Effect of cellulose physical characteristics, especially the water sorption value, on the efficiency of its hydrolysis catalyzed by free or immobilized cellulase

Ogeda, Thais Lucy; Silva, Igor Benedetti e; Fidale, Ludmila de Carvalho; Seoud, Omar A. El; Petri, Denise Freitas Siqueira

doi:10.1016/j.jbiotec.2011.11.018

Cited by 43 publications

(18 citation statements)

References 36 publications

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“…The PB design did provide the most adequate conditions for enzymatic hydrolysis, the Box-Behnken design best evaluated the results. Cellulase-enzyme complex is composed of endo b-1,4 glucanases, exo b-1,4 glucanases (cellobiohydrolases) and b-1,4 glucosidases (Ogeda et al 2012;Kuhad et al 2016). The third component of cellulase has great importance in the process because is responsible for effective glucoserelease.…”

Section: Selection Of Hydrolysis Parameters and Variablesmentioning

confidence: 99%

Study of enzymatic saccharification of Agave leaves biomass to yield fermentable sugars

et al. 2017

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Agave is a good source of polysaccharides for the production of fermentable sugars as sustainable bioenergy feedstock solutions for semi-arid and arid lands. This plant grows in arid areas, which correspond to a large territory in northern Mexico. Having lignocellulose as the polysaccharide of interest, the information for the enzymatic saccharification of this kind of material is limited. Agave cell walls have a unique recalcitrant nature, but having a high cellulose content, makes this plant material an interesting research subject. In this work, acidic, alkaline and aqueous pretreatments were evaluated to generate a biomass rich in cellulose. The saccharification of pretreated Agave leaves-residue was evaluated under experimental designs to identify the most suitable conditions for enzymatic hydrolysis. Maximum value obtained was 31% glucose, which further increased to 41.4% at extended hydrolysis time of 96 h. The highest cellulose-saccharification reached was up to 61.81%, making Agave atrovirens an alternative for bioethanol production in its geographical area of cultivation.

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Section: Selection Of Hydrolysis Parameters and Variablesmentioning

confidence: 99%

Study of enzymatic saccharification of Agave leaves biomass to yield fermentable sugars

et al. 2017

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“…Conversion of cellulose during the degradation of biomass residues and agricultural waste and products has been extensively studied in the context of biofuel production [1–3]. Other sources of lignocellulosic materials, such as waste, are currently emerging as attractive options for biorefinery based on anaerobic digestion [4–6].…”

Section: Introductionmentioning

confidence: 99%

Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties

Badalato¹,

Guillot

Sabarly³

et al. 2017

PLoS ONE

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Lignocellulosic materials from municipal solid waste emerge as attractive resources for anaerobic digestion biorefinery. To increase the knowledge required for establishing efficient bioprocesses, dynamics of batch fermentation by the cellulolytic bacterium Ruminiclostridium cellulolyticum were compared using three cellulosic materials, paper handkerchief, cotton discs and Whatman filter paper. Fermentation of paper handkerchief occurred the fastest and resulted in a specific metabolic profile: it resulted in the lowest acetate-to-lactate and acetate-to-ethanol ratios. By shotgun proteomic analyses of paper handkerchief and Whatman paper incubations, 151 proteins with significantly different levels were detected, including 20 of the 65 cellulosomal components, 8 non-cellulosomal CAZymes and 44 distinct extracytoplasmic proteins. Consistent with the specific metabolic profile observed, many enzymes from the central carbon catabolic pathways had higher levels in paper handkerchief incubations. Among the quantified CAZymes and cellulosomal components, 10 endoglucanases mainly from the GH9 families and 7 other cellulosomal subunits had lower levels in paper handkerchief incubations. An in-depth characterization of the materials used showed that the lower levels of endoglucanases in paper handkerchief incubations could hypothetically result from its lower crystallinity index (50%) and degree of polymerization (970). By contrast, the higher hemicellulose rate in paper handkerchief (13.87%) did not result in the enhanced expression of enzyme with xylanase as primary activity, including enzymes from the “xyl-doc” cluster. It suggests the absence, in this material, of molecular structures that specifically lead to xylanase induction. The integrated approach developed in this work shows that subtle differences among cellulosic materials regarding chemical and structural characteristics have significant effects on expressed bacterial functions, in particular the cellulolysis machinery, resulting in different metabolic patterns and degradation dynamics.

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“…Some other factors may also affect the ethanol yield. Physical properties and cellulose microstructure are among the potential factors that affect enzymatic hydrolysis (Ogeda et al 2012). Furthermore, Avicel was purchased in dried form.…”

Section: Simultaneous Saccharification and Fermentation By F Venenatmentioning

confidence: 99%

Bioconversion of pretreated wheat straw to ethanol by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334 using simultaneous saccharification and fermentation

Yudianto¹,

Nainggolan²,

Millati

et al. 2019

Biodiversitas

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Abstract. Yudianto D, Nainggolan EA, Millati R, Hidayat C, Lennartsson P, Taherzadeh MJ, Niklasson C. 2019. Bioconversion of pretreated wheat straw to ethanol by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334 using simultaneous saccharification and fermentation. 20: 2229-2235. Fractions of sulfuric acid-pretreated wheat straw, i.e. solid, liquid, and a mixture of liquid and solid were used as substrates in simultaneous saccharification and fermentation (SSF) process to produce ethanol. The bioconversion was performed by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334. The highest ethanol yields from solid, liquid and a mixture of solid and liquid fractions by M. purpureus CBS 109.07 were 0.36, 0.41, and 0.37 g/g glucose, respectively. The corresponding values by F. venenatum ATCC 20334 were 0.21, 0.54, 0.35 g/g glucose, respectively.

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Effect of cellulose physical characteristics, especially the water sorption value, on the efficiency of its hydrolysis catalyzed by free or immobilized cellulase

Cited by 43 publications

References 36 publications

Study of enzymatic saccharification of Agave leaves biomass to yield fermentable sugars

Study of enzymatic saccharification of Agave leaves biomass to yield fermentable sugars

Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties

Bioconversion of pretreated wheat straw to ethanol by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334 using simultaneous saccharification and fermentation

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