Effect of Chronic Toxicity Studies of Sappan Wood Extract on The Kupffer Cells Number in Rats (Rattus novergicus)

Abstract

Sappan wood (Caesalpinia sappan L.) is a plant used by the community to mix drinking. The antioxidant index of Sappan wood extract has a higher value than commercial antioxidants to counteract oxidative free radicals and improve the body's defense system. The use of Sappan wood drinking in society is often used in daily so it is necessary to do a chronic toxicity test to observe the response to prolonged use of the system in the body. Kupffer cells are an important part of the innate immune system, acting as “scavengers” and phagocytes. A study that aims to see the chronic toxicity studies of the ethanolic extract of Sappan wood on the number of Kuffer cells in the liver has been carried out on 60 Wistar rats divided into 12 groups. The group was the negative control group, doses 100 mg/kgBW, 200 mg/kgBW, 300 mg/kgBW, 400 mg/kgBW and 500 mg/kgBW of extract Sappan wood which were divided into male and female groups. Each group was given ethanol extract of Sappan wood for one year orally. The results showed a significant increase in Kupffer cells in the female group at a dose of 100 mg/kgBW and the male group at 200 mg/kgBW with significant values, respectively, p<0.001 p = 0.004.

Effects of Dietary Supplementation with Caesalpinia sappan Linn. Extract for Promoting Flock Health and Performance in Late-Phase Laying Hens

“…Additionally, no mortality or signs of intoxication were observed following exposure to various dietary regimens. Furthermore, no chronic, subacute, or acute toxicity of C. sappan have been extensively reported [54][55][56].…”

Kajian Aktivitas Antioksidan Dari Kayu Secang (Caesalpinia Sappan L.)

“…Meningkatnya jumlah sel Kupffer menandakan bahwa penggunaan ekstrak secang dapat meningkatkan sistem imun tubuh. Meningkatnya jumlah sel Kupffer disebabkan oleh senyawa polifenol yang terdapat pada ekstrak secang (Khristian et al, 2022).…”

“…Metode Hasil Pengujian Khristian et al, (2022) ANOVA dan uji post hoc Duncan menggunakan software SPSS 26 Terjadi peningkatan sel Kupffer yang signifikan pada kelompok Tikus betina (Khristian et al, 2022). Harijono et al, (2021) TED Pemberian wedang uwuh pada tikus memiliki efek yang signifikan dalam menurunkan kadar glukosa dan juga efektif dalam mengontrol kenaikan berat badan pada tikus (Harijono et al, 2021).…”