Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata

Abstract

-The present study was performed to investigate the effects of the colchicine concentrations on chromosome doubling for producing of tetraploid plants of Codonopsis lanceolata, and its effect on plant morphology. A total of 180 individuals germinated from 16 treatment groups, were exposed to various concentrations (0.05-1.0% w/v) of colchicine for different soaking duration (3-24 hour). The highest numbers of tetraploid plants (3) were observed from the lowest concentration of colchicine (0.05%), and one (1) tetraploid plant was obtained from the 0.5% concentration group with a 6 hour treatment. However, no tetraploid individual was observed in any other treatment groups. The plant height of the diploid (18.1 ㎝) was slightly shorter than that of the tetraploid (13.4 ㎝). The fresh weight of the main root in the diploid (0.5 g) was four-fold higher than the tetraploid (2.2 g). The colchicine-treated plant regeneration rate in C. lanceolata was decreased when the plants were subjected to high concentration of colchicine. In particular, the highest number of tetraploid plants (5 and 3) was obtained from the lower concentration (0.05% and 0.1%) of colchicine for 6-hour treatment, which were a higher rate (29.4% and 30%) of regenerated tetraploid plants than other regenerated plants. As in the seed treatment result, the plant height of the diploid was significantly higher (10.4 ㎝) than tetraploid. The higher morphological changes were observed comparatively from tetraploid plants than the diploid.

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

“…The tetraploids resulted from colchicine treatment on Platanus acerifolia plants also have thicker and wider leaves [ 17 ]. In general, the tetraploid plants show an increase in organs and stomata size, trichome density, contents of secondary metabolites, and biomass properties [ 16 , 27 , 10 ].…”

Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

“…Ade and Rai (2010) reported that colchicine has the ability to induce polyploidy and can be used to convert sterile hybrids into fertile. The colchicine mutagen has been shown to induce polyploidy in various plants, including Scutellaria baicalensis (Gao et al 2002), Panax ginseng (Kim et al 2004), Artemisia annua (De Jesus, 2003;Banyai et al 2010;Rahman et al 2010) 2017), Codonopsis lanceolata (Kwon et al 2016), Glycyrrhiza glabra var. glandulifera (Bernard et al 2012), and Artemisia cina (Herawati et al 2015).…”

Seed Germination and Seedling Growth of Dendrocalumus brandisii in vitro, and the Inhibitory Mechanism of Colchicine

“…Survival rates of seedlings were lower than their germination rates in the media containing high colchicine content, indicating that high content of colchicine caused the seedling death. Kwon et al ( 2016 ) reported that a high concentration of colchicine significantly decreased seed germination of Codonopsis lanceolata . Another previous study also demonstrated that colchicine in high concentration had a negative effect on the germination of seeds, and survival plants recovered in the medical plant Salvia hains (Grouh et al, 2011 ).…”

“…Colchicine is an alkaloid contained in the seed or bulb of Colchicum autumnale belonging to the family of Liliaceae (Kwon et al, 2016 ). Colchicine is used in breeding and biological research to induce polyploidy and in tubulin-binding assays as a positive control (Trease and Evans, 1983 ).…”

“…It interferes with chromosome movement to poles and microtubules formation in the middle stage of cell division and induces polyploidization (Hadlaczky et al, 1983 ). The effect of colchicine for in vitro chromosome doubling is related to its concentration, method, and duration of treatment (Dhooghe et al, 2011 ; Aina et al, 2012 ; Moghbel et al, 2015 ; Kwon et al, 2016 ).…”