Effect of bladder outlet obstruction on detrusor smooth muscle cell: an in vitro study 1, 2 1Presented at the 6th Asian Congress of Urology (6th ACU), Kuala Lumpur, Malaysia, August 12–18, 2002 2This study was supported by a grant from National Natural Science Fund (No. 39970739), China, PRC.

Gong, Yanhong; Song, Bo; Jin, Xin; Xiong, Enqing

doi:10.1016/s0022-4804(03)00333-0

Cited by 18 publications

(4 citation statements)

References 28 publications

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“…Under abnormal or higher mechanical load, the smooth muscle cell will change its phenotypic type. The changed phenotypic type was able to proliferate [11]. In our study, the higher mechanical load induced the proliferation of bladder smooth muscle cell both in vitro and in vivo which could be inhibited by antimuscarinics.…”

Section: Discussionmentioning

confidence: 76%

“…Thus we think the proliferation of the bladder cells was an early biological change and early use of antimuscarinics may inhibit bladder hypertrophy caused by bladder cell proliferation. Another change of bladder cells in BOO was the ability to secrete extracellular matrix [11]. At 3 and 6 weeks, the percent collagen area was lower than that in the sham group.…”

Section: Discussionmentioning

confidence: 99%

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Protective Effects of Antimuscarinics on the Bladder Remodeling After Bladder Outlet Obstruction

Liu

Luo

Yang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Overactive bladder associated with bladder outlet obstruction (BOO) is a highly prevalent condition, which is usually treated with antimuscarinics. However, the potential effects of antimuscarinics on the structure and function of bladder have not been investigated thus far. Methods: Sprague-Dawley(R) rats accepted bladder neck obstruction surgery or sham surgery, and then received treatment of three different antimuscarinics (Solifenacin, Darifenacin, and Tolterodine) or vehicle. After 3, 6 and 12 weeks, the bladder function and structure were measured. The effect of antimuscarinics on cellular alteration in vitro was observed under mechanical stimulation. Bladder morphology were examined by immunohistochemistry, and the bladder function were investigated by cystometry and strip contractility test. The expression of muscarinic receptors and inflammatory cytokines were measured by PCR and Western blotting. Results: Here we demonstrate, both in vitro and in vivo, that antimuscarinics are protective regulators for the bladder structure and function. Antimuscarinics decrease the weight of bladders with BOO. Antimuscarinics improve the voiding parameter and enhance the contraction of bladder smooth muscle. The results also show that antimuscarinics inhibit the proliferation of bladder smooth muscle cells both in vivo and in vitro, it can reduce the collagen deposition and inflammatory cytokines in bladders with BOO. During this process, the expression of M2 and M3 receptors was altered by antimuscarinics. Conclusion: Antimuscarinics could reverse the structural and functional changes of BOO bladder wall at cellular and tissue level, and the alteration of M2 and M3 receptors may be involved in this biological process.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Protective Effects of Antimuscarinics on the Bladder Remodeling After Bladder Outlet Obstruction

Liu

Luo

Yang

et al. 2017

Cell Physiol Biochem

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“…Previous in vitro studies investigating the response of bladder SMCs to simulated pathological conditions have focused primarily on cyclic stretch of 2-D cultures of SMCs on a flexible substrate. 5,24 Since SMCs in vivo exist in 3-D environments surrounded by extracellular matrix proteins, in the present study, we developed an experimental setup to apply uni-axial sustained tension to cells in a 3-D environment. Although a number of similar studies have been reported using 3-D cultures of vascular SMC, very few have examined behaviors of bladder SMC in 3-D cultures.…”

Section: Discussionmentioning

confidence: 99%

“…19,22 Previous studies in the urology literature have also implicated a change in bladder SMC phenotype in response to cyclic stretch, which was demonstrated by decreased alpha-smooth muscle actin (a-SMA) organization, increased proliferation, and increased secretion of ECM proteins. 5,24 Although these studies provided some valuable information, they share a common method of twodimensional (2-D) culture of SMCs, which does not completely represent physiological conditions since the bladder cells in vivo exist as clusters surrounded by collagen sheaths in a three-dimensional (3-D) environment. 20 The overall goal of the present study, therefore, is to develop a 3-D culture of bladder SMCs which will allow investigation of the effects of mechanical stimuli on SMC phenotype within the 3-D environment.…”

Section: Introductionmentioning

confidence: 99%

Effect of Sustained Tension on Bladder Smooth Muscle Cells in Three-Dimensional Culture

2008

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Previous studies demonstrated that bladder cells respond to changes in their mechanical environments by exhibiting alterations in cellular functions, such as hypertrophy or fibrosis. In the present study, we hypothesize that changes in smooth muscle cell (SMC) behavior triggered by mechanical stimuli may represent a phenotypic shift between contractile and synthetic phenotypes. Using a custom-made device, rat bladder SMCs were cultured in three-dimensional (3-D) collagen gels and exposed to sustained tension. When compared to no-tension controls, SMCs exposed to tension exhibited significantly (p < 0.05) higher expression of alpha-smooth muscle actin (alpha-SMA), while cell population density was similar in both groups. In addition, both mean and median aspect ratios of SMCs in 3-D collagen constructs exposed to tension were significantly (p < 0.05) greater than those of cells cultured under no externally applied tension, indicating that there are more elongated, spindle-shaped cells in the tension group. These SMCs in 3-D cultures exposed to tension also exhibited cellular alignment along the direction of applied tension. Since contractile SMCs are known to exhibit greater expression of phenotypic marker proteins as well as a more elongated morphology, we concluded that sustained tension on cells is an important mechanical stimulus for maintenance of the contractile phenotype of bladder SMCs in vitro.

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The benefit of early mobilisation of tension-free vaginal tape in the treatment of post-operative voiding dysfunction

et al. 2009

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Effect of bladder outlet obstruction on detrusor smooth muscle cell: an in vitro study 1, 2 1Presented at the 6th Asian Congress of Urology (6th ACU), Kuala Lumpur, Malaysia, August 12–18, 2002 2This study was supported by a grant from National Natural Science Fund (No. 39970739), China, PRC.

Cited by 18 publications

References 28 publications

Protective Effects of Antimuscarinics on the Bladder Remodeling After Bladder Outlet Obstruction

Protective Effects of Antimuscarinics on the Bladder Remodeling After Bladder Outlet Obstruction

Effect of Sustained Tension on Bladder Smooth Muscle Cells in Three-Dimensional Culture

The benefit of early mobilisation of tension-free vaginal tape in the treatment of post-operative voiding dysfunction

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