Effect of co-administration of<i>Hypoxis hemerocallidea</i>extract and antiretroviral therapy (HAART) on the histomorphology and seminal parameters in Sprague Dawley rats

Jegede, Ayoola Isaac; Offor, Ugochukwu; Onanuga, Ismail Olasile; Naidu, Edwin Coleridge Stephen; Azu, Onyemaechi Okpara

doi:10.1111/and.12640

Cited by 14 publications

(22 citation statements)

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“…Sperm morphology was accessed by staining dry smeared diluted epididymal fluid on a glass slide with eosin‐nigrosine staining and observed under a light microscope (Leica DM 500) at 400× magnification. The number of normal spermatozoa, spermatozoa with abnormal heads, spermatozoa with abnormal tail, and spermatozoa with abnormal midpiece was recorded in percentage (Jegede et al ., ).…”

Section: Methodsmentioning

confidence: 97%

“…Hormonal perturbation and androgen deficiencies have also been associated with HAART (Bhangoo & Desai, ; Rama et al ., ; Zavattaro et al ., ), resulting in male gonadal dysfunction (Lachâtre et al ., ). This is mediated by a dysregulation of the hypothalamo‐pituitary‐testicular axis (Collazos et al ., ; Jegede et al ., ). In a cohort study of male homosexual, bisexual, and HIV + men, the use of HAART was associated with raised serum estradiol levels (Lamba et al ., ; Scanavino, ).…”

Section: Introductionmentioning

confidence: 97%

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Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

et al. 2017

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SUMMARYHighly active antiretroviral therapy has evolved over the years, leading to a boost in the quality of life in people living with HIV and AIDS. However, growing evidence has shown that highly active antiretroviral therapy has deleterious effects on the testes and the overall reproductive capacity. Therefore, this study is to determine the adjuvant potential of Naringenin on highly active antiretroviral therapy-induced perturbations in fertility of male Sprague-Dawley rats. Thirty adult male Sprague-Dawley rats were divided into six groups viz -Control; H: 30 mg/kg of highly active antiretroviral therapy (EFV, 600 mg + FTC, 200 mg + TDF, 300 mg); N40: Naringenin, 40 mg/kg; N80: Naringenin, 80 mg/kg; HN40: highly active antiretroviral therapy + Naringenin, 40 mg/kg; HN80: highly active antiretroviral therapy + Naringenin, 80 mg/kg. The rats were euthanized after 4 weeks. Results showed that there was a significant decrease in sperm count (p < 0.001), spermatozoa with normal morphology (p < 0.001) and progressive sperm motility (p < 0.05) of H compared to the control and the HN groups. Likewise, fragmentations increased (p < 0.05) in tail lengths of sperm DNA in H compared to control. HN40 and HN80 decreased tail lengths compared to H (p < 0.001). There was also a decrease in %tail DNA and tail moment in HN40 (p < 0.001) compared to H. Luteinizing hormone significantly increased (p < 0.05) in HN40, HN80, and N40 (p < 0.001) but decreased in H (p < 0.05) compared to control. The diameter of the seminiferous tubules also decreased (p < 0.05) in H compared to control, N80, and HN40. Likewise, the area of the seminiferous tubules in group H decreased (p < 0.05) compared to N80 and HN80. The seminiferous tubules epithelium increased (p < 0.05) in N40 and HN40 compared to H. This study establishes that highly active antiretroviral therapy has deleterious effects on the testicular microanatomy, sperm parameters, and sperm DNA of Sprague-Dawley rats, which may impair fertility but Naringenin is a potential complimentary adjuvant.

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Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

et al. 2017

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“…Thirty (30) adult male and 18 female Sprague Dawley (Aitken & Roman, ) rats, weighing 200–220 g (Jegede, Offor, Onanuga, Naidu, & Azu, ), were obtained from the animal house of the Biomedical Resource Unit, University of KwaZulu—Natal, South Africa. They were housed in standard cages under controlled environmental conditions (25°C and a 12‐hr light/dark cycle).…”

Section: Methodsmentioning

confidence: 99%

Testicular microanatomical and hormonal alterations following use of antiretroviral therapy inSprague Dawleyrats: Role of Naringenin

et al. 2018

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Human immunodeficiency virus-infected man may require assisted reproductive technology not just for safer conception but also due to subfertility. The study investigated the effect of antiretroviral drugs on the fertility potentials of males and the possible protective role of Naringenin, using Sprague Dawley rats. Thirty adult male Sprague Dawley rats were grouped into-A: Distilled water; B: Highly Active Antiretroviral Therapy (HAART); C: Naringenin 40 mg/kg; D: Naringenin 80 mg/kg, E: HAART + Naringenin 40 mg/kg; F: HAART + Naringenin 80 mg/kg. The rats were euthanised after 10 weeks. Results showed a significant decrease in sperm count in group B when compared to the control and other groups. Spermatozoa with normal morphology also reduced significantly in the B group and progressive sperm motility reduced when compared to the control, D and the F group. The serum testosterone was not significantly different between groups A and B, however the groups C and D displayed significant increase when compared to groups A and B. The serum luteinising hormone was significantly higher in group B when compared to groups A, E and F. Our data suggest that Naringenin improves the male reproductive anatomy and function, therefore, it promises to be a beneficial adjuvant for mitigating HAART testicular and reproductive perturbations.

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“…Due to the high prevalence of AIDS and drug‐resistant strains, the demand for new antiviral drugs against AIDS is rising. Various medicinal plants are used in the treatment of AIDS such as Withania somnifera (Williams‐Orlando, ), Tinospora cordifolia (Husain et al, ), Moringa oleifera (Monera‐Penduka et al, ), Hypericum perforatum (Béjaoui et al, ), Silybum marianum (Lovelace et al, ), Allium sativum (Maroyi, ), Panax ginseng (Cho & Kim, ), Lobostemon trigonus (Koffuor et al, ), Emblica officinalis (Bhagat, ), Curcuma longa (Kim et al, ), Hypoxis hemerocallidea (Jegede, Offor, Onanuga, Naidu, & Azu, ), and Sutherlandia frutescens (Wilson et al, ). Herbal medicines are used for the treatment of viral infections since ancient times.…”

Section: Hiv/aids and Medicinal Plantsmentioning

confidence: 99%

“…Its pharmacological activity is adaptogenic (Cicero et al, 2004). Glatthaar, Sacher, and Esperester (2001) Silybum marianum (Lovelace et al, 2017), Allium sativum (Maroyi, 2014), Panax ginseng (Cho & Kim, 2017), Lobostemon trigonus (Koffuor et al, 2014), Emblica officinalis (Bhagat, 2014), Curcuma longa (Kim et al, 2014), Hypoxis hemerocallidea (Jegede, Offor, Onanuga, Naidu, & Azu, 2017), and Sutherlandia frutescens (Wilson et al, 2015). Herbal medicines are used for the treatment of viral infections since ancient times.…”

Section: Medicinal Plants Used In Influenzamentioning

confidence: 99%

Antiviral potential of medicinal plants against HIV, HSV, influenza, hepatitis, and coxsackievirus: A systematic review

Akram

Tahir

Shah

et al. 2018

Phytotherapy Research

222

170

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Viral infections are being managed therapeutically through available antiviral regimens with unsatisfactory clinical outcomes. The refractory viral infections resistant to available antiviral drugs are alarming threats and a serious health concern. For viral hepatitis, the interferon and vaccine therapies solely are not ultimate solutions due to recurrence of hepatitis C virus. Owing to the growing incidences of viral infections and especially of resistant viral strains, the available therapeutic modalities need to be improved, complemented with the discovery of novel antiviral agents to combat refractory viral infections. It is widely accepted that medicinal plant heritage is nature gifted, precious, and fueled with the valuable resources for treatment of metabolic and infectious disorders. The aims of this review are to assemble the facts and to conclude the therapeutic potential of medicinal plants in the eradication and management of various viral diseases such as influenza, human immunodeficiency virus (HIV), herpes simplex virus (HSV), hepatitis, and coxsackievirus infections, which have been proven in diverse clinical studies. The articles, published in the English language since 1982 to 2017, were included from Web of Science, Cochrane Library, AMED, CISCOM, EMBASE, MEDLINE, Scopus, and PubMed by using relevant keywords including plants possessing antiviral activity, the antiviral effects of plants, and plants used in viral disorders. The scientific literature mainly focusing on plant extracts and herbal products with therapeutic efficacies against experimental models of influenza, HIV, HSV, hepatitis, and coxsackievirus were included in the study. Pure compounds possessing antiviral activity were excluded, and plants possessing activity against viruses other than viruses in inclusion criteria were excluded. Hundreds of plant extracts with antiviral effect were recognized. However, the data from only 36 families investigated through in vitro and in vivo studies met the inclusion criteria of this review. The inferences from scientific literature review, focusing on potential therapeutic consequences of medicinal plants on experimental models of HIV, HSV, influenza, hepatitis, and coxsackievirus have ascertained the curative antiviral potential of plants. Fifty-four medicinal plants belonging to 36 different families having antiviral potential were documented. Out of 54 plants, 27 individually belong to particular plant families. On the basis of the work of several independent research groups, the therapeutic potential of medicinal plants against listed common viral diseases in the region has been proclaimed. In this context, the herbal formulations as alternative medicine may contribute to the eradication of complicated viral infection significantly. The current review consolidates the data of the various medicinal plants, those are Sambucus nigra, Caesalpinia pulcherrima, and Hypericum connatum, holding promising specific antiviral activities scientifically proven through studies on experimental animal mo...

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Effect of co-administration ofHypoxis hemerocallideaextract and antiretroviral therapy (HAART) on the histomorphology and seminal parameters in Sprague Dawley rats

Cited by 14 publications

References 43 publications

Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

Testicular microanatomical and hormonal alterations following use of antiretroviral therapy inSprague Dawleyrats: Role of Naringenin

Antiviral potential of medicinal plants against HIV, HSV, influenza, hepatitis, and coxsackievirus: A systematic review

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