<p>Epigenetics mechanisms mediate the miR-125a/BRMS1 axis to regulate invasion and metastasis in gastric cancer</p>

Abstract

Altered expression of breast cancer metastasis suppressor 1 (BRMS1), is a tumor suppressor, which is found in many types of cancers, including gastric cancer (GC), but the mechanism by which BRMS1 inhibits invasion and metastasis in GC is unknown. The aim of the study was to investigate the molecular mechanisms of miR-125a/BRMS1 in GC. Materials and methods: The expression of BRMS1 and miR-125a were detected by quantitative real-time PCR (qRT-PCR) and analyzed by bioinformatics. BSP and MSP were used to detecte the methylation status of miR-125a and BRMS1 which was treated by 5-Aza or not. Western Blot and qRT-PCR were used to analyze the expression of BRMS1 and EZH2. Transwell was performed to explore the invasion and metastasis ability of GC cells. The nude mice were used for the tumor formation assay. Results: BRMS1 may be regulated by copy number variation (CNV), methylation and miR-125a-5p. As one of the essential components of PRC2, EZH2 is an important regulatory factor resulting in the low expression of miR-125a. An epigenetic mechanism mediates the miR-125a/ BRMS1 axis to inhibit the invasion and metastasis of GC cells. In vivo experiments, it is also showed that BRMS1 is involved in invasion and metastasis but not the proliferation in GC. Conclusion: These studies shed light on the mechanism of BRMS1 inhibition of GC invasion and metastasis and the development of new drugs targeting the miR-125a/ BRMS1 axis, which will be a promising therapeutic strategy for GC and other human cancers.

Circular RNA circBCBM1 promotes breast cancer brain metastasis by modulating miR-125a/BRD4 axis

“…MiRNAs are a class of non-coding small RNAs that post-transcriptionally regulate gene expression by binding to specific mRNA targets and promoting mRNA degradation and/or inhibiting mRNA translation 37 . Recently, miR-125a has been reported as a tumor suppressor that was significantly downregulated in multiple cancer types, such as non-small cell lung cancer 38 , gastric cancer 39 , bladder cancer 40 , etc. In our study, BRD4 was predicted as the candidate target gene of miR-125a by Targetscan and was verified by dual-luciferase reporter assay.…”

EZH2 is involved in psoriasis progression by impairing miR-125a-5p inhibition of SFMBT1 and leading to inhibition of the TGFβ/SMAD pathway

“…10 EZH2 is enriched in the miR-125a-5p promoter region which leads to the inhibition of miR-125a-5p expression. 9 Our RT-qPCR results demonstrated that the knockdown of EZH2 in HaCaT cells significantly increased the expression of miR-125a-5p (Figure 3A). The ChIP results illustrated that EZH2 was enriched in the miR-125a-5p promoter region in HaCaT cells.…”

“…10 EZH2 is enriched in the miR-125a-5p promoter region which leads to the inhibition of miR-125a-5p expression. 9 Our RT-qPCR results demonstrated that the knockdown of EZH2 in HaCaT cells significantly increased the expression of The results of the data were measurement data, which were presented as the mean ± standard deviation and analyzed by independent-sample t-test, N = 30.…”

Overexpression of NLRPs as Independent Indicator for Prognosis of Patients with Head and Neck Squamous Cell Carcinoma

“…In another study, upregulated miR-125a-5p was found to enhance the metastatic ability of HNSCC cell lines [30; 31]. In addition, miR-125a-5p has been identi ed as an important cancer treatment target in a series of cancers, including gastric cancer [32], cervical cancer [33], and lung cancer [34]. miR-199a-3p is not only expressed abnormally in a variety of cancers but also regulates ITGA3 to inhibit metastasis in head and neck cancer [35].…”