Effect of Chinese Herb Extract on the Formation of Triterpenoids in Shake-Flask Cultures of Antrodia cinnamomea

Wei, Te; Yang, Ning; Yang, Fan

doi:10.4172/2155-9821.1000307

Cited by 2 publications

(3 citation statements)

References 27 publications

(38 reference statements)

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Section: Discussionmentioning

confidence: 95%

“…Previous studies have reported the stimulatory activity of camphorwood extracts on the production of bioactive compounds, such as polysaccharides, antroquinonol, and antrodin C in A. camphorata, 23,24 but the underlying mechanism of the C. kanehirae-stimulated growth and metabolism of A. camphorata remains unknown. This study demonstrated the metabolic network of terpenoids in the A. camphorata mycelia via metabolomic analysis (Figure 4).…”

Section: ■ Discussionmentioning

confidence: 99%

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Characterization of Cinnamomum kanehirae Extract-Stimulated Triterpenoids Synthesis in Submerged Fermentation of Antrodia camphorata via Untargeted Metabolomics

Luo

Lü

et al. 2023

J. Agric. Food Chem.

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The underlying mechanisms of Cinnamomum kanehirae-stimulated growth and metabolism of Antrodia camphorata remain unknown. Herein, we first observed that the methanol extract of C. kanehirae trunk (MECK) (2 g/L) showed a potent stimulatory effect on A. camphorata triterpenoids production (115.6 mg/L). Second, MECK treatment considerably increased the category and abundance of many secondary metabolites in the mycelia. We identified 93 terpenoids (8 newly formed and 49 upregulated) in the MECK-treated mycelia, wherein 21 terpenoids were the same as those in the fruiting bodies. Third, 42 out of the 93 terpenoids were annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, mainly involving monoterpenoids and diterpenoids syntheses. Finally, 27 monoterpenes and 16 sesquiterpenes were detected in the MECK, and the two terpenoids with the highest abundance (linalool and α-pinene) were selected for verification and found to considerably increase the terpenoids production of A. camphorata and demonstrate the regulation of mRNA expression levels of nine key genes in the mevalonate pathway via RT-qPCR. This study is beneficial for elucidating the terpenoids synthesis mechanism in A. camphorata.

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Section: Discussionmentioning

confidence: 95%

Section: ■ Discussionmentioning

confidence: 99%

Characterization of Cinnamomum kanehirae Extract-Stimulated Triterpenoids Synthesis in Submerged Fermentation of Antrodia camphorata via Untargeted Metabolomics

Luo

Lü

et al. 2023

J. Agric. Food Chem.

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“…Although high BOD could be efficiently reduced by activated sludge or anaerobic digestion, from the point of view of source recycling, how to reuse thin stillage deserves study. Thus, many studies have been carried out to transform thin stillage to a value-added product such as biomass, polysaccharides, triterpenoids, and so on [20][21][22][23][24][25][26][27]. The objective of this study was to investigate the feasibility of reusing thin stillage for liquid cultures of A. cinnamomea.…”

Section: Introductionmentioning

confidence: 99%

Revised Manuscript Submitted to Asian Journal of Biotechnology and Bioresource Technology Enhancement of Triterpenoids formation of Antrodia cinnamomea in thin Stillage-submerged Culture by Shifting the Shaking Rate and Adding Soybean Oil

Chiu

Yang

2018

AJB2T

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Antrodia cinnamomea is an endemic medical mushroom in Taiwan. It is well known that the major effective components in this medical fungus are polysaccharides, triterpenoids, and steroids; it is found only in Taiwan. Its anti-tumour activity remains Current research interest is focused on its anti-tumour activity from secondary metabolites of triterpenoids. However, the main disadvantage of mycelia produced by liquid cultures is the quite small amount of triterpenoid. In this study, various cultivation strategies were tested to investigate their effects on the production of triterpenoids in thin stillage submerged cultures of A. cinnamomea. The results indicate that when thin stillage was reused in shaking flask culture at a constant speed, crude triterpenoids production reached the level of 43.04 mg/L at a low inoculum size of 1% at d-20, which was twice that of the control of high inoculum size of 10%. This study demonstrates that diminishing inoculum size has a

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Effect of Chinese Herb Extract on the Formation of Triterpenoids in Shake-Flask Cultures of Antrodia cinnamomea

Cited by 2 publications

References 27 publications

Characterization of Cinnamomum kanehirae Extract-Stimulated Triterpenoids Synthesis in Submerged Fermentation of Antrodia camphorata via Untargeted Metabolomics

Characterization of Cinnamomum kanehirae Extract-Stimulated Triterpenoids Synthesis in Submerged Fermentation of Antrodia camphorata via Untargeted Metabolomics

Revised Manuscript Submitted to Asian Journal of Biotechnology and Bioresource Technology Enhancement of Triterpenoids formation of Antrodia cinnamomea in thin Stillage-submerged Culture by Shifting the Shaking Rate and Adding Soybean Oil

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