Effect of CMC Concentration on Cell Growth Behavior of PVA/CMC Hydrogel

Effect of polyethylene glycol molecular weight on cell growth behavior of polyvinyl alcohol/carboxymethyl cellulose/polyethylene glycol hydrogel

“…The pore size was then determined by using the optical microscope equipped with the iSolution Lite image software. [13][14][15][16][17][18][19][20][21] Compressive strength of the irradiated PVA/ CMC hydrogels was examined by using an Instron 5564 with a crosshead speed of 10 mm/min. The specimen with a diameter of 15 mm and a thickness of 5 mm was fabricated and the strength was determined at a point where the specimen thickness decreased by 50%.…”

“…The extract test method was conducted on the irradiated PVA/CMC/PEG hydrogels to evaluate the potential of cytotoxicity according to the International Organization for Standardization (ISO 10993-5). [13][14][15][16][17][18][19][20][21] The hydrogels were extracted aseptically in single strength minimum essential medium (1× MEM, Dulbecco's modified Eagle's medium [Gibco, Paisley, United Kingdom] with 10% fetal bovine serum [Gibco, Paisley, United Kingdom] and 1% penicillin-streptomycin) with serum. The ratio of the hydrogels to the extraction vehicle was 0.1 g/ml (ISO 10993-12).…”

“…Detailed experimental procedures are described elsewhere. [4,5,[13][14][15][16][17][18][19][20][21] Cell counting kit-8 (CCK-8, Dojindo Molecular Technologies, Inc., Rockville, MD, USA) is used for the assay of cell proliferation. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation.…”

“…CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation. [13][14][15][16][17][18][19][20][21] Watersoluble tetrazolium salts were reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.…”

“…Scratch assay is a simple method to study the coordinated movement of a cell population. [14,[22][23][24][25][26][27][28] Fibroblast cell (L-929, ATCC) was used to observe the cell migration by artificially forming a gap between cells. A scratch (cell-free area) was created on the surface of L-929 cells by using a 200 μl tip and a sterilized ruler.…”