Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ

Carvalho, Fabíola Christian Almeida de; Lucci, Carolina Madeira; Silva, José Roberto Viana; Andrade, Evelyn Rabelo; Báo, Sônia Nair; Figueiredo, José Ricardo de

doi:10.1016/s0378-4320(01)00085-9

Cited by 19 publications

(35 citation statements)

References 21 publications

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“…Some experiments have been conducted to evaluate the effects of storage temperatures and media on the quality of preantral follicles [1] and [2]. In general, researchers found storage temperatures of 4 and 20 °C to be more suitable than 39 °C (body temperature) for the preservation of the normal morphology of sheep [3] and goat [1], [2] and [4] preantral follicles in situ.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In general, researchers found storage temperatures of 4 and 20 °C to be more suitable than 39 °C (body temperature) for the preservation of the normal morphology of sheep [3] and goat [1], [2] and [4] preantral follicles in situ. Storage at 20 °C has proved suitable for the preservation of preantral follicles for up to 4 h [1] and [4] and 4 °C was effective for 12 or 24 h of preservation [1], [2] and [4], depending on the storage media. Media tested for the preservation of preantral follicles in ovarian pieces include saline solution (SS) [2] and [3], Braun-Collins solution [1], coconut water (CW) [1], [2] and [3], and M199 (with or without indole-3-acetic acid) [4].…”

Section: Introductionmentioning

confidence: 99%

“…Storage at 20 °C has proved suitable for the preservation of preantral follicles for up to 4 h [1] and [4] and 4 °C was effective for 12 or 24 h of preservation [1], [2] and [4], depending on the storage media. Media tested for the preservation of preantral follicles in ovarian pieces include saline solution (SS) [2] and [3], Braun-Collins solution [1], coconut water (CW) [1], [2] and [3], and M199 (with or without indole-3-acetic acid) [4]. However, all these studies were performed in small ruminant (sheep and goat) ovaries, and nothing is known about preserving preantral follicles in zebu cow ovaries.…”

Section: Introductionmentioning

confidence: 99%

“…The chosen temperatures (4 and 20 °C) were those that yielded the best results in previous studies [1], [2], [3] and [4], and the three storage times (6, 12, and 18 h) were taken at regular intervals of 6 h.…”

Section: Introductionmentioning

confidence: 99%

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Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

et al. 2004

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Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian folliclesThe maintenance of follicle quality during the transportation of ovaries is essential for the successful cryopreservation and in vitro development of preantral follicles. The objective of this study was to evaluate the effect of cooling ovarian tissue on the conservation of zebu cow preantral follicles. Ovarian pieces were immersed in saline or coconut water (CW) solutions and maintained at 4 or 20 °C for 6, 12, or 18 h. Preantral follicles were evaluated by histology and transmission electron microscopy. Storage of ovarian pieces at 20 °C for 12 or 18 h significantly reduced the percentage of morphologically normal follicles compared to controls. In contrast, conservation at 4 °C for up to 18 h and at 20 °C for up to 6 h kept the percentage of normal follicles similar to controls. However, the type of solution that the ovaries were immersed in had little effect on the results. Decreased cellular metabolism probably accounted for better preservation of preantral follicles at 4 °C. In conclusion, zebu cow ovaries were successfully stored at 4 °C for up to 18 h with no morphological damage to preantral follicles. However, at 20 °C, ovaries could only be stored for 6 h.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

et al. 2004

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Evidence of metabolic activity during low-temperature ovarian tissue preservation in different media

Vilela

Dolmans

Maruhashi

et al. 2020

J Assist Reprod Genet

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Purpose Although ovarian tissue transportation has been validated for up to 24 h, there is no standard protocol to date. We aimed to elucidate how existing media currently used for ovarian tissue transportation affect ovarian tissue metabolism and cell viability. Methods Cow ovarian fragments were immersed in 0.9% NaCl solution, IVF medium, Leibovitz 15 medium (L-15), or PBS for 1, 4, or 24 h at 4 °C. Media were analyzed for pH, lactate dehydrogenase (LDH) activity, and glucose, pyruvate, and lactate concentrations, while apoptosis was assessed by TUNEL assays in fixed fragments. Viability rates were assessed by flow cytometry (FACS). Results There were lower pH levels in NaCl at all time points compared with other media. LDH activity increased with time and was lowest in NaCl at 1 and 4 h. There was no significant difference in glucose levels, but a significant pyruvate decrease in L-15 and a significant lactate increase in all media. TUNEL showed apoptosis rates ranging from 0 to 5%. FACS showed a mean of 4% necrotic cells and 15-19% apoptotic cells after 1 h of incubation, but less than 1% necrotic cells and 2-6% apoptotic cells after 24 h in all media. Conclusion Our results indicate marked metabolic activity in ovarian tissue at 4 °C and suggest that cells use internal sources of energy, which may influence transplantation outcomes. This highlights the importance of better understanding whole tissue dynamics to develop a standard protocol for ovarian tissue transportation.

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Short-term preservation of canine preantral follicles: Effects of temperature, medium and time

Lopes

Santos

Celestino

et al. 2009

Animal Reproduction Science

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The use of the large pool of preantral follicles is a promising alternative to provide high numbers of fertilizable oocytes to reproductive biotechnology. This issue is particularly important to canids, since current rates of success of in vitro techniques using oocytes are very limited, and many species within this family are threatened by extinction. The aim of this study was to evaluate effects of temperature, medium and time on morphology and viability of canine preantral follicles during short-term preservation. Canine ovaries were cut into fragments which were incubated in 0.9% NaCl solution or in minimum essential medium (MEM) at 4, 20 or 38 °C for 2, 6, 12 or 24 h. Afterwards, preantral follicles were analyzed by histology, transmission electron microscopy and viability testing using trypan blue, calcein-AM and ethidium homodimer-1. Percentages of morphological normal and viable follicles were maintained similar to control (time 0 h) after incubation in 0.9% NaCl at 4 or 20 °C for up to 6 h and at 38 °C for 2 h. Using MEM, such preservation was possible for 12 h at 4 or 20 °C, and for 6 h at 38 °C. These results indicate that preservation of canine preantral follicles might be better accomplished through hypothermic (4 or 20 °C) storage in MEM, which ensures maintenance of morphology and viability for up to 12 h.

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Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ

Cited by 19 publications

References 21 publications

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

Evidence of metabolic activity during low-temperature ovarian tissue preservation in different media

Short-term preservation of canine preantral follicles: Effects of temperature, medium and time

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