Winter green manure potentially uptake large amount of phosphorus (P) and potassium (K) from the soil. However, the mechanism underlying nutrient uptake in green manure varieties remain elusive. This study illustrates the P and K uptake potential in different leguminous and non-leguminous species through integrated approaches. The designed studies evaluated the 10 green manure crops included five leguminous species (i.e smooth vetch hairy vetch, common vetch, lathyrus sativus, and milk vetch) and five non-leguminous species (i.e February orchid, chinese radish, forage radish, rapeseed and ryegrass) were planted. Experimental results indicated that the smooth vetch had highest P and K uptake capabilities among all leguminous species. While, rapeseed and chinese radish greatly P and K obtain among all non-leguminous treatments. Therefore various species also affected soil P and K availability and soil enzymes. Soil enzymatic activities including phosphatase (150.7%) and N-acetyl-glucosaminidase (95.4%) were elevated in lathryus sativus compared to the hairy vetch and common vetch respectively. The highest β-glucosidase (95.2%) and leucine-aminopeptidase (107.6%) activities accumulated in lathyrus sativus greater than the smooth vetch in all legumes species. In non-leguminous treatments phosphatase (500.5%), β-glucosidase (424.4%), and N-acetylglucosaminidase (256.3%) activities were improved in chinese radish as compared to the rapeseed. In constrast ryegrass was promoted greatest leucine-aminopeptidase activity by 182.7% higher than forage radish. However soil enzymes significanlty improve soil nutrient status. As a conclusion smooth vetch, rapeseed, and Chinese radish varieties have more P and K obtain capacity and forage radish could enhance soil enzyme activities. It is suggested these varieties might be a better choice in green manuring practice for improving nutrient management and soil nutrient quality in agro-ecosystems.