Effect of chloride and diamide on sheep kidney, lung and serum angiotensin converting enzyme

Abstract

The effect of chloride and diamide on angiotensin converting enzyme (ACE) activity from sheep kidney, lung and serum was investigated by using Hip-His-Leu as substrate. Optimum chloride concentrations were 400 -1000 mM for kidney, 700 mM for lung and 1000 mM for serum. Optimum chloride concentration increased ACE activity of serum and lung 1.70 folds and 2.73 folds respectively of the activity at physiological chloride concentrations, suggesting that the effect of salt on blood pressure may be due to the chloride sensitivity of ACE. The difference in effect of chloride on lung, kidney and serum ACE suggest that each tissue ACE has unique three dimentional structure. Increased pulmonary and serum ACE activity on pretreatment with diamide indicates that tissue oxidation may alter blood pressure.

An in Vitro Study of Cinnamomum Zeylanicum as Natural Inhibitor of Angiotensin-Converting Enzyme (Ace) on Sheep (Ovis Aries) Tissues

“…The powder of the plant part is extracted with methanol using a Soxhlet apparatus (60-80°C, 1:50 w/v) until the last extract is colorless. The combined extract is filtered and the filtrate is concentrated and evaporated on a boiling water bath until to afford the 50% extract [21]. Tissue ACE activity in the presence of inhibitor (C. zeylanicum [Bark]) was measured from Cushman and Cheung method [17], with 25 µl of methanolic exact of inhibitor.…”