Effect of Chondroitin Sulfate on Blood Serum Cytokine Profile during Carrageenan-induced Edema and Monoiodoacetate-induced Osteoarthritis in Rats

Korotkyi, Oleksandr; Vovk, A.; Blokhina, O.; Dvorshchenko, K.; Falalyeyeva, Tetyana; Abenavoli, Ludovico; Li, Ostapchenko

doi:10.2174/1574887113666181102111247

Cited by 16 publications

(8 citation statements)

References 24 publications

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“…Despite an increase in the level of TGFbeta in MIA-OA that correlates with an increased expression of the TGF-β gene in cartilage [57], our other study showed negative histological changes [19]. This may indicate insufficient compensatory effort against a significant increase of pro-inflammatory cytokines in the cartilage and blood [58], and may also be associated with fibrosis and cartilage degeneration [59].…”

Section: Resultsmentioning

confidence: 78%

Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic

et al. 2020

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To identify the effect of probiotic (PB) on cytokines profile in knee cartilage of rats with experimental osteoarthritis (OA). Methods. The animals of 1 st (Сontrol) and 3 rd (PB) groups got 50 µL of saline to both hind knees. The animals of 2 nd (MIA-OA) and 4 th groups (MIA-OA-PB) got single injection of 1 mg monoiodoacetate (MIA) dissolved in 50 µL of saline to knee, after which MIA-OA developed for 28 days. PB feeding [was] provided daily for 14 days during the MIA-OA progress. The levels of cytokines (IL-1β, TNF-α, IL-6, IL-8, IFN-γ, IL-4, IL-10, TGF-β, IGF-1) were measured in cartilage homogenates by [the] enzyme-linked immunosorbent assay (Biotrak ELISA System, GE Healthcare, USA). Results. MIA-OA caused [an] increase in the levels of IL-1β, TNF-α, IL-6, IL-8, IFN-γ, IL-10, TGF-β, decreased the level of IGF-1 and did not change the level of IL-4, compared to Control. PB during MIA-OA increased the level of IGF-1, decreased the levels of IL-1β, TNF-α, IL-6, IL-8, IFN-γ, TGF-β, compared to MIA-OA, but did not reach the Control values (unlike IL-4 and IL-10 that equaled to Control). Conclusions. MIA-OA caused significant changes in the levels of studied cytokines in knee cartilage. An application of PB has positive local anti-inflammatory effect in cartilage tissue of rats with MIA-OA.

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Section: Resultsmentioning

confidence: 78%

Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic

et al. 2020

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“…GT activity decreased in 1.6 times in blood serum of rats with MIA-OA, compared to control group (Table 3). Decreasing of GT activity may be a response to an excess of toxic oxygen metabolites, due to the development of the inflammatory process [26,49]. GT is highly specific to reduced glutathione, and a decrease in GSH pool can cause a decrease in enzyme activity [50].…”

Section: Resultsmentioning

confidence: 99%

“…Induction of monoiodoacetate (MIA) into the patellofemoral joint of an experimental rat model induces histopathological changes in the knee joint that similar to those during tibiofemoral OA [23]. MIA-induced model of OA (MIA-OA) in rats is widely used to describe morphology, loss of mobility and biochemistry of the disease [24,25], significant changes of cytokine profile [26].…”

mentioning

confidence: 99%

Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Korotkyi¹,

Dvorshchenko²,

Vovk³

et al. 2019

Ukr.Biochem.J

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Osteoarthritis (OA) is a widespread pathology of the musculoskeletal system. OA may associate with a wide range of disorders, that lead to the development of various strategies on how to prevent and treat the disease. recent studies discussed interactions between the microbiome and a wide range of pathologies, including OA. In this study, we investigated the effect of probiotic cultures on oxidative/antioxidant balance in blood of rats during OA. Experimental OA was induced by a single injection of sodium monoiodoacetate into the knee joint. A probiotic composition (Multiprobiotic Simbiter®) was administered by peroral catheter once per day for 14 days. We investigated the next parameters: expression of Nos2 gene in the blood, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione transferase activity, glutathione reductase activity, contents of superoxide, hydrogen peroxide, TBA-reactive compounds, oxidized and reduced glutathione in the serum of the blood. Monoiodoacetate-induced OA caused significant changes on oxidative/antioxidant balance in the blood of rats: increasing of the contents of superoxide anion radical, hydrogen peroxide, thiobarbituric acid-reactive compounds, oxidized glutathione, upregulating of Nos2 expression, increasing of catalase activity; conversely, superoxide dismutase activity, glutathione peroxidize activity, glutathione transferase activity, glutathione reductase activity, the content of reduced glutathione were significantly decreased, compared to control group. Administration of probiotics to animals with OA led to positive changes in the studied parameters approaching the values of control group (some of them were statistically significant). K e y w o r d s: experimental osteoarthritis, microbiome, free radical processes, glutathione.

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damage to the liver and the intestine can result from the side effects of drugs such as: nonsteroidal anti-inflammatory glucocorticoids, salazopyrazins, statins, antihypertensives, proton pump inhibitors etc. [13][14][15][16][17].The practical importance of this paper consists in clarifying the mechanisms of progression of steatohepatitis and chronic colitis, improving the efficacy of this pathology treatment with the use of a modern nano-hepatoprotector. While there are data on treatment of hepatic and/or intestinal diseases [6], this paper emphasizes treatment of patients with steatohepatitis and concomitant chronic colitis.The study objective is evaluation of the treatment efficacy of steatohepatitis with concomitant chronic colitis using phosphatidylcholine and glycyrrhizic acid.

MATERIALS AND METHODSEighty-seven patients with steatohepatitis in combination with chronic colitis were examined in the gastroenterology day hospital of the advisory diagnostic center in Podolsk district of the city of Kiev, including those with non-alcoholic steatohepatitis (NASH) -38 (44%), alcoholic steatohepatitis (ASH) -28 (32%), drug-induced liver injury (DILI) -21 (24%).

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mentioning

confidence: 99%

“…damage to the liver and the intestine can result from the side effects of drugs such as: nonsteroidal anti-inflammatory glucocorticoids, salazopyrazins, statins, antihypertensives, proton pump inhibitors etc. [13][14][15][16][17].…”

mentioning

confidence: 99%

Increasing efficacy of treatment in patients with steatohepatitis and concomitant chronic colitis

Manzhalii

Moyseyenko

Falalyeyeva

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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This paper presents the results of a study of patients with nonalcoholic steatohepatitis, alcoholic steatohepatitis, and drug-induced liver injury with concomitant colitis and the development of biliary insufficiency that manifests as a significant decrease in the volume of bile acids and bile. In patients with liver injury and concomitant colitis, changes in intestinal microflora were marked. After a combination therapy of this pathology with phosphatidylcholine and glycyrrhizic acid, an improvement of biochemical, clinical indicators was observed, as well as a decrease in fatty degeneration and reduction of elastographic indicators of liver fibrosis. The introduction of the drug formulations phosphatidylcholine and glycyrrhizic acid in the complex of conservative treatment of liver injury with concomitant colitis helps to prevent hepatic and intestinal complications and to increase the treatment efficacy of these diseases.

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Effect of Chondroitin Sulfate on Blood Serum Cytokine Profile during Carrageenan-induced Edema and Monoiodoacetate-induced Osteoarthritis in Rats

Cited by 16 publications

References 24 publications

Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic

Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic

Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Increasing efficacy of treatment in patients with steatohepatitis and concomitant chronic colitis

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