Effect of cholesterol on the rigidity of saturated and unsaturated membranes: fluctuation and electrodeformation analysis of giant vesicles

Gracià, Rubèn Serral; Bezlyepkina, Natalya; Knorr, Roland L.; Lipowsky, Reinhard; Dimova, Rumiana

doi:10.1039/b920629a

Cited by 324 publications

(390 citation statements)

References 69 publications

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“…38, point to an increase in membrane stiffness, impairing the lipid zipper. Following a study on DOPC vesicles, cholesterol has no (significant) effect on bending rigidity (39). However, we found a significantly lower occurrence of the lipid zipper in cholesterol-free GUVs.…”

Section: Discussionmentioning

confidence: 71%

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A lipid zipper triggers bacterial invasion

Eierhoff

Bastian

Thuenauer

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

128

161

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Significance Entry of bacteria into host cells critically depends on their proper engulfment by the plasma membrane. So far, actin polymerization has been described as a major driving force in this process. However, our study reveals that the interaction of the bacterial surface lectin LecA with the host cell glycosphingolipid Gb3 is fully sufficient to promote engulfment of Pseudomonas aeruginosa , whereas actin polymerization is dispensable. Hence, the formation of a “lipid zipper” represents a previously unidentified mechanism of bacterial uptake and demonstrates that bacterial pathogens have evolved lipid-based invasion strategies that may function in addition to protein receptor-based ones. Furthermore, by identifying the LecA/Gb3 interaction as the major invasion-promoting factor, our study provides new targets for drugs that may prevent bacterial invasion and dissemination.

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Section: Discussionmentioning

confidence: 71%

“…However, we found a significantly lower occurrence of the lipid zipper in cholesterol-free GUVs. Interpreting our results in the context of the above-mentioned report (39), cholesterol might affect the lipid zipper on GUVs by mechanisms other than membrane stiffness.…”

Section: Discussionmentioning

confidence: 95%

A lipid zipper triggers bacterial invasion

Eierhoff

Bastian

Thuenauer

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

128

161

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“…Chol can increase rigidity and packing density of PC molecules, thus to decrease elasticity of vesicle bilayers. 23,24) In contrast, the incorporation of edge activator e.g., CPC which have a high radius of curvature can increase deformability of the vesicle bilayers. MEN can decrease the orderly lipid microstructure by insertion and thus increase the fluidity or elasticity of vesicle bilayer.…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Novel Ultradeformable Liposomes: Menthosomes, Transfersomes and Liposomes for Enhancing Skin Permeation of Meloxicam

Duangjit

Obata

Sano

et al. 2014

Biological & Pharmaceutical Bulletin

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In the present study, novel ultradeformable liposomes (menthosomes; MTS), deformable liposomes (transfersomes; TFS) and conventional liposomes (CLP) were compared in their potential for transdermal delivery of meloxicam (MX). MTS, TFS and CLP were investigated for size, size distribution, zeta potential, elasticity, entrapment efficiency and stability. In vitro skin permeation using hairless mice skin was evaluated. Vesicular morphology was observed under freeze-fractured transmission electron microscopy (FF-TEM). Intrinsic thermal properties were performed using differential scanning calorimetry (DSC) and X-ray diffraction. The skin permeation mechanism was characterized using confocal laser scanning microscopy (CLSM). The results indicated that the difference in physicochemical characteristics of MTS, TFS and CLP affected the skin permeability. MTS and TFS showed higher flux of MX than CLP. CLSM image showed deformable vesicles mechanism for delivery of MX across the hairless mice skin. Our study suggested that ultradeformable and deformable liposomes (MTS and TFS) had a potential to use as transdermal drug delivery carriers for MX.Key words menthosome; transfersome; liposome; skin permeation; meloxicam; menthol Meloxicam (MX), a cyclooxygenase-2 inhibitor nonsteroidal anti-inflammatory drug (NSAID), is used to treat rheumatoid arthritis, osteoarthritis and other joint diseases. The injectable and oral administrations of NSAID drugs are not appropriate for needle-phobia and peptic ulcer patients. Moreover, the major limitation of MX is its low aqueous solubility (0.012 mg/mL in water and 0.086×10 −2 mg/mL in 0.1 M HCl) 1) with log P 1.91 and 0.07 at pH 5.0 and 7.4, respectively. MX delays its absorption from gastrointestinal tract (GI) and its prolonged use is associated with the incidence of GI side effects (bellyache, indigestion, ulceration and bleeding).2) If MX could be delivered without incidence of these limitations, MX administration would become safer and more acceptable. MX is suitable for development as a transdermal delivery candidate.Transdermal drug delivery or skin delivery has become a global priority because the conventional drug administration is associated with numerous limitations. Liposomes are one of potential strategies that utilize for skin delivery of hydrophilic drugs, 3) lipophilic drugs, 4) protein 5) and macromolecule.6) Although last decades, some design of conventional liposomes are of little or no value as carriers for transdermal drug delivery, but rather remain confined to the upper layer of the stratum corneum. However, recent approaches in vesicular modulating drug delivery through skin have resulted in many designs of novel vesicular carriers e.g., deformable liposomes (transfersomes), 7) niosomes, 8) ethosomes, 9) invasomes, 10) flexosomes 11) and menthosomes. 12) Transfersomes are the first generation of elastic vesicles introduced by Cevc and Blune 7) and consist mainly of phospholipids and an edge activator or a single-chain surfactant which having a high radius of curv...

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“…In ternary mixtures of unsaturated and saturated lipids and cholesterol, formation of raft-like domains enriched in the two latter compounds is observed [37,38]. Cholesterol makes the spontaneous curvature of lipid monolayers more negative [39] and can modify the elastic properties of lipid bilayers [40,41]. The lipid composition of biological membranes varies considerably.…”

mentioning

confidence: 99%

Stalk structures in lipid bilayer fusion studied by x-ray diffraction

Aeffner¹

2012

Göttingen Series in X-Ray Physics

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Effect of cholesterol on the rigidity of saturated and unsaturated membranes: fluctuation and electrodeformation analysis of giant vesicles

Cited by 324 publications

References 69 publications

A lipid zipper triggers bacterial invasion

A lipid zipper triggers bacterial invasion

Comparative Study of Novel Ultradeformable Liposomes: Menthosomes, Transfersomes and Liposomes for Enhancing Skin Permeation of Meloxicam

Stalk structures in lipid bilayer fusion studied by x-ray diffraction

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