Effect of chaotropic mobile phase additives on retention behaviour of beta-blockers on various reversed-phase high-performance liquid chromatography columns

Hashem, Hisham; Jira, Thomas

doi:10.1016/j.chroma.2006.07.074

Cited by 29 publications

(18 citation statements)

References 22 publications

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“…When the competition is dominant, retention decreases, whereas when the ion pair formation is dominant, it increases (Machida , 1999). These findings were further substantiated by Hashem and Jira (2006) for stationary phases containing calixarene cavities. Dai and Carr (2005) also pointed out that the retention of basic substances on conventional reversed phases can increase, decrease or effectively remain constant as the concentration of the mobile phase additives is increased.…”

Section: Methods Developmentsupporting

confidence: 64%

LC‐MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study

Ramisetti

Sravan

2014

Biomedical Chromatography

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A simple and selective polar ionic liquid chromatography-tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as a chiral stationary phase using 2.5 mm ammonium formate in 100% methanol as a mobile phase in an isocratic mode of elution at a flow rate of 1.0 mL/min. The analytes were extracted from rat plasma by precipitating the proteins with acetonitrile. The developed method exhibited a linear dynamic range over 0.5-500 ng/mL in rat plasma for both enantiomers. The method was successfully applied to study the pharmacokinetics after a single dose by oral administration of 10 mg/kg of cinacalcet enantiomers to healthy male Wistar rats.

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Section: Methods Developmentsupporting

confidence: 64%

LC‐MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study

Ramisetti

Sravan

2014

Biomedical Chromatography

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“…Delamoye et al, 2004) applied gradient elution of acetonitrile-phosphate buffer pH 3.8 to analyze 13 β-blockers on C 18 column. The effect of chaotropic counter anion in buffered mobile phase and the role of organic modifier (methanol, acetonitrile) type on the retention behavior of some β-blockers has been also investigated (Hashem and Jira, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of chemically bonded stationary phases and mobile phase composition on β‐blockers retention in RP‐HPLC

Buszewski

Welerowicz

Kowalkowski

2008

Biomedical Chromatography

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The effects of stationary and mobile phase on retention of 18 beta-adrenolytic drugs (beta-blockers) have been studied. Four 'deactivated surface' stationary phases (polar-embedded or end-capped) were examined. Special attention was drawn to the cholesterolic (SG-CHOL) and alkylamide (SG-AP) stationary phases, and their application for analysis of the compounds. The retention of analyzed substances was also examined in terms of mobile phase composition. Sixteen different configurations of mobile phases were prepared, all based on methanol and acetonitrile with ammonium acetate and ammonium formate. The difference in retention between ammonium formate and acetate water solutions, and peak shape changes related to the addition of triethylamine (TEA), were investigated. Principal component analysis was used to find the similarities between stationary phases. Polar-embedded phases synthesized on the same sorbent possess very similar properties. All phases based on silica gel compared with the monolithic column also showed similarities in retention of beta-blockers. The addition of TEA to the mobile phase did not influence strongly the retention, and analysis of asymmetry factors showed only a little peak broadening for a few compounds on the monolithic column.

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“…In the Hofmeister series, chaotropic ions are those having large size and low charge, such as the anions SCN 2 , ClO 4 …”

Section: Introductionmentioning

confidence: 99%

“…Ammonium sulfate is the classic example of an agent that causes proteins to separate from solution, because the chaotropic ions expose the hydrophobic domain of the protein and then like-to-like interactions enlarge the protein size, causing precipitation. Hashem and Jira [4] observed the satisfactory separation of protonated beta-blockers when using chaotropic anions as mobile phase additives for HPLC. Adding chaotropic additives provided a significant change in the resolution without the need to vary the column type or add an ion-pairing reagent.…”

mentioning

confidence: 98%

“…When a hydrophobic compound is dissolved in an aqueous solution, hydrated molecules gather around it, forming a "clathrate" to isolate the hydrophobic domain; intermolecular hydrogen bonds nearby the clathrate stabilize the hydrophobic compound [3]. Adding chaotropic ions disrupts the solvation shell of the analyte, increasing its apparent hydrophobicity [4]. The phenomenon of decreasing the structure of water and increasing the opportunity for hydrophobic compounds to interact with other ions to form complexes is called the chaotropic effect [5].…”

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confidence: 99%

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Chaotropic salts: Novel modifiers for the capillary electrophoretic analysis of benzodiazepines

Lan

Lin

et al. 2008

Electrophoresis

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This paper describes a CE method for analyzing benzodiazepines using the chaotropic salts lithium trifluoromethanesulfonate (LiOTf), lithium hexafluorophosphate (LiPF(6)), and lithium bis(trifluoromethanesulfonyl)imide (LiNTf(2)) as modifiers in the running buffer. Although adequate resolution of seven benzodiazepine analytes occurred under the influence of each of the chaotropic anions, the separation efficiency was highest when bis(trifluoromethanesulfonyl)imide (Tf(2)N(-)) was the modifier. We applied affinity CE in conjunction with linear analysis to determine the association constants for the formation of complexes between the Tf(2)N(-) anion and the benzodiazepines. According to the estimated Gibbs free energies, the interactions between this chaotropic anion and the benzodiazepines were either ion-dipole or ion-induced dipole interactions. Adding chaotropic salts as modifiers into CE buffers is a simple and reproducible technique for separating benzodiazepines.

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Effect of chaotropic mobile phase additives on retention behaviour of beta-blockers on various reversed-phase high-performance liquid chromatography columns

Cited by 29 publications

References 22 publications

LC‐MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study

LC‐MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study

Effect of chemically bonded stationary phases and mobile phase composition on β‐blockers retention in RP‐HPLC

Chaotropic salts: Novel modifiers for the capillary electrophoretic analysis of benzodiazepines

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