Effect of c<scp>DMEM</scp> media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation

Budanur, Damla Tuncer; Zibandeh, Noushin; Genç, Deniz; Gökalp, Muazzez; Kaşalı, Kamber; Akkoç, Tunç; Sepet, Elıf

doi:10.1111/edt.12396

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…HPDLCs osteogenesis is a key process of periodontitis treatment. Some chemical substances can induce this process, for example, ectoine (Tuncer Budanur et al, 2018), asiaticoside (Fitri, Pavasant, Chamni, & Sumrejkanchanakij, 2018), berberine (Liu et al, 2018), and so on. Physical and biological methods can also promote osteogenesis in hPDLCs, for example, high-power, red-light-emitting diode irradiation (Yamauchi, Taguchi, Kato, & Umeda, 2018), and DNA methylation profile (Ai et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Upregulation of long noncoding RNA MEG3 inhibits the osteogenic differentiation of periodontal ligament cells

Liu

Zeng

Miao³

et al. 2018

Journal Cellular Physiology

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Section: Discussionmentioning

confidence: 99%

Upregulation of long noncoding RNA MEG3 inhibits the osteogenic differentiation of periodontal ligament cells

Liu

Zeng

Miao³

et al. 2018

Journal Cellular Physiology

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“…Flow cytometry analysis declared that the cells showed positive expression for CD29, CD105, CD146, and CD73 markers and negative for CD3, CD4, and CD20 markers, which agree with the criteria of the International Society of Cellular Therapy [ 48 ]. Osteogenic, chondrogenic, and adipogenic differentiation procedures were performed on characterised DPSCs, and Alizarin red, Alcian blue, and oil red stains were used, respectively, to determine their differentiation into cell lines in accordance with previous studies [ 2 , 53 , 54 ]. Tarte et al [ 55 ] also used staining procedures to compare the proliferation and differentiation of SHEDs and PDLSCs, but used von Kossa staining instead of Alcian blue to determine chondrogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

TNF-α, IL-1B and IL-6 affect the differentiation ability of dental pulp stem cells

et al. 2023

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Background This in vitro study examined the effect of the inflammatory cytokines (tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6) on osteogenic, chondrogenic, and adipogenic differentiation of dental pulp stem cells (DPSCs) which have significant relevance in future regenerative therapies. Methods DPSCs were isolated from the impacted third molar dental pulp and determined with flow cytometry analysis. DPSCs were divided into into 5 main groups with 3 subdivisions for each group making a total of 15 groups. Experimental groups were stimulated with TNF-α, IL-1β, IL-6, and a combination of all three to undergo osteogenic, chondrogenic, and adipogenic differentiation protocols. Next, the differentiation of each group was examined with different staining procedures under a light microscope. Histological analysis of osteogenic, chondrogenic, and adipogenic differentiated pellets was assessed using a modified Bern score. Statistical significance determined using one-way analysis of variance, and correlations were assessed using Pearson’s test (two-tailed). Results Stimulation with inflammatory cytokines significantly inhibited the osteogenic, chondrogenic and adipogenic differentiation of DPSCs in terms of matrix and cell formation resulting in weak staining than the unstimulated groups with inflammatory cytokines. On contrary, the unstimulated groups of MSCs have shown to be highly proliferative ability in terms of osteogenic, chondrogenic, and adipogenic differentiation. Conclusions DPSCs have high osteogenic, chondrogenic, and adipogenic differentiation capabilities. Pretreatment with inflammatory cytokines decreases the differentiation ability in vitro, thus inhibiting tissue formation.

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“…When cells reached 70-80% con uence, they were detached with 0.25% trypsin-EDTA (Gibco, USA). Adherent cells cultured to the third passage were characterized and analyzed for speci c surface markers using ow cytometry [14].…”

Section: Methodsmentioning

confidence: 99%

“…Control antibodies were phycoerythrin-conjugated or uorescein isothiocyanateconjugated and allophycocyanin-conjugated mouse IgG1 and mouse IgG2 (BD Biosciences, San Diego, CA, USA). Flow cytometry results were analyzed using a Beckman Coulter Navios EX and the Kaluza software [14].…”

Section: Dental Folicle Derived Mesenchymal Stem Cell (Df-msc) Charac...mentioning

confidence: 99%

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Human Dental Mesenchymal Stem Cells Restorate Immune Response in Sera of Pemphigus Vulgaris Patients

Ozgen,

Duran,

Ergun

et al. 2024

Preprint

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Pemphigus is an IgG-mediated autoimmune condition characterized by autoantibodies targeting desmogleins, leading to acantholysis. Current treatments, including systemic corticosteroids and immunosuppressive drugs, are associated with significant adverse effects. Mesenchymal stem cells (MSCs) offer a promising alternative due to their immunomodulatory properties and low immunogenicity. This study evaluates the immunomodulatory effects of Dental Follicle Mesenchymal Stem Cells (DF-MSCs) on Pemphigus Vulgaris (PV) patients and healthy controls by examining T-cell proliferation, apoptosis, cytokine levels, and anti-desmoglein 1/3 IgG profiles. Twenty-one symptomatic PV patients and eleven healthy volunteers were included. DF-MSCs were isolated, characterized, and differentiated into osteocytes, adipocytes, and chondrocytes. Peripheral Blood Mononuclear Cells (PBMCs) were co-cultured with DF-MSCs, and various assays were conducted to evaluate T-cell proliferation, apoptosis, regulatory T cells, cytokine expression, and autoantibody levels. Results showed that DF-MSC co-cultures significantly reduced lymphocyte proliferation (43.58–16.27%), IL-4 (38.06 ng/L to 32.26 ng/L), TNF-α (32.45 ng/L to 29.41 ng/L), and DSG1 (3.29 ng/ml to 3.00 ng/ml) and DSG3 (262.40 ng/ml to 245.08 ng/ml) levels in PV patients. An increase in regulatory T cells (1.22–3.75%), IL-10 (47.46 pg/ml to 54.94 pg/ml), and IFN-γ (12.39 ng/ml to 19.70 ng/ml) was also observed. No significant changes were noted in healthy controls. These findings suggest that DF-MSCs could potentially offer a curative approach for treating pemphigus by restoring immune balance. However, further clinical trials are necessary to confirm their efficacy.

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Effect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation

Abstract: Ectoine added to cDMEM media, promoted cell survival plus osteogenic and fibroblastic differentiation of hPDLMSCs.

Cited by 7 publications

References 35 publications

Upregulation of long noncoding RNA MEG3 inhibits the osteogenic differentiation of periodontal ligament cells

Upregulation of long noncoding RNA MEG3 inhibits the osteogenic differentiation of periodontal ligament cells

TNF-α, IL-1B and IL-6 affect the differentiation ability of dental pulp stem cells

Human Dental Mesenchymal Stem Cells Restorate Immune Response in Sera of Pemphigus Vulgaris Patients

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