Effect of cadmium on polyribosome structure and function in mouse liver

Gamulin, Stjepan; Car, Nikica; Narancsik, P.

doi:10.1007/bf01922292

Cited by 24 publications

(9 citation statements)

References 8 publications

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“…This method was advantageous, in that it requires small quantities of tissue (< 15 mg) and has high accuracy and sensitivity. Our results (Table 3) indicate that levels in excess of 8,000 ,ug/g were attained in play some degree of cytotoxicity (4)(5)(6)(7)(8)(16)(17)(18)(19)(20). Lung inflammation and necrosis are typically seen in response to aerosols containing 10 mg/m3, causing a disruption in mucociliary function.…”

Section: Resultsmentioning

confidence: 81%

Cytotoxicity and Ciliostasis in Tracheal Explants Exposed to Cadmium Salts

Gabridge¹,

Meccoli²

1982

Environmental Health Perspectives

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Cadmium salts were examined for their biological effects on ciliated respiratory epithelium in hamster tracheal explants. Cadmium chloride and cadmium acetate both caused significant decreases in ciliary motion when tested at 100 ,uM and above. Reductions in relative ciliary activity were dose-dependent and were first demonstrable at 8-32 hr. The decreased ciliary motion was accompanied by decreases in two key metabolic compounds (ATP and dehydrogenase) which are normally associated with cell viability. Histopathological examination of cadmium-treated tissues showed an epithelium thinner than normal, with extensive vacuolization and few, if any, intact ciliated cells. The various biological effects exerted by cadmium are presented, along with potential mechanisms of pathogenesis for the observed ciliostasis and cytonecrosis. Decreases in adenosine triphosphate appear to play a critical role in the development of cadmium-related effects on cellular function and metabolism.

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Section: Resultsmentioning

confidence: 81%

Cytotoxicity and Ciliostasis in Tracheal Explants Exposed to Cadmium Salts

Gabridge¹,

Meccoli²

1982

Environmental Health Perspectives

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“…The possibility that the particulate fractions were contaminated by cytosol was eliminated because the Cd content per protein of these fractions did not change after extensive washing. Since several authors have reported morphological changes of subcellular organelles (10-12) and dysfunction of mitochondria or ribosomes (13,14), then any Cd located in the mitochondria and microsomes may be expected to be involved in the toxic effect.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it has been reported that oxidative phosphorylation by isolated mitochondria (13) and protein synthesis by isolated ribosomes (14) were depressed in Cd-fed rats. This suggests that the Cd, as located in subcellular organelles, may be a primary location for the subsequent toxic effect in tissues.…”

Section: Introductionmentioning

confidence: 99%

The chemical form of cadmium in subcellular fractions following cadmium exposure.

Waku¹

1984

Environ Health Perspect

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Wistar rats were given drinking water containing 250 ppm Cd for 12 months. After excision of the kidney and liver, the organs were subfractionated into nuclear, mitochondrial, microsomal and cytosol fractions, and the chemical forms of Cd in the subcelulur fractions were examined. Although approximately 90%o of the total Cd was present bi the cytosol, in the form of metallothionein, 3-5% was also present in the mitochondrial fraction and 5-7% in the microsomal fraction from both organs. By Sephadex G-75 gel ifitration, after solubilizing the particulate fractions with sodium deoxycholate, approximately 89%o of Cd in the microsomal fraction and 94% in the mitochondrial fraction eluted with the same retention time as that of metallothionein in both liver and kidney, while the remainder was found in a high molecular weight protein fraction. The Cd that eluted with the high molecular protein fraction may be involved in dysfunction in subcellular organelies.For estimating the toxicity of Cd associated with the high molecular weight fraction, rat liver microsomes and CdCI2 were mixed with 1% of sodium deoxycholate and the proteinCd complex produced was isolated by eluting with Sephadex G-75. This complex had a strong toxicity toward the alcohol dehydrogenase activity (SH-enzyme), and the Ki values of the Cd-protein complexes decreased with increased amount of Cd bound to the microsomal protein fraction. The above results suggest that loosely bound Cd increased in the case of higher Cd/protein and plays a toxic role in the living cells.

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“…which retain full activity. Cadmium added to the protein synthetic cell-free system derived from control animals has a biphasic effect, in low concentration (10"s M) it slightly stimulates, and in higher concentrations (3 -1 0 X 10~5 M) it inhibits the incorporation of a radioactively labelled amino acid into proteins (Gamulin et al, 1977). In the livers of rats treated with cadmium for 2 -3 weeks the activity both of polyribosome and cytosol fractions is diminished (Norton and Kench, 1977).…”

Section: Introductionmentioning

confidence: 99%

Effects of Cadmium on Polyribosome Sedimentation Pattern in Mouse Liver

Car¹,

Naranscik²,

Gamulin³

1979

Pathobiology

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Cadmium injected to mice provokes an acute disaggregation of hepatic polyribosomes. The effect appears within 15 min, it is maximal after 1 h and then gradually disappears. The reaggregation of polyribosomes takes place 6–12 h after injection. The disaggregation of polyribosomes is linear with log doses in a range of 7.25–20 µmol/kg CdCl₂. Cycloheximide pretreatment prevents the disaggregation of polyribosomes in the livers of cadmium-treated mice.

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Effect of cadmium on polyribosome structure and function in mouse liver

Cited by 24 publications

References 8 publications

Cytotoxicity and Ciliostasis in Tracheal Explants Exposed to Cadmium Salts

Cytotoxicity and Ciliostasis in Tracheal Explants Exposed to Cadmium Salts

The chemical form of cadmium in subcellular fractions following cadmium exposure.

Effects of Cadmium on Polyribosome Sedimentation Pattern in Mouse Liver

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