Background: Fragment reattachment is an excellent option for coronal fractures of permanent incisors when the fragment is available. The mode of storage of the fragment in different media and the reattachment time is a very important factor in the success of fragment reattachment. Aim: The aim of the study is to evaluate and compare the effects of time of the fragment in storage media and the influence of the storage media on the reattached tooth fragment. Methodology: Sixty sound incisors were taken, split into four groups of 15 specimens each. They were further subdivided into eight subgroups of seven specimens (A1, B1, C1, and D1) and eight specimens (A2, B2, C2, and D2), respectively. The teeth were sectioned, and the fragments were stored in Hanks' balanced salt solution (HBSS) (group A1 and B2), GC Tooth Mousse (group B1 and B2), GC Tooth Mousse Plus (group C1 and C2), and nano-hydroxyapatite (nHA)-nfused dentifrice (group D1 and D2) for 12 and 24 hours, respectively. Fragments were reattached using the simple reattachment technique with flowable composite resin. The compressive strength was measured using a universal strength-testing machine. Result: Significant difference was found among group A vs B, group A vs C, and group A vs D at 24 hours. Insignificant differences were noted in the 12 hours group. Conclusion: Highest fracture resistance was seen in group D2, followed by B2,