Effect of Calcium on E-Cadherin Expression in Breast-Tumor Cells

Vandewalle, B.; Révillion, Françoise; Doutrelant, Philippe; Granier, A; Lefèbvre, Jean Louis

doi:10.3892/ijo.4.1.181

Cited by 4 publications

(4 citation statements)

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“…Cell‐cell adhesion mediated by N‐cadherin is crucial for osteogenesis. N‐cadherin was expressed by osteoblasts during differentiation . Our study also showed that cellular spheroids formed on CS had the highest osteogenic potential.…”

Section: Discussionsupporting

confidence: 63%

Microwell Chips for Selection of Bio‐macromolecules that Increase the Differentiation Capacities of Mesenchymal Stem Cells

Hsu

Lee

2013

Macromolecular Bioscience

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Microwell chips (25 mm × 25 mm) are fabricated to select proper substrates for growing three-dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio-macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (≈10(5) ) are needed for each chip. The expression level of N-cadherin, a cell-adhesion molecule, is used as an indicator for cell-cell interactions. MSC spheroids expressing the highest N-cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio-macromolecules that enhance the differentiation potential of MSCs.

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Section: Discussionsupporting

confidence: 63%

Microwell Chips for Selection of Bio‐macromolecules that Increase the Differentiation Capacities of Mesenchymal Stem Cells

Hsu

Lee

2013

Macromolecular Bioscience

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“…With lower calcium concentration in the 1.5mM calcium concentration culture medium, the MCF-7 cells express lower levels of E-cadherins as compared to MCF-7 cells cultured in 1.7mM and 1.9mM calcium concentration culture medium [54].…”

Section: Enzyme Linked Immunosorbent Assay (Elisa) Resultsmentioning

confidence: 92%

“…In the study presented by B Vandewalle and group, MCF-7 cells were grown in low extracellular calcium concentration environment (0.4mM) and high extracellular calcium concentration environment (2.5mM). MCF-7 cells grown in the lower extracellular calcium concentration environment presented cells' shapes that were rounded and 3 times smaller than cells cultured in higher calcium concentration [54].…”

Section: Effects Of Calcium Concentration On Cellsmentioning

confidence: 88%

“…Calcium ions bind to cadherin to cause a conformation change to ensure structural stability and hence able to form cell-cell adhesions [39]. The concentration of calcium also affects the expression levels of E-cadherin present on surface of epithelial cells [40]. There are many different types of cadherins which differ in structure and functions.…”

Section: E-cadherinsmentioning

confidence: 99%

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Microfluidics modelling of interstitial flow through a tumor and the study of tumor cell under dielectrophoresis

Tay¹

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E-Cadherin (E-cad) expression in duct carcinoma in situ (DCIS) of the breast

et al. 1997

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E-cadherin (E-cad) is an epithelial cell-cell adhesion molecule whose loss or reduced expression is associated with a more invasive tumour phenotype. Ninety-six cases of screen detected pure ductal carcinoma in situ (DCIS) were analysed immunocytochemically for expression of E-cad using the HECD-1 mouse monoclonal antibody. The in situ component in each case was classified on the basis of cytonuclear grade, extent of necrosis, Van Nuys classification and a newly devised Cardiff classification. The amount of E-cad expression was assessed semi-quantitatively using an intensity distribution method. There is significantly more expression of E-cad in well-differentiated DCIS when compared with poorly differentiated DCIS and this finding is highly significant (P < 0.001) irrespective of the classification system used. These findings suggest that progressive loss of E-cad expression may occur at an early stage of breast cancer development.

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Effect of Calcium on E-Cadherin Expression in Breast-Tumor Cells

Cited by 4 publications

References 0 publications

Microwell Chips for Selection of Bio‐macromolecules that Increase the Differentiation Capacities of Mesenchymal Stem Cells

Microwell Chips for Selection of Bio‐macromolecules that Increase the Differentiation Capacities of Mesenchymal Stem Cells

Microfluidics modelling of interstitial flow through a tumor and the study of tumor cell under dielectrophoresis

E-Cadherin (E-cad) expression in duct carcinoma in situ (DCIS) of the breast

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