Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm

Amorim, E. A. M.; Graham, James K.; Spizziri, B.; Meyers, M.; Torres, Ciro Alexandre Alves

doi:10.1016/j.cryobiol.2008.12.007

Cited by 61 publications

(38 citation statements)

References 34 publications

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“…In this study, 7-DCLC increased percentages of progressive and total motility after thawing. Similar results were found in other cholesterol conjugates (2,23). Also some authors found improvement both in motility and sperm membrane integrity after incubating the semen with CLC prior the cryopreservation (3, 21, 34).…”

Section: Discussionsupporting

confidence: 73%

“…Sperm susceptibility to cryopreservation is explained by membrane phospholipid ratio and membrane cholesterol to phospholipid composition (15). Besides, changing lipid structure of sperm membrane affects sperm freezing (2). It means that sperms having low cholesterol: phospholipid rates from boars, stallions, rams and bulls are more sensitive to the cryo damage than sperms from * Bu çalışma "Akkaraman koçlarda spermanın kolesterol ve 7-dehidrokolesterol ile dondurulması ve değerlendirilmesi" adlı tezden üretilmiştir.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

̇İnanç¹,

Uysal²,

Ata³

2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The experiment was conducted to determine the in vitro results of 7-dehydrocholesterol loaded cyclodextrin compound (7-DCLC) addition to tris extenders for freezing ram semen. Three Akkaraman rams, local species in Turkey, were used. Semen samples were extended with tris containing 0 mg (control), 1.5 mg, 2.5 mg and 3.5 mg/120x10 6 7-DCLC and they were equilibrated to 4°C and frozen in mini straws then stored in liquid nitrogen. Straws were thawed at 37°C for 30 s in water bath for spermatological examination. Statistically significant differences were observed between 7-DCLC and control groups in terms of acrosome integrity, CASA motility, HOS test and viability after thawing (P <0.001). In all these parameters, control group had the lowest percentages. There was no statistically significant difference between groups in terms of DNA fragmentation, abnormal spermatozoa ratio, mitochondrial activation, lipid peroxidation and apoptosis level (P> 0.05). In conclusion, it can be said that adding 7-DCLC to Akkaraman ram semen improved cell membrane cryosurvival. Besides, 7-DCLC can be used in tris extender instead of cholesterol loaded cyclodextrin (CLC) compounds for sperm cryopreservation.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

̇İnanç¹,

Uysal²,

Ata³

2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Em contraste, no sêmen bovino os espermatozoides tratados ou não com CCC são equivalentes, tanto na sua capacidade de fertilização como no tempo necessário para capacitação (PURDY; GRAHAM, 2004a).Purdy e Graham (2004a) não verificaram diferenças in vitro nas taxas de clivagem e blastocisto, e também in vivo, com taxas de gestação de 59 e 50%, com e sem tratamento com CCC respectivamente.Isto sugere que o aumento do número de espermatozoides que sobrevivem após o congelamento (PURDY; GRAHAM, 2004b;GRAHAM, 2006;AMORIM et al,2009) pode melhorar as taxas de fertilidade ou permitir um número menor de espermatozoides utilizados por dose inseminante, sem reduzir a fertilidade, sendo necessário maiores ensaios para confirmação desta hipótese (PURDY; GRAHAM, 2004a).…”

Section: Fertilidade Do Sêmen Tratado Com CCCunclassified

Uso De Colesterol Na Criopreservação Espermática E Fertilidade: Uma Revisão

Boscarato

Martins

Pinho

et al. 2015

ArqVet

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MARTINS, L. F.; PINHO, R. O.; MACEDO, G. G.; ORLANDINI, C. F. Uso de colesterol na criopreservação espermática e fertilidade: uma revisão. Arq. Ciênc. Vet. Zool. UNIPAR, Umuarama, v. 17, n. 2, p. 145-150, abr./ jun. 2014. RESUMO:A criopreservação é um método amplamente utilizado para o armazenamento de células espermáticas em longo prazo. Essa técnica facilita a difusão e a disponibilidade de material genético a ser utilizado tanto na produção in vitro (PIV) como na inseminação artificial, biotecnologia aplicada na maioria das espécies domésticas. Pesquisas têm desenvolvido estratégias para otimizar a utilização do sêmen por meio da redução das injúrias ocorridas durante o processo de criopreservação, e vem conseguindo melhorar os parâmetros espermáticos pós-descongelação com a adição de ciclodextrina carregada com colesterol (CCC) ao sêmen puro ou previamente diluído das diferentes espécies estudadas. Apesar dos resultados promissores, melhor fertilidade do sêmen tratado com CCC tem sido demonstrada somente in vitro, enquanto que in vivo os resultados ainda não são compensatórios, necessitando maiores investigações para tornar viável a aplicação comercial dessa técnica. O objetivo dessa revisão foi compilar alguns resultados dos principais pesquisadores na área, expondo o estado atual e as perspectivas para melhorias da fertilidade do sêmen tratado com CCC. PALAVRAS-CHAVE: Ciclodextrina. Inseminação artificial. Membrana plasmática. Semen. USE OF CHOLESTEROL IN SPERM CRYOPRESERVATION AND FERTILITY: A REVIEWABSTRACT: Cryopreservation is a method widely used for the long-term storage of sperm cells. This technique facilitates the dissemination and availability of genetic material to be used in both in vitro production (IVP) and in artificial insemination, the biotechnology applied to most domestic species. Research has developed strategies to optimize the use of semen through the reduction of injuries occurred during the cryopreservation process, having succeeded in improving post-thaw sperm parameters with the addition of cholesterol-loaded cyclodextrin (CLC) to pure or previously diluted semen samples from the different species studied. Despite the promising results, better fertility of semen treated with CLC has only been demonstrated in vitro, while in vivo results are not yet feasible, requiring further investigation to result in viable commercial application of such technique. The objective of this review is to compile a few results from the leading researchers in the area, exhibiting the current state and prospects for improving the fertility of semen treated with CLC. KEYWORDS: Cyclodextrin. Artificial insemination. Plasma membrane. Semen. USO DE COLESTEROL EN LA CRIOPRESERVACIÓN ESPERMÁTICA Y FERTILIDAD: UNA REVISIÓNRESUMEN: La criopreservación es un método ampliamente utilizado para el almacenamiento de células espermáticas a largo plazo. Esa técnica facilita la difusión y la disponibilidad de material genético a ser utilizado tanto en la producción in vitro (PIV) como en la inseminación artificial, bio...

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“…Inducing cholesterol in sperm plasma membrane with cyclodextrin molecules (CLCs) (44) enhanced the cryotolerance in rams (45), bulls (46)(47)(48), bucks (49), elephants (50), and stallions (51). This might be due to the fact that increased cholesterol in the sperm cell membrane enhanced the osmotic tolerance and inhibited the acrosome reaction (52).…”

Section: β-Cyclodextrinmentioning

confidence: 99%

Cholesterol efflux from sperm: approaches and applications

Naseer¹,

Ahmad²,

Aksoy³

2014

Turk J Vet Anim Sci

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Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides insight to researchers designing new procedures for sperm preparation to be used in assisted reproductive technology.

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Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm

Cited by 61 publications

References 34 publications

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

Uso De Colesterol Na Criopreservação Espermática E Fertilidade: Uma Revisão

Cholesterol efflux from sperm: approaches and applications

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