Effect of breast-cancer metastasis suppressor 1 (BRMS1) on growth and metastasis of human gastric cancer cells in vivo

Jie, Zhigang; Chen, Xiaoping; Li, Zhengrong; Liu, Yi; Li, Yinliang; Zou, Xing; Yang, Huaicheng; Xiang, Deyu

doi:10.1007/s10330-011-0920-6

Cited by 1 publication

(4 citation statements)

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“…In a previous study, we have investigated the role of BRMS1 in GC invasion and metastasis in vitro 8. To further confirm this conclusion, we selected the nude mice for in vivo experiments and found that overexpression of BRMS1 did not affect the proliferation of GC cells compared with the control group ( P> 0.05) (Figure 5E and F).…”

Section: Resultsmentioning

confidence: 58%

“…As a tumor suppressor gene discovered over a decade ago, BRMS1 has been reported to suppress metastasis not only in breast cancer but also in hepatocellular carcinoma, non-small cell lung cancer, ovarian cancer and melanoma 6,7. Recently, we and others have demonstrated that BRMS1 expression was markedly stronger in the primary GC compared with metastasis GC and improve BRMS1 expression reduced the numbers of metastasis tumors in xenograft model 8,10. BRMS1 also can be recognized as a biomarker to predict the platinum-resistance for the patients of GC 35.…”

Section: Discussionmentioning

confidence: 95%

“…This molecule has been reported to suppress metastasis not only in breast cancer, but also in non-small cell lung cancer, ovarian cancer, melanoma, rectal cancer and so on 7. Detection BRMS1 expression in primary GC tissues and metastatic GC tissues by immunohistonchemistry (IHC) and construction of a xenograft model, we found that BRMS1 was markedly stronger in the primary tumor compared with metastasis gastric tumor, which can further suppress the metastasis of GC cells in nude mice but did not inhibit the growth of gastric tumors 8. For the past years, our team have found that the expression of BRMS1 was regulated by miR-125a-5p, which is low expressed in GC and further affects the invasion and metastasis of GC cells in vitro 9.…”

Section: Introductionmentioning

confidence: 86%

“…Female athymic BALB/c nude mice (4 weeks old) were used for the tumor formation assay as previously described 8. Female BALB/c nude mice (age, four weeks, weight, >15g) were purchased from Hunan SJA Laboratory Animal Co., Ltd (Changsha, China), which were randomly divided into four groups (n=5) to establish GC xenografts.…”

Section: Methodsmentioning

confidence: 99%

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<p>Epigenetics mechanisms mediate the miR-125a/BRMS1 axis to regulate invasion and metastasis in gastric cancer</p>

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Feng

et al. 2019

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Altered expression of breast cancer metastasis suppressor 1 (BRMS1), is a tumor suppressor, which is found in many types of cancers, including gastric cancer (GC), but the mechanism by which BRMS1 inhibits invasion and metastasis in GC is unknown. The aim of the study was to investigate the molecular mechanisms of miR-125a/BRMS1 in GC. Materials and methods: The expression of BRMS1 and miR-125a were detected by quantitative real-time PCR (qRT-PCR) and analyzed by bioinformatics. BSP and MSP were used to detecte the methylation status of miR-125a and BRMS1 which was treated by 5-Aza or not. Western Blot and qRT-PCR were used to analyze the expression of BRMS1 and EZH2. Transwell was performed to explore the invasion and metastasis ability of GC cells. The nude mice were used for the tumor formation assay. Results: BRMS1 may be regulated by copy number variation (CNV), methylation and miR-125a-5p. As one of the essential components of PRC2, EZH2 is an important regulatory factor resulting in the low expression of miR-125a. An epigenetic mechanism mediates the miR-125a/ BRMS1 axis to inhibit the invasion and metastasis of GC cells. In vivo experiments, it is also showed that BRMS1 is involved in invasion and metastasis but not the proliferation in GC. Conclusion: These studies shed light on the mechanism of BRMS1 inhibition of GC invasion and metastasis and the development of new drugs targeting the miR-125a/ BRMS1 axis, which will be a promising therapeutic strategy for GC and other human cancers.

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Section: Resultsmentioning

confidence: 58%