Growth yield of an orange-colored Streptococcus bovis No. 148.

Hayashi, Toshiro; Kozaki, Michio

doi:10.2323/jgam.26.245

Cited by 11 publications

(2 citation statements)

References 8 publications

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“…Streptococcus bovis 148 isolated from a bovine rumen produces a large amount of lactic acid from raw starch as well as soluble starch (7)(8)(9)(17)(18)(19). This characteristic is unique in lactic acid-producing bacteria.…”

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Molecular cloning and expression of two alpha-amylase genes from Streptococcus bovis 148 in Escherichia coli

Satoh

Niimura

Uchimura

et al. 1993

Appl Environ Microbiol

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The ot-amylase genes of Streptococcus bovis 148 were cloned in Escherichia coli MC1061, using pBR322. The recombinant plasmids were classified into two groups on the basis of their restriction maps. Southern blot analysis did not show homology between the two types of ca-amylase genes, and the two cx-amylase genes existed on the chromosomal DNA of S. bovis 148. The enzymatic properties and N-terminal amino acid sequences of the two purified enzymes produced by the cloned E. coli strains were quite different from each other. Particularly, one ca-amylase (Amy I) was adsorbed on raw corn starch and hydrolyzed raw corn starch, and another (Amy II) was not adsorbed on raw corn starch and did not hydrolyze raw corn starch. Amy I was considered to be the same as the extracellular a-amylase of S. bovis 148 in raw starch adsorbability, ability to hydrolyze raw corn starch, enzymatic characteristics, N-terminal amino acid sequence, and mode of action on soluble starch. Amy II showed a unique pattern of oligosaccharide production from soluble starch compared with the extracellular ac-amylase of S. bovis 148. Amy II was suggested to be an intracellular a-amylase of S. bovis 148.

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confidence: 99%

Molecular cloning and expression of two alpha-amylase genes from Streptococcus bovis 148 in Escherichia coli

Satoh

Niimura

Uchimura

et al. 1993

Appl Environ Microbiol

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“…Clostridium sp. A2012 were grown on synthetic vitamin-mineral medium (Hayashi & Kozaki 1980) supplemented with 0.1% yeast extract and reduced by a mixture of 0.025% cysteine HCl and 0.025Oh Na2S.9H,O. A trace metal solution containing nitrilotriacetic acid (Clark & Holms 1976) was also added to prevent precipitation of insoluble salts in the medium.…”

Section: Streptococcus Bovis Es1 Strep Bouis Sbol Andmentioning

confidence: 99%

Stoichiometry of glucose and starch splitting by strains of amylolytic bacteria from the rumen and anaerobic digester

Marounek

Bartos

1986

Journal of Applied Bacteriology

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The stoichiometry of glucose and starch splitting by the amylolytic bacteria Streptococcus bovis, Selenomonas ruminantium, Butyrivibrio fibrisolvens, Eubacterium ruminantium and Clostridium sp. was followed. There were many differences in the ratios of metabolites and in growth yields, as well as in the cell composition, between the growth on glucose and starch. The bacteria employ different nutritional strategies with respect to both energy sources.

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