Effect of Carbon Dioxide and Oxygen on the Growth of Orange-Colored Streptococcus Bovis Isolated From Bovine Rumen

Abstract

The function of C02-related growth-promoting effect in the orangecolored Streptococcus bovis was investigated. In the defined medium, CO2 requirement was very large compared with that in natural media. Most of 14C02 incorporated was detected in the protein part. On the other hand, in the Polypepton-yeast extract medium, 14CO2 incorporation was extremely small and about 40 % of the incorporated 14C02 was found in the lipid part. Though S. bovis required only biotin as an essential factor among the vitamins, in the Polypepton medium, some of unsaturated fatty acids and oleate-containing compounds could replace not only biotin but also CO2 requirement, whereas, in the synthetic medium, these compounds could not replace CO2 requirement. This result suggests that CO2 takes part in synthesizing not only amino acids but also fatty acids. S. bovis is sensitive to oxygen, but CO2 or unsaturated fatty acids can be replaced by 02 in natural media.

Molecular cloning and expression of two alpha-amylase genes from Streptococcus bovis 148 in Escherichia coli

“…Streptococcus bovis 148 isolated from a bovine rumen produces a large amount of lactic acid from raw starch as well as soluble starch (7)(8)(9)(17)(18)(19). This characteristic is unique in lactic acid-producing bacteria.…”

Anaerobic Cocci from the Bovine Alimentary Tract, the Amino Acids of their Cell Wall Peptidoglycans and those of various Species of Anaerobic Streptococcus

“…(1969). Both the availability of CO, and the presence of a complex medium favour growth of this organism (Hayashi & Kitahara 1976). Carbon dioxide stimulated the growth of the strains used in the present study and they did not grow anaerobically in the absence of CO,.…”

Growth yield of an orange-colored Streptococcus bovis No. 148.

“…2). The procedure was the same as described in the previous paper (9). Glucose added as an energy source was completely consumed except for the case of oxygen-free N2 atmosphere.…”

“…PY medium was also used for the cultures of the reference strains. The procedures for maintaining the cultures and preparing inocula were the same as those previously reported (9 Glucose assay. Glucose was determined spectrophotometrically by using hexokinase (EC 2.7.1.2) and glucose-6-phosphate dehydrogenase (EC 1.1.1 .49)(JO).…”