Effect of bile salt hydrolase-active<i>Lactobacillus plantarum</i>Y15 on high cholesterol diet induced hypercholesterolemic mice

Yin, Liu; Zheng, Shujuan; Cui, Jiale; Guo, Tingting; Zhang, Jingtao

doi:10.1080/19476337.2021.1914176

Cited by 9 publications

(3 citation statements)

References 51 publications

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“…Moreover, Lachnoclostridium and Desulfovibrio might be responsible for an increase in TC and LDL-C, respectively, in the serum and liver. Desulfovibrio and Lachnoclostridium were significantly more abundant in the MC group and decreased after the supplementation of L. plantarum Y15 [36], indicating that L. plantarum WLPL21 alleviated hypercholesterolemia by increasing the abundance of Allobaculum, Blautia, and Lactobacillus and reducing that of Lachnoclostridium and Desulfovibrio.…”

Section: Discussionmentioning

confidence: 93%

Alleviation Syndrome of High-Cholesterol-Diet-Induced Hypercholesterolemia in Mice by Intervention with Lactiplantibacillus plantarum WLPL21 via Regulation of Cholesterol Metabolism and Transportation as Well as Gut Microbiota

Zhao

Qiu

et al. 2023

Nutrients

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Probiotics are prospective for the prevention and treatment of cardiovascular diseases. Until now, systematic studies on the amelioration of hypercholesterolemia have been rare in terms of (cholesterol metabolism and transportation, reshaping of gut microbiota, as well as yielding SCFAs) intervention with lactic acid bacteria (LAB). In this study, strains of Lactiplantibacillus plantarum, WLPL21, WLPL72, and ZDY04, from fermented food and two combinations (Enterococcus faecium WEFA23 with L. plantarum WLPL21 and WLPL72) were compared for their effect on hypercholesterolemia. Comprehensively, with regard to the above aspects, L. plantarum WLPL21 showed the best mitigatory effect among all groups, which was revealed by decreasing total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels, upregulated cholesterol metabolism (Cyp27a1, Cyp7b1, Cyp7a1, and Cyp8b1) levels in the liver, cholesterol transportation (Abca1, Abcg5, and Abcg8) in the ileum or liver, and downregulated Npc1l1. Moreover, it reshaped the constitution of gut microbiota; specifically, the ratio of Firmicutes to Bacteroidetes (F/B) was downregulated; the relative abundance of Allobaculum, Blautia, and Lactobacillus was upregulated by 7.48–14.82-fold; and that of Lachnoclostridium and Desulfovibrio was then downregulated by 69.95% and 60.66%, respectively. In conclusion, L. plantarum WLPL21 improved cholesterol metabolism and transportation, as well as the abundance of gut microbiota, for alleviating high-cholesterol-diet-induced hypercholesterolemia.

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Section: Discussionmentioning

confidence: 93%

Alleviation Syndrome of High-Cholesterol-Diet-Induced Hypercholesterolemia in Mice by Intervention with Lactiplantibacillus plantarum WLPL21 via Regulation of Cholesterol Metabolism and Transportation as Well as Gut Microbiota

Zhao

Qiu

et al. 2023

Nutrients

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“…Activated cultures were inoculated into the medium with cholesterol and bile and isolate B11 has shown the highest cholesterol removal efficiency (88 ± 0.2%) with significant growth [Figure 3]. On similar notes, Liu et al [23] have studied cholesterol removal ability of Lactiplantibacillus plantarum Y15 through both in-vitro and in-vivo studies. They have observed high BSH activity in-vitro.…”

Section: Bacterial Growth and Cholesterol Removalmentioning

confidence: 98%

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

Chanpura¹,

Gupte²

2022

J App Biol biotech

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The present study is aimed to determine some important health beneficial properties of probiotic isolates such as cholesterol removal, β-galactosidase production, antioxidant, and anti-diabetic activity. Nine probiotic isolates were screened for Bile Salt Hydrolase (BSH) activity and potential BSH producers were selected for cholesterol removal study. Isolate B11 has shown significant cholesterol removal ability (88 ± 0.2%) after 24 h and this property was also analyzed with variable concentrations, time profile, with live, and dead cells as well as from egg yolk with simulation treatment. As probiotics improve lactose intolerance, all isolates were assessed for their lactose utilizing and β-galactosidase production ability where maximum enzyme activity was observed in case of isolate D25 (226 ± 0.30 Miller units). All the isolates were also assessed for their antioxidant potential using four different methods such as reducing power assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, 2,2-diphenyl-1-picrylhydrazyl assay, and superoxide dismutase activity. Out of nine isolates, isolate D25 and B11 have shown significant antioxidant property. Further, these isolates were also subjected to anti-diabetic study, where isolate D25 (89 ± 0.01%) and B11 (98 ± 0.30%) have shown high α-glucosidase inhibition which indicate their effective anti-diabetic activity. Hence, overall probiotic isolate D25 and B11 have shown significant health beneficial properties and they were further identified as Lactiplantibacillus plantarum and Pediococcus pentosaceus, respectively.

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“…Metagenome analysis based on the V4 region of the 16S rRNA gene of gut microbiota showed that L. plantarum Y15 supplementation elevated the diversity of the microbial community and contrasted the increase in Proteobacteria (13.54%), increasing the relative abundances of Lactobacillus , Bifidobacterium , Bacteroides , Clostridium , Prevotella , and Oscillospira . The expression levels of gene encoding FXR and SHP, that increase the catabolism of cholesterol and biosynthesis of bile acids by the α-hydroxylase CYP7A1 in the liver, were reduced by L. plantarum Y15 supplementation, while CYP7A1 gene was significantly upregulated, thus indicating that L. plantarum Y15 supplementation could lower cholesterol by upregulating cholesterol degradation genes [ 45 ].…”

Section: Lactiplanibacillus Plantarummentioning

confidence: 99%

In Vivo Functional Properties of Dairy Bacteria

et al. 2023

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This literature review aimed to collect investigations on the in vivo evidence for bacteria associated with fermented dairy foods to behave as probiotics with beneficial effects in the prevention and treatment of various diseases. All main bacterial groups commonly present in high numbers in fermented milks or cheeses were taken into account, namely starter lactic acid bacteria (SLAB) Lactobacillus delbrueckii subsp. bulgaricus and lactis, L. helveticus, Lactococcus lactis, Streptococcus thermophilus, non-starter LAB (NSLAB) Lacticaseibacillus spp., Lactiplantibacillus plantarum, dairy propionibacteria, and other less frequently encountered species. Only studies regarding strains of proven dairy origin were considered. Studies in animal models and clinical studies showed that dairy bacteria ameliorate symptoms of inflammatory bowel disease (IBD), mucositis, metabolic syndrome, aging and oxidative stress, cancer, bone diseases, atopic dermatitis, allergies, infections and damage caused by pollutants, mild stress, and depression. Immunomodulation and changes in the intestinal microbiota were the mechanisms most often involved in the observed effects. The results of the studies considered indicated that milk and dairy products are a rich source of beneficial bacteria that should be further exploited to the advantage of human and animal health.

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Effect of bile salt hydrolase-activeLactobacillus plantarumY15 on high cholesterol diet induced hypercholesterolemic mice

Cited by 9 publications

References 51 publications

Alleviation Syndrome of High-Cholesterol-Diet-Induced Hypercholesterolemia in Mice by Intervention with Lactiplantibacillus plantarum WLPL21 via Regulation of Cholesterol Metabolism and Transportation as Well as Gut Microbiota

Alleviation Syndrome of High-Cholesterol-Diet-Induced Hypercholesterolemia in Mice by Intervention with Lactiplantibacillus plantarum WLPL21 via Regulation of Cholesterol Metabolism and Transportation as Well as Gut Microbiota

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

In Vivo Functional Properties of Dairy Bacteria

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