Effect of Biscuits Enriched with Bilih Fish (Mystacoleucus padangensis) on Growth of Experimental Rats

Abstract

The research aimed to produce biscuits formulated with Bilih fish and to analyse the effect of Bilihfish-based biscuits on the growth of experimental rats. Six biscuit formulas were developed using three different amounts of Bilih fish flour (0 g, 20 g, and 30 g added to one formula dough) and two flavors (chocolate and vanilla). One biscuit formula was selected based on the best organoleptic test. Four treatments were applied to 24 male growing rats (21 day olds of the Spraque Dawley strain) for 28 days using a completely randomized design study. The treatments used were P1 (standard feed), P2 (standard feed + standard biscuits), P3 (standard feed + standard biscuits + pure omega-3 oil) and P4 (standard feed + the best formula biscuit). The parameters measured on rats were weight and tail length. The best formula biscuit based on the organoleptic test was the chocolate-flavor biscuits prepared with 20 g Bilih fish flour. The longest tail length was found in the P4 treatment (2,257±0.52 cm), which was significantly longer than the P2 (1.46±0.34 cm) and P1 (1.34±0.29 cm) treatments. No significant weight differences were found among the rats in all treatments. Hence, the chocolate-flavor biscuits formulated with the addition of 20 g Bilih fish flour increased the linear growth in experimental rats as shown by the significant difference in the tail lengths found in the treatment group.

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“…All rats were housed under controlled conditions at 24 °C, with a 12 h light/dark cycle. Body weight and tail length were measured every two weeks according to the Diana et al 2020 [ 13 ] method for a total feeding period of 8 weeks ( Figure 1 ). On day 56, after 12 h of fasting, all groups of rats were euthanized under ether anesthesia, blood was collected from the inferior vena cava, and serum was immediately separated.…”

NUGGET BERBASIS SUSU KERBAU DENGAN PENAMBAHAN IKAN BILIH (Mystacoleucus padangensis) SEBAGAI MAKANAN TAMBAHAN TUMBUH KEMBANG BALITA

“…Their studies indicated that the nutritional status of toddlers who were given biscuits made from snakehead fish flour and catfish flour had an impact on improving the nutritional status of the children 14,15 . The research conducted by Diana et al (2019) suggested that the development of a local product with the addition of 20 grams of bilih fish flour to one formula of chocolate-flavoured biscuit dough significantly influenced the increase in tail length, Y-Maze score, brain volume, and the number of hippocampal neuron cells (CA4 and DG) in experimental rats. Rats are the animals used for samples for toddlers 11,12,13,14,15 .…”

“…The research conducted by Diana et al (2019) suggested that the development of a local product with the addition of 20 grams of bilih fish flour to one formula of chocolate-flavoured biscuit dough significantly influenced the increase in tail length, Y-Maze score, brain volume, and the number of hippocampal neuron cells (CA4 and DG) in experimental rats. Rats are the animals used for samples for toddlers 11,12,13,14,15 .…”

“…Many researchers have examined the micronutrients contained in bilih fish. Per 100 grams, bilih fish contains zinc ranging from 4.76 to 17.33 milligrams, calcium ranging from 22 to 1128.05 milligrams, iron at 34.7 milligrams, vitamin A at 129.3 RE, vitamin E at 0.88 milligrams, phosphorus at 1.20 milligrams, omega-3 fatty acids at 0.553 grams, DHA at 0.237 grams, EPA at 0.207 grams, omega-6 fatty acids at 0.252 grams, and AA at 0.096 grams 9,10,11,12 . Bilih fish contains higher levels of zinc and calcium than other fish, such as mackerel, tuna, carp, tilapia, catfish, anchovies, walking catfish, snakehead, and bullet tuna 9. .…”