Effect of Coenzyme Q0 on biofilm formation and attachment-invasion efficiency of Listeria monocytogenes

Fan, Qiuxia; Zhang, Yanyan; Yang, Hua; Wu, Qian; Shi, Chao; Zhang, Chunyu; Xia, Xiaodong; Wang, Xin

doi:10.1016/j.foodcont.2018.02.047

Cited by 37 publications

(21 citation statements)

References 34 publications

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“…In both cases, the decreases followed a concentration-dependent trend. Fan et al (2018) also reported that coenzyme Q0 inhibited the attachment and invasion of L. monocytogenes CMCC 54004, while Xu et al (2015) found that the tannin-rich fraction from pomegranate rind homogenate remarkably reduced the ability of L. monocytogenes to adhere to and invade Caco-2 cells in a dose-dependent manner. Upadhyay et al (2012) showed that trans-cinnamaldehyde, carvacrol, and thymol down-regulated the transcription of genes encoding Listeria adhesion ( lmo1634, lmo1666 , and lmo1847 ) and invasion ( iap and lmo1076 ) proteins, resulting in decreased host-cell adherence and invasion.…”

Section: Discussionmentioning

confidence: 99%

“…To confirm this, a greater number of L. monocytogenes strains from various clinical and environmental sources should be tested. Fan et al (2018) reported that the MICs of coenzyme Q0 against L. monocytogenes strains ranged from 25—50 μg/mL, while the MICs of thymol and lactobionic acid against L. monocytogenes were 0.25 and 10 mg/mL, respectively (Chen and Zhong, 2017). Using the broth microdilution method, the MIC of epigallocatechin-gallate against L. monocytogenes was determined to be 200 μg/mL (Du et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The effect of TQ on the ability of L. monocytogenes to adhere to and invade host cells was investigated as previously described (Moroni et al, 2006). Human colon adenocarcinoma cell line Caco-2 was cultured as previously reported (Fan et al, 2018). For the assays, trypsin-treated Caco-2 cells were seeded into a 24-well tissue culture plate (10 5 cells per well) and incubated for 18 h under 5% CO 2 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

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Inhibitory Effect of Thymoquinone on Listeria monocytogenes ATCC 19115 Biofilm Formation and Virulence Attributes Critical for Human Infection

Miao

Liu

Zheng

et al. 2019

Front. Cell. Infect. Microbiol.

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This study aimed to determine the antimicrobial activity of thymoquinone (TQ) against Listeria monocytogenes , and to examine its inhibitory effects on biofilm formation, motility, hemolysin production, and attachment-invasion of host cells. The minimum inhibitory concentrations (MICs) of TQ against eight different L. monocytogenes strains ranged from 6.25—12.50 μg/mL. Crystal violet staining showed that TQ clearly reduced biofilm biomass at sub-MICs in a dose-dependent manner. Scanning electron microscopy suggested that TQ inhibited biofilm formation on glass slides and induced an apparent collapse of biofilm architecture. At sub-MICs, TQ effectively inhibited the motility of L. monocytogenes ATCC 19115, and significantly impacted adhesion to and invasion of human colon adenocarcinoma cells as well as the secretion of listeriolysin O. Supporting these findings, real-time quantitative polymerase chain reaction analysis revealed that TQ down-regulated the transcription of genes associated with motility, biofilm formation, hemolysin secretion, and attachment-invasion in host cells. Overall, these findings confirm that TQ has the potential to be used to combat L. monocytogenes infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Inhibitory Effect of Thymoquinone on Listeria monocytogenes ATCC 19115 Biofilm Formation and Virulence Attributes Critical for Human Infection

Miao

Liu

Zheng

et al. 2019

Front. Cell. Infect. Microbiol.

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“…1), is a key constituent part of coenzyme Q 10 . Coenzyme Q 0 has been reported possess antineoplastic, antiinflammatory and antimicrobial activities [5].…”

Section: Original Research Articlementioning

confidence: 99%

Single-step Syznthesis of Coenzyme Q0

Yan

Qiu

Zhang

et al. 2019

AJACR

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“…Coenzyme Q 0 (CoQ 0 ; 2,3-dimethoxy-5-methyl-1,4benzoquinone; C 9 H 10 O 4 ) is a benzoquinone compound extracted from the fungus Antrodia camphorata [28]. It has demonstrated anti-tumor, anti-in ammatory, and anti-angiogenic properties [29], as well as antibacterial activity against Staphylococcus aureus and Listeria monocytogenes [30,31]. In addition, CoQ 0 successfully inhibited bio lm formation by Salmonella enterica serovar Typhimurium [32].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

Guan¹,

Shi²,

Tong³

et al. 2020

Preprint

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Objectives：Here, we investigated the inhibitory effects of coenzyme Q0 (CoQ0) on biofilm formation and the expression of virulence genes by Cronobacter sakazakii. Results：We found that the minimum inhibitory concentration of CoQ0 against C. sakazakii strains ATCC29544 and ATCC29004 was 100 μg/mL, while growth curve assays showed that sub-inhibitory concentrations (SICs) of CoQ0 for both strains were 6.4, 3.2, 1.6 and 0.8 μg/mL. Assays exploring the inhibition of specific biofilm formation showed that SICs of CoQ0 inhibited biofilm formation by C. sakazakii in a dose-dependent manner, which was confirmed by scanning electron microscopy and confocal laser scanning microscopy analyses. CoQ0 inhibited the swimming and swarming motility of C. sakazakii and reduced its ability to adhere to and invade HT-29 cells. In addition, CoQ0 impeded the ability of C. sakazakii to survive and replicate within RAW 264.7 cells. Finally, real time polymerase chain reaction analysis confirmed that nine C. sakazakii genes associated with biofilm formation and virulence were down-regulated in response to CoQ0 treatment. Conclusion：Overall, our findings suggest that CoQ0 is a promising antibiofilm agent and provide new insights for the prevention and control of infections caused by C. sakazakii.

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Effect of Coenzyme Q0 on biofilm formation and attachment-invasion efficiency of Listeria monocytogenes

Cited by 37 publications

References 34 publications

Inhibitory Effect of Thymoquinone on Listeria monocytogenes ATCC 19115 Biofilm Formation and Virulence Attributes Critical for Human Infection

Inhibitory Effect of Thymoquinone on Listeria monocytogenes ATCC 19115 Biofilm Formation and Virulence Attributes Critical for Human Infection

Single-step Syznthesis of Coenzyme Q0

Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

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