Effect of carbohydrate source and growth conditions on the production of lipoteichoic acid by Streptococcus mutans Ingbritt

Jacques, Nicholas A.; Hardy, Lyn; Campbell, L K; Knox, K. W.; Evans, J D; Wicken, A. J.

doi:10.1128/iai.26.3.1079-1087.1979

Cited by 43 publications

(41 citation statements)

References 27 publications

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“…Standard medium consisted of the lowmolecular-weight fraction of Trypticase (BBL Microbiology Systems, Cockeysville, Md. )-yeast extract medium that passed through Amicon hollow fiber filter HlPlO (21). Glucose or fructose was added to a final concentration of 2% for batch culture experiments or 0.5% for continuous-culture experiments, and the medium was then sterilized by membrane filtration (21).…”

Section: Methodsmentioning

confidence: 99%

Comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of Streptococcus sanguis G9B

Knox¹,

Hardy²,

Markevics³

et al. 1985

Infect Immun

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Streptococcus sanguis G9B was grown in continu6tis culture at different generation times and pH values in media containing either glucose or fructose and differing in the concentrations of Na+ and K'. The growth pH, carbohydrate, and cation concentration each affected the yield of organisms, their ability to adhere to saliva-coated hydroxyapatite beads, and their hydrophobicity, as measured by adhesion to hexadecane. There was no correlation between adhesion to saliva-coated hydroxyapatite beads and hydrophobicity, the values for hydrophobicity varying between 44 and 83% for organisms that adhered poorly and between 24 and 75% for those that adhered effectively. For organisms grown in batch culture at pH 6.0 or 7.0 there was similarly no correlation between adhesion and hydrophobicity. The growth conditions also had a considerable influence on the productibn of extracellular protein. The total amount was greater at pH 7.5 than at other pH values, and there were also differences in the individual components in response to changes in generation time, pH, carbohydrate source, and catidn concentration. Two protein bands were identified, namely, glucosyltransferase and protein P1 (also called antigen B or I/II). However, there was no correlation between a particular protein component and adhesion.

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Section: Methodsmentioning

confidence: 99%

Comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of Streptococcus sanguis G9B

Knox¹,

Hardy²,

Markevics³

et al. 1985

Infect Immun

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“…In strain NCTC 10302 the R-polysaccharide is not attached covalently to the cell wall, but is present as a capsule and in the culture fluid and is therefore better considered as a cell surfaceassociated component. In view of the previous observations on the effect of generation time on the production of other surface-associated components, namely, proteins and lipoteichoic acid (7)(8)(9)23), the variations in the amounts of capsular polysaccharide may not be surprising, although it should be noted that other studies found that growth rates did not influence exo- polysaccharide synthesis (10). Changing the carbohydrate source from glucose to fructose has also been shown to affect the production of lipoteichoic acid and certain proteins (7), but more relevant to the current study is the report that the production of extracellular polysaccharide by a strain of Actinomyces viscosus was much greater in glucose than in fructose (18).…”

Section: Discussionmentioning

confidence: 98%

“…A chemostat is particularly suitable for such a study as the influence of a parameter such as generation time, pH, or change of nutrient can be studied while other variables are controlled. By such means it has been shown, for example, that the production of the cell wall typing antigen of Streptococcus mutans Ingbritt is not affected by different growth conditions (17), whereas the amount of lipoteichoic acid can vary over a wide range (8). The production of lipoteichoic acid was enhanced markedly by a change of growth-limiting nutrient from glucose to fructose, an effect that could also be achieved in batch culture (17).…”

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confidence: 99%

Effect of growth conditions on production of rhamnose-containing cell wall and capsular polysaccharides by strains of Lactobacillus casei subsp. rhamnosus

Wicken¹,

Ayres²,

Campbell³

et al. 1983

J Bacteriol

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Strains of Lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing H-polysaccharide and a rhamnosecontaining R-polysaccharide, were examined for the effect of growth conditions on the production of these two components. In strain NCTC 6375, R-and Hpolysaccharides accounted for an estimated 44 and 20%6, respectively, of the cell wall for organisms grown in batch culture with glucose as the carbohydrate source. Growth on fructose-containing media reduced the amount of R-polysaccharide by approximately 50% without affecting the amount of H-polysaccharide. Subculture of fructose-grown organisms in glucose restored the original proportions of the two polysaccharides. Galactose-and sucrose-grown cells behaved similarly to glucose-grown cells with respect to polysaccharide production, whereas growth in rhamnose or ribose showed values close to those for fructosegrown cells. Continuous culture of strain NCTC 6375 for more than 100 generations showed a gradual and irreversible reduction of the R-polysaccharide to less than 5% of the cell wall and an increase of the H-polysaccharide to 40%o of the cell wall. Other type culture strains of L. casei subsp. rhamnosus, NCIB 7473 and ATCC 7469, behaved similarly in batch and continuous culture. In contrast, strains of L. casei subsp. rhamnosus isolated at the Institute of Dental Research showed phenotypic stability with respect to the relative proportions of R-and Hpolysaccharides in both batch and continuous culture. Changes in polysaccharide composition of type culture strains were also mirrored in changes in the immunogenicity of the two components and resistance to the rate of enzymic lysis of whole organisms. For L. casei subsp. rhamnosus strain NCTC 10302 the Rpolysaccharide is present entirely as capsular material. The amount of Rpolysaccharide produced was also markedly dependent on the carbohydrate component of the medium in batch culture and both dilution rate and nature of the limiting carbohydrate in continuous culture, varying over a 10-fold range, whereas the cell wall H-polysaccharide remained constant.

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“…The anti-L-cAg:Ab serum was examined for reactivity with the c polysaccharide and other glucose-containing polymers by using a passive hemagglutination assay as previously described (15). The additional polysaccharides included (i) RGP, prepared from S. mutans B13 (RGP/B13) (15), (ii) lipoteichoic acid, prepared from strain Ingbritt (LTA/Ingbritt) (7), and (iii) dextran T-10 purchased from Pharmacia Fine Chemicals. The purified c polysaccharide, RGP/B13, and dextran T-10 were esterified with palmitic acid according to the methods of Hammerling and Westphal (5) to facilitate sensitization of sheep erythrocytes for use in the passive hemagglutination assays.…”

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confidence: 99%

“…Although S. inltans is known to accumulate surface glucans and clump when grown in sucrose-supplemented batch culture, no cell clumping was noted, even microscopically, under chemostat conditions when sucrose was the limiting growth nutrient. Examination of antisera for reactivity with dextran by the passive hemagglutination assay pro- vided a sensitive means for detecting antibodies to surface-associated glucans in amounts that were not evident in earlier studies (7,8).…”

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confidence: 99%

Characterization of serological cross-reactivity between polysaccharide antigens of Streptococcus mutans serotypes c and d

Grossi¹,

Prakobphol²,

Linzer³

et al. 1983

Infect Immun

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Immunological assays with antisera prepared against purified Streptococcus mutans serotype c polysaccharide demonstrated that a cross-reacting determinant on c polysaccharide reacted with the wall-associated rhamnose-glucose polysaccharide from S. mutans serotype d. Studies with 60 antisera prepared against chemostat cultures of S. mutans Ingbritt (c) demonstrated that the rhamnoseglucose polysaccharide cross-reactive determinant was consistently expressed on c antigen under a variety of growth conditions.

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Effect of carbohydrate source and growth conditions on the production of lipoteichoic acid by Streptococcus mutans Ingbritt

Cited by 43 publications

References 27 publications

Comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of Streptococcus sanguis G9B

Comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of Streptococcus sanguis G9B

Effect of growth conditions on production of rhamnose-containing cell wall and capsular polysaccharides by strains of Lactobacillus casei subsp. rhamnosus

Characterization of serological cross-reactivity between polysaccharide antigens of Streptococcus mutans serotypes c and d

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