Effect of centrifugation and sugar supplementation on the semen cryopreservation of captive collared peccaries (Tayassu tajacu)

Castelo, T.S.; Bezerra, Francisco Silvestre Brilhante; Lima, Gabriela Liberalino; Alves, Heron M.; Oliveira, Isabelle Rayanne Sousa de; Santos, E.A.A.; Peixoto, Gislayne Christianne Xavier; Silva, Alexandre Rodrigues

doi:10.1016/j.cryobiol.2010.09.005

Cited by 29 publications

(33 citation statements)

References 28 publications

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“…Date contains a variety of nutrients such as carbohydrate, protein, antioxidant, and vitamin C that might act as spermatozoa protectants. Sugar contents like sucrose (Rizal et al, 2007), glucose, fructose, and others (Castelo et al, 2010) in extender serve as extracellular cryoprotectants that might protect plasma membrane from damage during cryopreservation (Mukminat et al, 2014;Rizal et al, 2007). Karp (2010) stated that carbohydrate in a cellular environment can be associated with lipid or protein of plasma membrane of cells.…”

Section: Fresh Semen Qualitymentioning

confidence: 99%

Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

Dwitya

Eriani

Saputra³

et al. 2019

J Bio Bio Edu

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Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.

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Section: Fresh Semen Qualitymentioning

confidence: 99%

Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

Dwitya

Eriani

Saputra³

et al. 2019

J Bio Bio Edu

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“…The stimulatory cycle comprised of 10 stimuli in each voltage, starting from 5-V, followed by voltage increases in steps of 1-V up to 12-V. Each electrical stimulus lasted for 3s with intermittent breaks of 2s. The stimuli were maintained for a duration of 10 min from the beginning of the procedure (Castelo et al 2010). The electroejaculator probe measured 15cm (length) and 1.3cm (diameter); and 12cm were inserted into the rectum of the male.…”

Section: Methodsmentioning

confidence: 99%

“…Several staining methods such as the brome-phenol blue (Toniolli et al 2010), the eosin-nigrosin (Moraes et al 2010), the diff-quick test (Kim et al 2011), the Cerovský (Fridrichová et al 2011), and the Williams-modified (Am-in et al 2011) are reported for sperm morphology analysis in the swine, that is the domestic species more closely related to the collared peccaries (Cavalcante-Filho et al 1998). In these last ones, only the eosin-nigrosin (Kahwage et al 2010) and the Bengal rose (Castelo et al 2010) staining are described for sperm evaluation, but comparisons among techniques are necessary in order to identify the most suitable for the species.…”

Section: Introductionmentioning

confidence: 99%

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

et al. 2013

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Pesq. Vet. Bras. 33 (7) The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.

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“…Recently, Castelo et al. (,b) reported the first success in cryopreserving the collared peccary semen using Tris‐based extenders added of egg yolk (20%) and glycerol (3%). This protocol provided 37% motile sperm after thawing.…”

Section: Introductionmentioning

confidence: 99%

“…Since that only 3% glycerol was previously tested for collared peccaries semen (Castelo et al. ,b), the search for other concentrations that provide a better cryoprotection is justified to improve the protocol. Based on the fact that glycerol concentrations as higher as 6% are currently used for the semen freezing in domestic animals (Silva et al.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Different Glycerol and Egg Yolk Concentrations Added toTris‐based Extender for the Collared Peccaries (Tayassu tajacu) Semen Freezing

Alves

Oliveira

Castelo

et al. 2012

Reprod Domestic Animals

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This study aimed to evaluate various concentrations of egg yolk (5, 10, or 20%) in combination with different concentrations of glycerol (3% or 6%) added to a Tris-based extender on the post-thaw characteristics of sperm obtained from Tayassu tajacu. For this purpose, semen from 10 sexually male mature collared peccaries was collected by electroejaculation and evaluated for sperm motility, vigour, viability, morphology and functional membrane integrity. The ejaculates were initially extended in Tris-fructose plus egg yolk (5%, 10% or 20%). After cooling, the semen was added to Tris-egg yolk plus glycerol (6% or 12%), resulting in a final concentration of 3% or 6% glycerol of the extender. Straws were frozen using liquid nitrogen and thawed in a water bath at 37°C for 30 s. The frozen-thawed semen was evaluated as reported for fresh semen. After thawing, a significant decrease was verified for sperm motility and vigour, for all the samples in comparison with fresh semen. However, no differences were evidenced among treatments for any sperm characteristics evaluated (p > 0.05), except for the combination between 10% egg yolk and 6% glycerol, which provided the worst preservation of functional membrane integrity (p < 0.05). The interactions between higher concentrations of egg yolk (20%) and glycerol (6%) and also between lower concentrations of the same substances (5% egg yolk and 3% glycerol) added to the Tris-based extender negatively affected the preservation of the normal sperm morphology after thawing (p < 0.05). In conclusion, the use of Tris-based extender added to 10% or 20% egg yolk plus 3% glycerol is recommended for effective sperm cryopreservation in collared peccaries.

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Effect of centrifugation and sugar supplementation on the semen cryopreservation of captive collared peccaries (Tayassu tajacu)

Cited by 29 publications

References 28 publications

Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

Comparison of Different Glycerol and Egg Yolk Concentrations Added toTris‐based Extender for the Collared Peccaries (Tayassu tajacu) Semen Freezing

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