively were chosen for further study.…”

Section: Antibogram Analysis and Antibiotic Resistance Determinantssupporting

confidence: 67%

Survival, Virulence Gene Expression and Difference in the Drug Susceptibility of Non-typhoidal Salmonella in Gut Physiological Conditions

Kotian

Aditya

Sheikh

et al. 2021

Preprint

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Salmonella is one among the most versatile and resilient enteric pathogens that known to have developed various survival strategies within the host system. The ability of the bacteria to circumvent the physiological parameters as well as dodge the antimicrobial stress environment within the host is one of the most crucial steps in establishing an infection. With an alarming rise in multi-drug resistant serovars of non-typhoidal Salmonella and lack of vaccine for combatting the infections, behaviour of the bacteria in the presence of host physiological gut conditions (NaCl, high and low iron) and antibiotics will help in understanding the survival strategies as well as mechanisms of resistance. 59 non-typhoidal Salmonella serovars isolated from poultry and seafood were used in the study. The isolates were screened for their antibiotic susceptibility patterns. Two multi-drug resistant and two sensitive serovars were used for growth kinetics and virulence gene expression study. The results obtained revealed that despite similar resistance pattern, the effect of individual class of antibiotics on the growth of serovars varied. On contrary, no significant difference was observed in growth pattern on exposure to in vitro gut like experimental conditions. Nevertheless, the in-vitro gut conditions and exposure to antibiotics have drastically reduced the minimum inhibitory concentration of antibiotics in resistant strains. A first of its kind study that draws attention on the significant effect of antibiotics and gut physiological conditions on MIC and expression of virulence genes from Salmonella pathogenicity island (SPI) 1 and 2 between resistant and sensitive non typhoidal Salmonella serovars.

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Effect of bile on growth and biofilm formation of non-typhoidal salmonella serovars isolated from seafood and poultry

Cited by 10 publications

References 37 publications

Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains

Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains

Effect of ciprofloxacin and in vitro gut conditions on biofilm of Escherichia coli isolated from clinical and environmental sources

Survival, Virulence Gene Expression and Difference in the Drug Susceptibility of Non-typhoidal Salmonella in Gut Physiological Conditions

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